Cloned (Comment) | Organism |
---|---|
expression of genes NEQ205 and NEQ261 in Escherichia coli strain BL21(DE3), phylogenetic analysis | Nanoarchaeum equitans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Nanoarchaeum equitans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nanoarchaeum equitans | the enzyme is required for tRNA maturation removing an intervening sequence element from the tRNA precursor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nanoarchaeum equitans | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | substrate recognition mechanism | Nanoarchaeum equitans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is required for tRNA maturation removing an intervening sequence element from the tRNA precursor | Nanoarchaeum equitans | ? | - |
? | |
additional information | the enzyme cleaves noncanonical bulge-helix-bulge motifs of joined tRNA halves, e.g. of tRNAGlu, tRNAGln, and tRNALys, and removes an intervening sequence element from the tRNA precursor, cleavage pattern determination, overview | Nanoarchaeum equitans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | heteromeric endonuclease type | Nanoarchaeum equitans |
Synonyms | Comment | Organism |
---|---|---|
splicing endonuclease | - |
Nanoarchaeum equitans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Nanoarchaeum equitans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nanoarchaeum equitans |