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4.6.1.16: tRNA-intron lyase

This is an abbreviated version!
For detailed information about tRNA-intron lyase, go to the full flat file.

Word Map on EC 4.6.1.16

Reaction

pretRNA
=
a 3'-half-tRNA molecule with a 5'-OH end
 +
a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
 +
an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus

Synonyms

AF endonuclease, AFN, AF_0900, archaeal pre-tRNA splicing endonuclease, ARMAN-2 EndA, EC 3.1.27.9, EndA, EndATa, epsilon2 endonuclease, Gamma-toxin, hClp1, HEAB, homing endonuclease, HSPC117, I-TevI, intron-encoded endonuclease, MJ1424, More, nuclease, transfer ribonucleate intron endoribo-, pre-tRNA splicing endonuclease, RNA splicing endonuclease, RNA-splicing endonuclease, Sen2, splicing endonuclease, ssol_1416, STK_03580, three-unit tRNA splicing endonuclease, transfer ribonucleate intron endoribonuclease, transfer splicing endonuclease, tRNA anticodon nucleases (ACNs), tRNA endonuclease, tRNA intron endonuclease, tRNA intron-splicing endonuclease, tRNA SEN, tRNA splicing endonuclease, tRNA splicing ligase, tRNA-intron endonuclease, tRNA-splicing endonuclease, tRNATRPintron endonuclease

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.16 tRNA-intron lyase

Molecular Weight

Molecular Weight on EC 4.6.1.16 - tRNA-intron lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease
146000
-
deduced from gene sequences of subunits
15000
-
alphabetagammadelta, 1 * 54000, 1 * 44000, 1* 34000, 1 * 15000, SDS-PAGE
29500
-
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease
37000
-
2 * 37000, SDS-PAGE
40700
-
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease
44000
-
alphabetagammadelta, 1 * 54000, 1 * 44000, 1* 34000, 1 * 15000, SDS-PAGE
52500
-
x * 100000, polypeptide A, + y * 52500, polypeptide B, + z * 40700, polypeptide C, + v * 29500, polypeptide D, + 4 minor polypeptides of MW 140000, 120000, 90000 and 70000 are detected in SDS-PAGE of most highly purified fraction, probably polypeptides B, C, and D exist as a trimeric complex and represent the tRNA splicing endonuclease
54000
-
alphabetagammadelta, 1 * 54000, 1 * 44000, 1* 34000, 1 * 15000, SDS-PAGE