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4.6.1.16: tRNA-intron lyase

This is an abbreviated version!
For detailed information about tRNA-intron lyase, go to the full flat file.

Word Map on EC 4.6.1.16

Reaction

pretRNA
=
a 3'-half-tRNA molecule with a 5'-OH end
 +
a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
 +
an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus

Synonyms

AF endonuclease, AFN, AF_0900, archaeal pre-tRNA splicing endonuclease, ARMAN-2 EndA, EC 3.1.27.9, EndA, EndATa, epsilon2 endonuclease, Gamma-toxin, hClp1, HEAB, homing endonuclease, HSPC117, I-TevI, intron-encoded endonuclease, MJ1424, More, nuclease, transfer ribonucleate intron endoribo-, pre-tRNA splicing endonuclease, RNA splicing endonuclease, RNA-splicing endonuclease, Sen2, splicing endonuclease, ssol_1416, STK_03580, three-unit tRNA splicing endonuclease, transfer ribonucleate intron endoribonuclease, transfer splicing endonuclease, tRNA anticodon nucleases (ACNs), tRNA endonuclease, tRNA intron endonuclease, tRNA intron-splicing endonuclease, tRNA SEN, tRNA splicing endonuclease, tRNA splicing ligase, tRNA-intron endonuclease, tRNA-splicing endonuclease, tRNATRPintron endonuclease

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.16 tRNA-intron lyase

Purification

Purification on EC 4.6.1.16 - tRNA-intron lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
chromatographic methods
-
copurification of recombinant His-tagged SULSO alpha-subunit and untagged beta-subunit from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
partial, strain M304
-
recombinant EndA from Escherichia coli strain Rosetta 2(DE3) by heat treatment at 70°C for 30 min, followed by heparin affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged subunits by nickel affinity chromatography
-
recombinant truncated fully active dimeric enzyme form lacking the N-terminal domain from Escherichia coli strain BLR(DE3) to homogeneity
-
recombinant wild-type and mutant EndAs from Escherichia coli strain Rosetta 2(DE3) by heat treatment at 70°C for 30 min, followed by heparin affinity chromatography and gel filtration
recombinant wild-type and mutant EndAs from Escherichia coli strain Rosetta 2(DE3) by heat treatment at 70°C for 30 min, followed by metal affinity chromatography and gel filtration
TALON metal affinity resin column chromatography