4.4.1.17: Holocytochrome-c synthase
This is an abbreviated version!
For detailed information about Holocytochrome-c synthase, go to the full flat file.
Word Map on EC 4.4.1.17
Reaction
Synonyms
CCHL, ccsA1, cyc2p, CYC3, cytochrome c heme lyase, Cytochrome c heme-lyase, Cytochrome c synthase, HCC synthase, HCCH, HCCS, heme lyase, holocytochrome c synthase, Holocytochrome c synthetase, Holocytochrome c-type synthase, Holocytochrome-C synthase, Holocytochrome-C-type synthase, Synthetase, holocytochrome c, System III
ECTree
4.4.1.17 Holocytochrome-c synthaseAdvanced search results
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results (Cloned
Cloned on EC 4.4.1.17 - Holocytochrome-c synthase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
cloning in Escherichia coli
coexpression of N-terminally GST-tagged wild-type enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants in Escherichia coli
coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3)
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expression in Escherichia coli
expression of the enzyme in Escherichia coli cytosplasm, coexpression with several mutant variants of holocytochrome c552 from Hydrogenobacter thermophilus, the latter are expressed in the periplasm, or several cytochrome c variants from Equus caballus
heterologous expression in a multicopy yeast vector
recombinant coexpression of GST-tagged wild-type and mutant enzymes with cytochrome c in Escherichia coli strain RK103 membranes
recombinant coexpression of the GST-tagged enzyme with cytochrome c mutants, containing individual cysteine, histidine, double cysteine, and triple cysteine/histidine substitutions of conserved motif CXXCH, in Escherichia coli strain RK103 membranes, single and double mutants form a complex with the enzyme but not the triple mutant
recombinant expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3) cytoplasm, coexpression with Equus caballus cytochrome c
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