Cloned (Comment) | Organism |
---|---|
coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C15A | site-directed mutagenesis, cytoplasm-targeted mutant, the mutant is matured and undergoes heme attachment like the wild-type enzyme | Saccharomyces cerevisiae |
C18A | site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment | Saccharomyces cerevisiae |
F15A | site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c | Saccharomyces cerevisiae |
F15E | site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c | Saccharomyces cerevisiae |
F15I | site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme | Saccharomyces cerevisiae |
F15W | site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme | Saccharomyces cerevisiae |
F15Y | site-directed mutagenesis, periplasm-targeted mutant, the mutant shows unaltered holocytochrome c production like the wild-type enzyme | Saccharomyces cerevisiae |
H19K | site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment | Saccharomyces cerevisiae |
H19M | site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment | Saccharomyces cerevisiae |
H19R | site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment | Saccharomyces cerevisiae |
additional information | construction of T17, K16, G29, H45, and K60 deletion mutants and of a mutant with periplasmic targeting sequence | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Apocytochrome c + heme | Saccharomyces cerevisiae | - |
Holocytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Apocytochrome c + heme | - |
Saccharomyces cerevisiae | Holocytochrome c | - |
? | |
Apocytochrome c + heme | Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiae cytochrome c are coexpressed in Escherichia coli strain BL21(DE3) with Saccharomyces cerevisiae holocytochrome c synthase. No product synthesis from truncated cytochrome c1 mutants G29X, H45X and K60X, with the X indicating the position of the inserted stop codon | Saccharomyces cerevisiae | Holocytochrome c | - |
? | |
additional information | the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important protein-protein recognition motifs in enzyme-substrate interaction, the N-terminal region of the mitochondrial cytochrome c sequence is critical for attachment of heme to apocytochrome c | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HCC synthase | - |
Saccharomyces cerevisiae |
HCCS | - |
Saccharomyces cerevisiae |
holocytochrome c synthase | - |
Saccharomyces cerevisiae |