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Literature summary for 4.4.1.17 extracted from

  • Babbitt, S.E.; San Francisco, B.; Mendez, D.L.; Lukat-Rodgers, G.S.; Rodgers, K.R.; Bretsnyder, E.C.; Kranz, R.G.
    Mechanisms of mitochondrial holocytochrome c synthase and the key roles played by cysteines and histidine of the heme attachment site, CysXXCysHis (2014), J. Biol. Chem., 289, 28795-18807.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.17

Cloned(Commentary)

Cloned (Comment) Organism
recombinant coexpression of the GST-tagged enzyme with cytochrome c mutants, containing individual cysteine, histidine, double cysteine, and triple cysteine/histidine substitutions of conserved motif CXXCH, in Escherichia coli strain RK103 membranes, single and double mutants form a complex with the enzyme but not the triple mutant Homo sapiens

Protein Variants

Protein Variants Comment Organism
C15S the HCCS:cyt c C15S and C18A mutants support the formation of single thioether cross-links between the heme and cyt c at the vinyl position alternate to the mutation Homo sapiens
C18A the HCCS:cyt c C15S and C18A mutants support the formation of single thioether cross-links between the heme and cyt c at the vinyl position alternate to the mutation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Apocytochrome c + heme Homo sapiens
-
 Holocytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P53701
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme and cytochrome c mutants from Escherichia coli strain RK103 membranes by ultracentrifugation, glutahione affinity chromatographyand ultrafiltration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Apocytochrome c + heme
-
 Homo sapiens Holocytochrome c
-
 ?
Apocytochrome c + heme the enzyme attaches heme to wild-type cytochrome, and to mutant cytochromes containing individual cysteine, histidine, and double cysteine of conserved motif CXXCH, but not the mutant with triple cysteine/histidine substitutions, overview. His19 in cytochrome c is important for 1. to provide the second axial ligand to the heme iron in preparation for covalent attachment, 2. to spatially position the two cysteinyl sulfurs adjacent to the two heme vinyl groups for thioether formation, and 3. to aid in release of the holocytochrome c from the enzyme's active site. Substitutions of His19 in cytochrome c to seven other residues (G,A,M,R,K,C,Y) show that only mutant H19M is able to carry out these three roles, albeit at lower efficiencies than the wild-type His19. The histidine in the CXXCH motif acts as an axial ligand to the heme iron Homo sapiens Holocytochrome c
-
 ?

Synonyms

Synonyms Comment Organism
HCCS
-
 Homo sapiens
holocytochrome c synthase
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function for mitochondrial cytochrome c assembly, the enzyme binds heme and apocytochrome c substrate to catalyze a covalent attachment of heme by thioether bonds between heme vinyl groups and a conserved CXXCH motif of cytochrome c/c1, and subsequently releases holocytochrome c for proper folding to its native structure, mechanism of assembly, overview Homo sapiens