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4.4.1.17: Holocytochrome-c synthase

This is an abbreviated version!
For detailed information about Holocytochrome-c synthase, go to the full flat file.

Word Map on EC 4.4.1.17

Reaction

Holocytochrome c
=
apocytochrome c
 +
heme

Synonyms

CCHL, ccsA1, cyc2p, CYC3, cytochrome c heme lyase, Cytochrome c heme-lyase, Cytochrome c synthase, HCC synthase, HCCH, HCCS, heme lyase, holocytochrome c synthase, Holocytochrome c synthetase, Holocytochrome c-type synthase, Holocytochrome-C synthase, Holocytochrome-C-type synthase, Synthetase, holocytochrome c, System III

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.17 Holocytochrome-c synthase

Purification

Purification on EC 4.4.1.17 - Holocytochrome-c synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, solubilization by Triton X-100
-
partial, subfractionation of mitochondria with digitonin
-
recombinant GST-tagged enzyme and cytochrome c mutants from Escherichia coli strain RK103 membranes by ultracentrifugation, glutahione affinity chromatographyand ultrafiltration
recombinant GST-tagged wild-type and mutant enzymes and cytochrome c from Escherichia coli strain RK103 membranes by ultracentrifugation, glutahione affinity chromatographyand ultrafiltration
wild-type membrane-bound human GST-tagged enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants from Escherichia coli