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4.4.1.13: cysteine-S-conjugate beta-lyase

This is an abbreviated version!
For detailed information about cysteine-S-conjugate beta-lyase, go to the full flat file.

Word Map on EC 4.4.1.13

Reaction

2-aminoprop-2-enoate
=
2-iminopropanoate

Synonyms

AAR, beta-cystathionase, beta-lyase, C-DES, C-S lyase, CBL, CBL/ALR, CBS, CCBL2, Ctl1, Ctl2, cystathionine beta-lyase, cystathionine beta/gamma-lyase, cystathionine lyase, cysteine conjugate beta-lyase, cysteine conjugate. beta.-lyase, cysteine lyase, cysteine S-conjugate beta-lyase, cysteine-S-conjugate beta-lyase, cystine C-S lyase, cystine lyase, EC 4.4.1.6, EC 4.4.1.8, EcCBL, glutamine transaminase K, glutamine transaminase K/cysteine conjugate beta-lyase, GTK, Irc7p, KAT, KAT III, KYAT3, kynurenine aminotransferase, kynurenine-oxoglutarate transaminase 3, L-cystine C-S lyase, LACBS_00576, lyase, cystathionine beta-, lyase, cysteine conjugate.beta., MalY, MetC, More, Mp CBL, ORF5, osteotoxin, patB, PatB protein, Str3p, TmCBL, TTHA1620, wMelCBL

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.13 cysteine-S-conjugate beta-lyase

General Stability

General Stability on EC 4.4.1.13 - cysteine-S-conjugate beta-lyase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of 15% glycerol extends the enzymatic lifetime to several days and permitted freezing of the preparation at -20°C
-
inactivated by repeated freezing and thawing
-
no decrease in activity after repeated freezing, -20°C, and thawing
-
no loss of activity after repeated thawing and freezing
-
not stable to lyophilization
-
pyridoxal 5'-phosphate stabilizes
rapid loss of activity during dialysis
-
repeated freezing, -20°C, and thawing has no effect
-
the buffer exchange conditions to stabilize the isolated protein and maintain its enzymatic activity include 500 mM KCl and 20 mM EDTA. By including 10% (w/v) glycerol in the buffer, less than 6% of the activity is lost in a freeze-thaw cycle, compared to the 56% loss in the presence of 10 mM imidazole
the coenzyme strongly increases the protein stability. It is essential for the step involving dissociation of dimer into monomers and is not required for refolding of single monomers, but it is necessary to attain the native dimeric structure critical for the biological activities of MalY
-