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4.4.1.13: cysteine-S-conjugate beta-lyase

This is an abbreviated version!
For detailed information about cysteine-S-conjugate beta-lyase, go to the full flat file.

Word Map on EC 4.4.1.13

Reaction

2-aminoprop-2-enoate
=
2-iminopropanoate

Synonyms

AAR, beta-cystathionase, beta-lyase, C-DES, C-S lyase, CBL, CBL/ALR, CBS, CCBL2, Ctl1, Ctl2, cystathionine beta-lyase, cystathionine beta/gamma-lyase, cystathionine lyase, cysteine conjugate beta-lyase, cysteine conjugate. beta.-lyase, cysteine lyase, cysteine S-conjugate beta-lyase, cysteine-S-conjugate beta-lyase, cystine C-S lyase, cystine lyase, EC 4.4.1.6, EC 4.4.1.8, EcCBL, glutamine transaminase K, glutamine transaminase K/cysteine conjugate beta-lyase, GTK, Irc7p, KAT, KAT III, KYAT3, kynurenine aminotransferase, kynurenine-oxoglutarate transaminase 3, L-cystine C-S lyase, LACBS_00576, lyase, cystathionine beta-, lyase, cysteine conjugate.beta., MalY, MetC, More, Mp CBL, ORF5, osteotoxin, patB, PatB protein, Str3p, TmCBL, TTHA1620, wMelCBL

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.13 cysteine-S-conjugate beta-lyase

Cloned

Cloned on EC 4.4.1.13 - cysteine-S-conjugate beta-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of an overproducing strain of Escherichia coli
-
DNA and amino acid sequence determination and analysis, expression of His-tagged MalY in Escherichia coli
DNA and amino acid sequence determination and analysis, expression of His-tagged MetC in Escherichia coli
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in a baculovirus/insect cell protein expression system
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expressed in Escherichia coli BL21(DE3) cells
expressed in Saccharomyces cerevisiae strain VIN 13 and in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli. Overexpression of the enzyme complements the methionine auxotrophy of an Escherichia coli metC mutant
-
expression of wild-type and chimeric mutant enzymes in Escherichia coli strain ER1821, in vivo complementation of methionine-auxotrophic Escherichia coli strains, lacking the genes encoding cystathionine gamma-synthase and cystathionine beta-lyase, with chimeric mutants
from strain MG1363 and from strain B78
gene malY, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene met-2+, DNA and amino acid sequence determination and analysis, presence of CYS3 activator binding sites on the met-2+ promoter, quantitative enzyme expression analysis
gene metC or SH2636, phylogenetic tree and analysis, cloning from strains AX3 and CSC1435, expression of His-tagged enzyme in Escherichia coli strain BL21
-
gene metC, phylogenetic analysis, the recombinant His6-tagged enzyme TmCBL is overexpressed in a metC-deficient DELTAmetC Escherichia coli mutant strain and can complement it. The enzyme rescues the alanine racemase knockout, Escherichia coli strain MB2795 (DELTAalrDELTAdadX), as quickly as expressing Escherichia coli ALR itself. Recombinant production of His6-tagged enzyme in a soluble form
gene metC, phylogenetic analysis, the recombinant His6-tagged enzyme wMelCBL is overexpressed in a metC-deficient DELTAmetC Escherichia coli mutant strain and can complement it. The enzyme rescues the alanine racemase knockout, Escherichia coli strain MB2795 (DELTAalrDELTAdadX), as quickly as expressing Escherichia coli ALR itself. Recombinant production of His6-tagged enzyme in a soluble form
gene metC, phylogenetic analysis, the recombinant MBP-tagged enzyme PuCBL is overexpressed in a metC-deficient DELTAmetC Escherichia coli mutant strain and can complement it partially. The enzyme rescues the alanine racemase knockout, Escherichia coli strain MB2795 (DELTAalrDELTAdadX), as quickly as expressing Escherichia coli ALR itself. Attempts to optimize recombinant production of the Pelagibacter ubique enzyme (PuCBL) in a soluble form are unsuccessful
gene metC, phylogenetic analysis. The recombinant enzyme EcCBL is overexpressed in a metC-deficient DELTAmetC Escherichia coli mutant strain and can complement it partially
mutant enzymes are expressed in Escherichia coli KS1000 cells
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mutant enzymes are expressed in the Escherichia coli KS1000 metC:cat strain
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overexpression in Escherichia coli
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overexpression in Escherichia coli CAG18475
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overexpression of cysteine-S-conjugate beta-lyase gene from Escherichia coli in a commercial wine yeast strain
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recloning of the gene from a transducing phage lambda carrying the structural gene for beta-cystathionase
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain ER1821 metC::cat
the deduced amino acid sequence of beta-cystathionase shows extensive homology with that of cystathionine gamma-synthase
-