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4.2.2.20: chondroitin-sulfate-ABC endolyase

This is an abbreviated version!
For detailed information about chondroitin-sulfate-ABC endolyase, go to the full flat file.

Word Map on EC 4.2.2.20

Reaction

R1-[beta-D-GalNAc-1,4-L-iduronic acid]-R2
=
R1-[beta-D-GalNAc]
 +
[4-deoxy-L-idu-4-enuronic acid]-R2

Synonyms

Ch'ase, Ch'ase ABC, chABC, chondroitinase ABC, chondroitinase ABC I, chondroitinase AC, ChS ABC lyase I, EC 4.2.2.4, EC 4.2.99.6

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.20 chondroitin-sulfate-ABC endolyase

Temperature Stability

Temperature Stability on EC 4.2.2.20 - chondroitin-sulfate-ABC endolyase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
210 min, 78% residual activity
40
-
30 min, 47% loss of activity
47
melting temperature
49
melting temperature, presence of 20% glycerol
52
melting temperature, presence of 1 M sorbitol
54
melting temperature, presence of 1 M trehalose
additional information
-
in commercial preparations, there is a significant effect of lot and time on thermal stability. Average enzymatic activity is significantly decreased after 1 and 3 days at 39°C and 37°C, resp. The average activity seen after 1 day is significantly different between the two temperatures. Addition of bovine serum albumin preserves enzymatic activity at 1 day, but not 3 days, at 39°C