4.2.2.20: chondroitin-sulfate-ABC endolyase
This is an abbreviated version!
For detailed information about chondroitin-sulfate-ABC endolyase, go to the full flat file.
Word Map on EC 4.2.2.20
-
4.2.2.20
-
proteoglycans
-
glycosaminoglycans
-
heparan
-
dermatan
-
collagen
-
cartilage
-
hyaluronidase
-
cord
-
hyaluronic
-
heparitinase
-
cspgs
-
articular
-
4-sulfate
-
scar
-
nitrous
-
35ssulfate
-
decorin
-
alcian
-
aggrecan
-
keratanase
-
perineuronal
-
intervertebral
-
biglycan
-
iduronic
-
endo-beta-galactosidase
-
chemonucleolysis
-
monosulfated
-
chondroitin-4-sulfate
-
biotechnology
-
intradiscal
-
cetylpyridinium
-
neurocan
-
molecular biology
-
versican
-
chymopapain
-
fibrosus
-
sulfate-containing
-
hexasaccharide
-
unsulfated
-
medicine
-
6-sulphated
-
hemisection
-
wisteria
-
oversulfated
-
hexuronic
-
pulposus
-
stub
-
beta-d-xyloside
-
3hglucosamine
-
brevican
-
undersulfated
-
phosphacan
- 4.2.2.20
- proteoglycans
- glycosaminoglycans
- heparan
- dermatan
- collagen
- cartilage
- hyaluronidase
- cord
-
hyaluronic
- heparitinase
-
cspgs
-
articular
- 4-sulfate
- scar
-
nitrous
-
35ssulfate
- decorin
-
alcian
- aggrecan
- keratanase
-
perineuronal
-
intervertebral
- biglycan
-
iduronic
- endo-beta-galactosidase
-
chemonucleolysis
-
monosulfated
- chondroitin-4-sulfate
- biotechnology
-
intradiscal
-
cetylpyridinium
- neurocan
- molecular biology
- versican
- chymopapain
-
fibrosus
-
sulfate-containing
- hexasaccharide
-
unsulfated
- medicine
-
6-sulphated
-
hemisection
- wisteria
-
oversulfated
-
hexuronic
- pulposus
-
stub
-
beta-d-xyloside
-
3hglucosamine
- brevican
-
undersulfated
- phosphacan
Reaction
Synonyms
Ch'ase, Ch'ase ABC, chABC, chondroitinase ABC, chondroitinase ABC I, chondroitinase AC, ChS ABC lyase I, EC 4.2.2.4, EC 4.2.99.6
ECTree
Advanced search results
Engineering
Engineering on EC 4.2.2.20 - chondroitin-sulfate-ABC endolyase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Q140A
mutation improves both activity and thermal stability of the enzyme, and additionally improves resistance to trypsin degradation
Q140G
mutation improves both activity and thermal stability of the enzyme, and additionally improves resistance to trypsin degradation
additional information
engineering of a mammalian expression system of an epitope-tagged chondroitinase ABC protein. At the 5'-end, an optimized KOZAK-ATG sequence is inserted, to allow efficient eukaryotic translation, followed by a 60 base pair secretion sequence from the fish Oreochromis aureus, engineered for efficient crosshost recombinant protein expression. At the 3'-end, a 3xFlag epitope tag is added. The addition of the eukaryotic secretion signal sequence allows secretion, but interferes with function of the secreted enzyme. In contrast, expression of the chondroitinase ABC gene without N-terminal eukaryotic secretion sequence or bacterial hydrophobic leader sequence leads to efficient secretion of a biologically active chondroitinase ABC protein from both immortalized and primary cells. The C-terminal epitope tag can be utilized to follow expression of this protein
additional information
-
engineering of a mammalian expression system of an epitope-tagged chondroitinase ABC protein. At the 5'-end, an optimized KOZAK-ATG sequence is inserted, to allow efficient eukaryotic translation, followed by a 60 base pair secretion sequence from the fish Oreochromis aureus, engineered for efficient crosshost recombinant protein expression. At the 3'-end, a 3xFlag epitope tag is added. The addition of the eukaryotic secretion signal sequence allows secretion, but interferes with function of the secreted enzyme. In contrast, expression of the chondroitinase ABC gene without N-terminal eukaryotic secretion sequence or bacterial hydrophobic leader sequence leads to efficient secretion of a biologically active chondroitinase ABC protein from both immortalized and primary cells. The C-terminal epitope tag can be utilized to follow expression of this protein