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Literature summary for 4.2.2.20 extracted from

  • Nazari-Robati, M.; Khajeh, K.; Aminian, M.; Fathi-Roudsari, M.; Golestani, A.
    Co-solvent mediated thermal stabilization of chondroitinase ABC I form Proteus vulgaris (2012), Int. J. Biol. Macromol., 50, 487-492.
    View publication on PubMed
Search for more literature for 4.2.2.20

General Stability

General Stability Organism
the enzyme catalytic activity and intrinsic fluorescence intensity increase in the presence of cosolvents glycerol, sorbitol and trehalose whereas no considerable conformational changes are observed in far-UV CD spectra. In the presence of trehalose a significant increase in meltin temperature is observed. Proteus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 chondroitin 4-sulfate presence of 1 M sorbitol, pH 6.8, 37°C Proteus vulgaris
0.035
-
 chondroitin 4-sulfate presence of 1 M trehalose, pH 6.8, 37°C Proteus vulgaris
0.039
-
 chondroitin 4-sulfate presence of 20% glycerol, pH 6.8, 37°C Proteus vulgaris
0.044
-
 chondroitin 4-sulfate pH 6.8, 37°C Proteus vulgaris
0.055
-
 chondroitin 6-sulfate pH 6.8, 37°C Proteus vulgaris
0.094
-
 dermatan sulfate pH 6.8, 37°C Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris P59807
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
-18°C, 3 days, complete loss of activity Proteus vulgaris
25°C, 17 days, complete loss of activity, in presence of 1 M trehalose 85% residual activity after 21 days Proteus vulgaris
37°C, 10 days, presence of 1 M trehalose, 20% residual activity Proteus vulgaris
4°C, 21 days, 80% residual activity Proteus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 4-sulfate
-
 Proteus vulgaris ?
-
 ?
chondroitin 6-sulfate
-
 Proteus vulgaris ?
-
 ?
dermatan sulfate
-
 Proteus vulgaris ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Proteus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
 melting temperature Proteus vulgaris
49
-
 melting temperature, presence of 20% glycerol Proteus vulgaris
52
-
 melting temperature, presence of 1 M sorbitol Proteus vulgaris
54
-
 melting temperature, presence of 1 M trehalose Proteus vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
48.5
-
 dermatan sulfate pH 6.8, 37°C Proteus vulgaris
69.7
-
 chondroitin 4-sulfate presence of 1 M sorbitol, pH 6.8, 37°C Proteus vulgaris
75.8
-
 chondroitin 4-sulfate presence of 1 M trehalose, pH 6.8, 37°C Proteus vulgaris
78.8
-
 chondroitin 4-sulfate presence of 20% glycerol, pH 6.8, 37°C Proteus vulgaris
84.8
-
 chondroitin 4-sulfate pH 6.8, 37°C Proteus vulgaris
100
-
 chondroitin 6-sulfate pH 6.8, 37°C Proteus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Proteus vulgaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1517
-
 dermatan sulfate pH 6.8, 37°C Proteus vulgaris
1800
-
 chondroitin 6-sulfate pH 6.8, 37°C Proteus vulgaris
1933
-
 chondroitin 4-sulfate pH 6.8, 37°C Proteus vulgaris
2017
-
 chondroitin 4-sulfate presence of 20% glycerol, pH 6.8, 37°C Proteus vulgaris
2117
-
 chondroitin 4-sulfate presence of 1 M sorbitol, pH 6.8, 37°C Proteus vulgaris
2167
-
 chondroitin 4-sulfate presence of 1 M trehalose, pH 6.8, 37°C Proteus vulgaris