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4.2.2.20: chondroitin-sulfate-ABC endolyase

This is an abbreviated version!
For detailed information about chondroitin-sulfate-ABC endolyase, go to the full flat file.

Word Map on EC 4.2.2.20

Reaction

R1-[beta-D-GalNAc-1,4-L-iduronic acid]-R2
=
R1-[beta-D-GalNAc]
 +
[4-deoxy-L-idu-4-enuronic acid]-R2

Synonyms

Ch'ase, Ch'ase ABC, chABC, chondroitinase ABC, chondroitinase ABC I, chondroitinase AC, ChS ABC lyase I, EC 4.2.2.4, EC 4.2.99.6

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.20 chondroitin-sulfate-ABC endolyase

Expression

Expression on EC 4.2.2.20 - chondroitin-sulfate-ABC endolyase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
when expressed in a mammalian cell, residues N675, N515, N345, N338 and N282 are all glycosylated which prevents secretion of functional enzyme. Directed mutagenesis of selected N-glycosylation sites allows efficient secretion of active chondroitinase. Mutation if residue N751 renders the enzyme inactive. Mutations required for efficient secretion of active enzyme are those eliminating glycosylation at N282, N336, N345, and N515, and mutation of N675 increases the activity still further
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