4.2.1.114: methanogen homoaconitase

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For detailed information about methanogen homoaconitase, go to the full flat file.

Reaction

Synonyms

CNAG_02565, HacA, HACN, Homoaconitase, LYS4, methanogen HACN, MJ1003, MJ1271, More

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.114 methanogen homoaconitase

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Results	in table
307	AA Sequence
2	Application
4	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
2	Expression
4	General Information
6	Inhibitors
20	KM Value [mM]
2	Localization
4	Metals/Ions
9	Natural Substrates/ Products (Substrates)
7	Organism
7	Pathway
1	PDB ID
2	pH Optimum
6	Protein Variants
3	Purification (Commentary)
3	Reaction
7	Reference
24	Substrates and Products (Substrate)
1	Subunits
19	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
19	Turnover Number [1/s]

Purification

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Purification on EC 4.2.1.114 - methanogen homoaconitase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Methanocaldococcus jannaschii

Q58409

726596

recombinant MJ1003 and MJ1271 proteins from Escherichia coli strain BL21(DE3) by heat treatment of the cell lysate, followed by anion exchange chromatography, dialysis, and ultrafiltration

Methanocaldococcus jannaschii

Q58991

693150

Toyopearl SuperQ-650 M column chromatography, Resource S column chromatography, and Superdex 200 gel filtration

Methanocaldococcus jannaschii

-

702368