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4.2.1.114: methanogen homoaconitase

This is an abbreviated version!
For detailed information about methanogen homoaconitase, go to the full flat file.

Reaction

(R)-2-hydroxybutane-1,2,4-tricarboxylate
=
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate

Synonyms

CNAG_02565, HacA, HACN, Homoaconitase, LYS4, methanogen HACN, MJ1003, MJ1271, More

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.114 methanogen homoaconitase

KM Value

KM Value on EC 4.2.1.114 - methanogen homoaconitase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036 - 0.66
(1E)-hex-1-ene-1,2,6-tricarboxylic acid
0.03 - 1.6
(1E)-pent-1-ene-1,2,5-tricarboxylic acid
1.5
(R)-homocitrate
pH 8.5, 60°C, recombinant enzyme, dehydratase reaction
0.022 - 0.46
but-1-ene-1,2,4-tricarboxylic acid
0.03
cis-homo2aconitate
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction
0.036
cis-homo3aconitate
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction
0.175
cis-homo4aconitate
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction
0.022
cis-homoaconitate
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction
0.33
Maleate
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction
additional information
additional information
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