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4.2.1.114: methanogen homoaconitase

This is an abbreviated version!
For detailed information about methanogen homoaconitase, go to the full flat file.

Reaction

(R)-2-hydroxybutane-1,2,4-tricarboxylate
=
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate

Synonyms

CNAG_02565, HacA, HACN, Homoaconitase, LYS4, methanogen HACN, MJ1003, MJ1271, More

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.114 methanogen homoaconitase

Engineering

Engineering on EC 4.2.1.114 - methanogen homoaconitase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R26K
-
the variant forms an relatively efficient isopropylmalate isomerase enzyme
R26V
-
the variant forms an relatively efficient isopropylmalate isomerase enzyme
R26V/T27Y
-
the variant resembles the MJ1277 isopropylmalate isomerase small subunit in its flexible loop sequence but demonstrates the broad substrate specificity of the R26V variant
T27A t
-
the variant has uniformly lower specificity constants for both isopropylmalate isomerase and methanogen homoaconitase substrates
additional information