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4.1.1.B12: lysine/ornithine carboxy-lyase

This is an abbreviated version!
For detailed information about lysine/ornithine carboxy-lyase, go to the full flat file.

Word Map on EC 4.1.1.B12

Reaction

L-lysine
=
cadaverine
+
CO2

Synonyms

arginine/ornithine decarboxylase, HsLDC, L-lysine decarboxylase, L/ODC, LcL/ODC, LDC, LDC/ODC, Lys/Orn decarboxylase, lysine/ornithine decarboxylase, More, OAD, OAD1, SrLDC, vvLDC

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.B12 lysine/ornithine carboxy-lyase

Cloned

Cloned on EC 4.1.1.B12 - lysine/ornithine carboxy-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene ldc, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged LDC/ODC in Escherichia coli
gene srLDC, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene vvLDC, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
six cDNAs encoding lysine decarboxylases (LDCs) are cloned from Huperzia serrata by degenerate PCR and rapid amplification of cDNA ends (RACE), phylogenetic analysis, one HsLDC isoform is functionally characterized as lysine decarboxylase. Recombinant transient expression of HsLDC in Nicotiana tabacum leaves specifically increase cadaverine content from zero to 0.75 mg per gram of dry mass. Recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-