Information on EC 4.1.1.B12 - lysine/ornithine carboxy-lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.1.B12
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
lysine/ornithine carboxy-lyase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-lysine = cadaverine + CO2
show the reaction diagram
-
-
-
-
L-ornithine = putrescine + CO2
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
L-lysine/L-ornithine carboxy-lyase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
transgenic tobacco suspension cells and hairy roots expressing lysine decarboxylase produce enhanced levels of cadaverine-derived alkaloids, and transgenic Arabidopsis plants produce enhanced levels of cadaverine, indicating the involvement of this enzyme in lysine decarboxylation to form cadaverine. Enzyme is involved in the decarboxylation of L-Lys, which is the first step of quinolizidine alkaloid biosynthesis in quinolizidine alkaloid-producing plants
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-lysine
cadaverine + CO2
show the reaction diagram
L-ornithine
putrescine + CO2
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-difluoromethylornithine
inhibitor of ornithine decarboxylase, inhibitory
L-ornithine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1 - 97.8
L-lysine
1.04 - 1.59
L-ornithine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.18 - 143
L-lysine
0.29 - 2.3
L-ornithine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.469 - 1558
L-lysine
134
0.454 - 1496
L-ornithine
192
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
in bitter cultivar, highest expression in young leaf
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
x * 47000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F344H
mutation in conserved amino acid, 41fold increase in Km value for L-lysine, 1.5fold for L-ornithine
F344Y
mutation in conserved amino acid, 2.2fold increase in Km value for L-lysine, 1.5fold for L-ornithine
M341L
mutation in conserved amino acid, minor changes in Km values