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Literature summary for 4.1.1.B12 extracted from

  • Bunsupa, S.; Katayama, K.; Ikeura, E.; Oikawa, A.; Toyooka, K.; Saito, K.; Yamazaki, M.
    Lysine decarboxylase catalyzes the first step of quinolizidine alkaloid biosynthesis and coevolved with alkaloid production in leguminosae (2012), Plant Cell, 24, 1202-1216.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.B12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lupinus angustifolius
expression in Escherichia coli Sophora flavescens
expression in Escherichia coli Echinosophora koreensis

Protein Variants

Protein Variants Comment Organism
F344H mutation in conserved amino acid, 41fold increase in Km value for L-lysine, 1.5fold for L-ornithine Lupinus angustifolius
F344Y mutation in conserved amino acid, 2.2fold increase in Km value for L-lysine, 1.5fold for L-ornithine Lupinus angustifolius
M341L mutation in conserved amino acid, minor changes in Km values Lupinus angustifolius

Inhibitors

Inhibitors Comment Organism Structure
alpha-difluoromethylornithine inhibitor of ornithine decarboxylase, inhibitory Lupinus angustifolius
L-ornithine competitive Echinosophora koreensis
L-ornithine competitive Lupinus angustifolius
L-ornithine competitive Sophora flavescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.04
-
 L-ornithine mutant M341L, pH 7.5, 37°C Lupinus angustifolius
1.05
-
 L-ornithine wild-type, pH 7.5, 37°C Lupinus angustifolius
1.32
-
 L-ornithine pH 7.5, 37°C Echinosophora koreensis
1.53
-
 L-ornithine pH 7.5, 37°C Sophora flavescens
1.53
-
 L-ornithine mutant F344H, pH 7.5, 37°C Lupinus angustifolius
1.59
-
 L-ornithine mutant F344Y, pH 7.5, 37°C Lupinus angustifolius
2.1
-
 L-lysine pH 7.5, 37°C Sophora flavescens
2.37
-
 L-lysine wild-type, pH 7.5, 37°C Lupinus angustifolius
2.49
-
 L-lysine mutant M341L, pH 7.5, 37°C Lupinus angustifolius
3.85
-
 L-lysine pH 7.5, 37°C Echinosophora koreensis
5.26
-
 L-lysine mutant F344Y, pH 7.5, 37°C Lupinus angustifolius
97.8
-
 L-lysine mutant F344H, pH 7.5, 37°C Lupinus angustifolius

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Lupinus angustifolius 9536
-
plastid
-
 Sophora flavescens 9536
-
plastid
-
 Echinosophora koreensis 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, SDS-PAGE Lupinus angustifolius
49000
-
 x * 49000, SDS-PAGE Sophora flavescens
49000
-
 x * 49000, SDS-PAGE Echinosophora koreensis

Organism

Organism UniProt Comment Textmining
Echinosophora koreensis F5HPS1
-
-
Lupinus angustifolius F5HPR7
-
-
Sophora flavescens F5HPS0
-
-

Reaction

Reaction Comment Organism Reaction ID
L-ornithine = putrescine + CO2 decarboxylation of L-ornithine and L-lysine is catalyzed with similar efficiency Lupinus angustifolius
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf in bitter cultivar, highest expression in young leaf Lupinus angustifolius
-
additional information no detectable expression in sweet cultivar Lupinus angustifolius
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 0.5 in wild type Lupinus angustifolius cadaverine + CO2
-
 ?
L-lysine the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.03 Echinosophora koreensis cadaverine + CO2
-
 ?
L-lysine the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.47 Sophora flavescens cadaverine + CO2
-
 ?
L-ornithine the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 0.5 in wild type Lupinus angustifolius putrescine + CO2
-
 ?
L-ornithine the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.03 Echinosophora koreensis putrescine + CO2
-
 ?
L-ornithine the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.47 Sophora flavescens putrescine + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Lupinus angustifolius
? x * 49000, SDS-PAGE Sophora flavescens
? x * 49000, SDS-PAGE Echinosophora koreensis

Synonyms

Synonyms Comment Organism
L/ODC
-
 Lupinus angustifolius
L/ODC
-
 Sophora flavescens
L/ODC
-
 Echinosophora koreensis
LDC
-
 Lupinus angustifolius
LDC
-
 Sophora flavescens
LDC
-
 Echinosophora koreensis
Lys/Orn decarboxylase
-
 Lupinus angustifolius
Lys/Orn decarboxylase
-
 Sophora flavescens
Lys/Orn decarboxylase
-
 Echinosophora koreensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 L-ornithine mutant M341L, pH 7.5, 37°C Lupinus angustifolius
0.4
-
 L-ornithine mutant F344Y, pH 7.5, 37°C Lupinus angustifolius
0.73
-
 L-ornithine pH 7.5, 37°C Echinosophora koreensis
0.91
-
 L-ornithine wild-type, pH 7.5, 37°C Lupinus angustifolius
1.16
-
 L-ornithine pH 7.5, 37°C Sophora flavescens
1.18
-
 L-lysine wild-type, pH 7.5, 37°C Lupinus angustifolius
1.91
-
 L-lysine pH 7.5, 37°C Echinosophora koreensis
2.3
-
 L-ornithine mutant F344H, pH 7.5, 37°C Lupinus angustifolius
2.32
-
 L-lysine pH 7.5, 37°C Sophora flavescens
3.88
-
 L-lysine mutant M341L, pH 7.5, 37°C Lupinus angustifolius
6.33
-
 L-lysine mutant F344Y, pH 7.5, 37°C Lupinus angustifolius
143
-
 L-lysine mutant F344H, pH 7.5, 37°C Lupinus angustifolius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Lupinus angustifolius
7.5
-
-
 Sophora flavescens
7.5
-
-
 Echinosophora koreensis

General Information

General Information Comment Organism
physiological function transgenic tobacco suspension cells and hairy roots expressing lysine decarboxylase produce enhanced levels of cadaverine-derived alkaloids, and transgenic Arabidopsis plants produce enhanced levels of cadaverine, indicating the involvement of this enzyme in lysine decarboxylation to form cadaverine. Enzyme is involved in the decarboxylation of L-Lys, which is the first step of quinolizidine alkaloid biosynthesis in quinolizidine alkaloid-producing plants Lupinus angustifolius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.454
-
 L-ornithine pH 7.5, 37°C Echinosophora koreensis
0.469
-
 L-lysine pH 7.5, 37°C Echinosophora koreensis
0.755
-
 L-ornithine pH 7.5, 37°C Sophora flavescens
1.108
-
 L-lysine pH 7.5, 37°C Sophora flavescens
253
-
 L-ornithine mutant F344Y, pH 7.5, 37°C Lupinus angustifolius
279
-
 L-ornithine mutant M341L, pH 7.5, 37°C Lupinus angustifolius
433
-
 L-lysine wild-type, pH 7.5, 37°C Lupinus angustifolius
859
-
 L-ornithine wild-type, pH 7.5, 37°C Lupinus angustifolius
1203
-
 L-lysine mutant F344Y, pH 7.5, 37°C Lupinus angustifolius
1471
-
 L-lysine mutant F344H, pH 7.5, 37°C Lupinus angustifolius
1496
-
 L-ornithine mutant F344H, pH 7.5, 37°C Lupinus angustifolius
1558
-
 L-lysine mutant M341L, pH 7.5, 37°C Lupinus angustifolius