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4.1.1.49: phosphoenolpyruvate carboxykinase (ATP)

This is an abbreviated version!
For detailed information about phosphoenolpyruvate carboxykinase (ATP), go to the full flat file.

Word Map on EC 4.1.1.49

Reaction

ATP
+
oxaloacetate
=
ADP
 +
phosphoenolpyruvate
 +
CO2

Synonyms

ADP-linked PEP carboxykinase, ATP, oxaloacetate carboxy-lyase (transphosphorylating), ATP-dependent phosphoenolpyruvate carboxykinase, ATP-dependent phosphoenolpyruvate(PEP)carboxykinase, ATP-forming PEP-carboxykinase, ATP-oxaloacetate carboxylase (transphosphorylating), ATP: oxaloacetate carboxy-lyase, ATP:oxaloacetate carboxy-lyase, ATP:oxaloacetate carboxylyase (transphosphorylating), Carboxykinase, phosphopyruvate (adenosine triphosphate), Glycosomal protein P60, LMJF_27_1810, OsPPCK1, OsPPCK3, PCK, pckA, PEP carboxykinase, PEP carboxylase, PEP carboxylase kinase, PEPC, PEPC kinase, PEPCK, PEPCK (ATP), PEPCK-ATP, PEPK, PfPEPCK, phosphoenolpyruvate carboxykinase, phosphoenolpyruvate carboxykinase ATP-dependent, phosphoenolpyruvate carboxykinase [ATP], Phosphoenolpyruvate carboxylase, Phosphoenolpyruvate carboxylase (ATP), phosphoenolpyruvate carboxylase kinase, phosphoenolpyruvate(PEP)carboxykinase (GTP/ATP:oxaloacetate carboxy-lyase (transphosphorylating)EC 4.1.1.32/49), phosphoenolpyruvic carboxykinase, Phosphoenolpyruvic carboxylase, phosphopyruvate carboxykinase, Phosphopyruvate carboxykinase (adenosine triphosphate), PPC, PPCK, Protein p60, yeast PEP carboxykinase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.49 phosphoenolpyruvate carboxykinase (ATP)

Cloned

Cloned on EC 4.1.1.49 - phosphoenolpyruvate carboxykinase (ATP)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA clone
-
cloned in Escherichia coli and in in the PEP carboxykinase-deficient Saccharomyces cerevisiae-strain PUK-3B
-
expressed in Escherichia coli
expressed in Escherichia coli BA203 cells
-
expressed in Escherichia coli ER2566 cells
expressed in pyruvate carboxylase-negative Saccharomyces cerevisiae strain IMK157, IMW001, and IMW002
-
expressed in the PEP carboxykinase-deficient Saccharomyces cerevisiae strain PUK-3B
-
expression in Escherichia coli K-12. PEPCK overexpression has no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant Escherichia coli strain K-12 ppc::kan, PEPCL overexpression increases succinate production 6.5fold
-
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
increasing the acetyl-CoA pool in the presence of overexpressed phosphoenolpyruvate carboxylase enhances succinate production in Escherichia coli
Sorghum sp.
-
mutant enzyme Y207L is expressed in the PEP carboxykinase-deficient Saccharomyces cerevisiae strain PUK-3B
-
mutant plasmid is transformed into the phosphoenolpyruvate carboxykinase-deficient yeast strain PUK-3B (MATalpha pck ura3)
overexpressed
-
transformed into the PEP carboxykinase-deficient yeast strain PUK-3B (MATalpha pck ura3)