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4.1.1.49: phosphoenolpyruvate carboxykinase (ATP)

This is an abbreviated version!
For detailed information about phosphoenolpyruvate carboxykinase (ATP), go to the full flat file.

Word Map on EC 4.1.1.49

Reaction

ATP
+
oxaloacetate
=
ADP
 +
phosphoenolpyruvate
 +
CO2

Synonyms

ADP-linked PEP carboxykinase, ATP, oxaloacetate carboxy-lyase (transphosphorylating), ATP-dependent phosphoenolpyruvate carboxykinase, ATP-dependent phosphoenolpyruvate(PEP)carboxykinase, ATP-forming PEP-carboxykinase, ATP-oxaloacetate carboxylase (transphosphorylating), ATP: oxaloacetate carboxy-lyase, ATP:oxaloacetate carboxy-lyase, ATP:oxaloacetate carboxylyase (transphosphorylating), Carboxykinase, phosphopyruvate (adenosine triphosphate), Glycosomal protein P60, LMJF_27_1810, OsPPCK1, OsPPCK3, PCK, pckA, PEP carboxykinase, PEP carboxylase, PEP carboxylase kinase, PEPC, PEPC kinase, PEPCK, PEPCK (ATP), PEPCK-ATP, PEPK, PfPEPCK, phosphoenolpyruvate carboxykinase, phosphoenolpyruvate carboxykinase ATP-dependent, phosphoenolpyruvate carboxykinase [ATP], Phosphoenolpyruvate carboxylase, Phosphoenolpyruvate carboxylase (ATP), phosphoenolpyruvate carboxylase kinase, phosphoenolpyruvate(PEP)carboxykinase (GTP/ATP:oxaloacetate carboxy-lyase (transphosphorylating)EC 4.1.1.32/49), phosphoenolpyruvic carboxykinase, Phosphoenolpyruvic carboxylase, phosphopyruvate carboxykinase, Phosphopyruvate carboxykinase (adenosine triphosphate), PPC, PPCK, Protein p60, yeast PEP carboxykinase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.49 phosphoenolpyruvate carboxykinase (ATP)

Crystallization

Crystallization on EC 4.1.1.49 - phosphoenolpyruvate carboxykinase (ATP)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1.85 A and 1.7 A resolution structures of the native and pyruvate/Mn2+/phosphate complex, space group of native enzyme is P4(3), space group for enzyme complex is P2(1), unit-cell parameters for native enzyme: a = b = 102.09 A, c = 72.12 A, beta = 90°. Unit-cell parameters for the enzyme complex: a = 56.74 A, b = 55.09 A, c = 90.08 A, beta = 106.17°
hanging drop vapor diffusion method. 2.2 A resolution crystal structure of the enzyme complexed with ATP, Mg2+, Mn2+ and the transition state analogue oxalate. 2.4 A resolution structure of the native enzyme. Unit cell dimensions of the enzyme complex: a = 194.87 A, b = 123.22 A, c = 48.46 A, alpha = beta = gamma = 90°C. Unit cell dimensions of the native enzyme: a = 129.66 A, b = 55.73 A, c = 139.4 A, alpha = 90°, beta = 93.77°, gamma = 90°
crystals grown by hanging drop vapor diffusion, ADP-ALF3-Mg2+ complex, monoclinic space group C2 with unit cell parameters a : 126.0 A, b : 95.8 A, c : 46.6 A, ADP-ALF3-Mg2+-pyruvate complex, monoclinic space group C2 with unit cell parameters a : 124.7 A, b : 94.2 A, c : 46.3 A
-
crystals grown by hanging drop vapor diffusion, monoclinic space group C2, unit cell parameter a = 126.2 A, b = 95.2 A, c = 46.8 A, d = 95.2 A
-
space group P2(1)2(1)2(1), a : 77.7 A, b : 89.7 A, c 93.8 A
-
three-dimensional crystal structure, enzyme possesses a mononucleotide-binding domain unique from other proteins having the P-loop motif
molecular modeling of structure
oil-microbatch method, the unliganded form crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.7, b = 129.91 A, c = 173.39 A. The ATP-liganded crystals are nearly isomorphous to the unliganded crystals. They belong to space group P2(1)2(1)2(1), with unit cell parameters a = 64.07 A, b = 130.55 A, c = 174.2 A
sitting drop vapour diffusion, primitive orthorhombic crystals, space group P2(1)2(1)2(1), a : 65.97 A, b : 107.61 A, c : 179.08 A