4.1.1.49: phosphoenolpyruvate carboxykinase (ATP)
This is an abbreviated version!
For detailed information about phosphoenolpyruvate carboxykinase (ATP), go to the full flat file.
Word Map on EC 4.1.1.49
Reaction
Synonyms
ADP-linked PEP carboxykinase, ATP, oxaloacetate carboxy-lyase (transphosphorylating), ATP-dependent phosphoenolpyruvate carboxykinase, ATP-dependent phosphoenolpyruvate(PEP)carboxykinase, ATP-forming PEP-carboxykinase, ATP-oxaloacetate carboxylase (transphosphorylating), ATP: oxaloacetate carboxy-lyase, ATP:oxaloacetate carboxy-lyase, ATP:oxaloacetate carboxylyase (transphosphorylating), Carboxykinase, phosphopyruvate (adenosine triphosphate), Glycosomal protein P60, LMJF_27_1810, OsPPCK1, OsPPCK3, PCK, pckA, PEP carboxykinase, PEP carboxylase, PEP carboxylase kinase, PEPC, PEPC kinase, PEPCK, PEPCK (ATP), PEPCK-ATP, PEPK, PfPEPCK, phosphoenolpyruvate carboxykinase, phosphoenolpyruvate carboxykinase ATP-dependent, phosphoenolpyruvate carboxykinase [ATP], Phosphoenolpyruvate carboxylase, Phosphoenolpyruvate carboxylase (ATP), phosphoenolpyruvate carboxylase kinase, phosphoenolpyruvate(PEP)carboxykinase (GTP/ATP:oxaloacetate carboxy-lyase (transphosphorylating)EC 4.1.1.32/49), phosphoenolpyruvic carboxykinase, Phosphoenolpyruvic carboxylase, phosphopyruvate carboxykinase, Phosphopyruvate carboxykinase (adenosine triphosphate), PPC, PPCK, Protein p60, yeast PEP carboxykinase
ECTree
4.1.1.49 phosphoenolpyruvate carboxykinase (ATP)Advanced search results
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results (KM Value
KM Value on EC 4.1.1.49 - phosphoenolpyruvate carboxykinase (ATP)
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
additional information
additional information
MnATP2- as substrate, for S252A mutant Km above 3000 microM
-
0.01
ADP
-
mutant enzyme E299A, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.013
ADP
-
mutant enzyme Y207L, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.016
ADP
-
wild type enzyme, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.034
ADP
-
wild type enzyme, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.077
ADP
for the pyruvate kinase-like activity of S252A mutant
0.099
ADP
-
mutant enzyme L460A, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.17
ADP
-
F216Y: kinetic parameter in the PEP carboxylation direction
0.026
ATP
-
mutant enzyme E299A, in 100 mM MOPS buffer (pH 7.0), at 30°C
0.03
ATP
-
wild type enzyme, in 100 mM MOPS buffer (pH 7.0), at 30°C
0.204
ATP
-
mutant enzyme L460A, in 100 mM MOPS buffer (pH 7.0), at 30°C
0.024
CO2
-
F216Y: kinetic parameter in the PEP carboxylation direction
0.36
CO2
-
condition: 0.1 mM Mn2+, 5 mM Mg2 /Vmax: 16 micromol/min/mg
1.9
CO2
-
condition: 0.1 mM Mn2+, 5 mM Mg2 /Vmax: 37 micromol/min/mg
2 - 3
CO2
-
mutant enzyme K212M, in 100 mM MOPS buffer (pH 6.6), at 30°C
8.4
CO2
-
condition: 4 mM Mn2+ /Vmax: 47 micromol/min/mg
9
CO2
-
mutant enzyme E272Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
11
CO2
-
mutant enzyme K213Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
14
CO2
-
mutant enzyme E284Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
14
CO2
-
mutant enzyme L460A, in 100 mM MOPS buffer (pH 6.6), at 30°C
29
CO2
-
mutant enzyme Y207L, in 100 mM MOPS buffer (pH 6.6), at 30°C
47
CO2
-
mutant enzyme E299A, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.012
Mn2+
-
pH high (pH range:6-8.4), pH:12, PEP carboxylation direction
0.056
Mn2+
-
F216Y: kinetic parameter in the PEP carboxylation direction
0.345
Mn2+
-
F216Y: kinetic parameter in the PEP formation direction
0.066
oxalacetate
-
kinetic parameter in the PEP formation direction
0.122
oxalacetate
for the oxaloacetate decarboxylase activity of wild-type
1.079
oxalacetate
-
F216Y: kinetic parameter in the PEP formation direction
5.2
oxalacetate
for the oxaloacetate decarboxylase activity of S252A mutant
0.014
oxaloacetate
-
condition: + 2 mM ADP /Vmax: 0.32 micromol/min/mg
0.024
oxaloacetate
-
mutant enzyme K213Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.051
oxaloacetate
-
mutant enzyme L460A, in 100 mM MOPS buffer (pH 7.0), at 30°C
0.066
oxaloacetate
-
wild type enzyme, in 100 mM MOPS buffer (pH 7.0), at 30°C
0.068
oxaloacetate
-
mutant enzyme K212M, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.079
oxaloacetate
-
condition: no ADP /Vmax: 3.27 micromol/min/mg
0.13
oxaloacetate
-
wild type enzyme, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.13
oxaloacetate
-
mutant enzyme E299A, in 100 mM MOPS buffer (pH 7.0), at 30°C
0.2
oxaloacetate
-
mutant enzyme K212R, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.238
oxaloacetate
-
mutant enzyme E272Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.99
oxaloacetate
-
condition: + 2 mM ADP /Vmax: 3.36 micromol/min/mg
2
oxaloacetate
-
Km above 2.0 mM, mutant enzyme E284Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.037
phosphoenolpyruvate
for the pyruvate kinase-like activity of S252A mutant
0.085
phosphoenolpyruvate
-
pH 6.5, 30°C, mutant enzyme H225E
0.104
phosphoenolpyruvate
-
mutant enzyme L460A, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.108
phosphoenolpyruvate
-
mutant enzyme E299A, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.135
phosphoenolpyruvate
for the pyruvate kinase-like activity of wild-type
0.17
phosphoenolpyruvate
-
mutant enzyme E284Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.185
phosphoenolpyruvate
-
mutant enzyme E272Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.216
phosphoenolpyruvate
-
mutant enzyme K212M, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.219
phosphoenolpyruvate
-
pH 6.5, 30°C, wild-type enzyme
0.289
phosphoenolpyruvate
-
pH 6.5, 30°C, mutant enzyme D263E
0.307
phosphoenolpyruvate
-
kinetic parameter in the PEP carboxylation direction
0.307
phosphoenolpyruvate
-
wild type enzyme, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.32
phosphoenolpyruvate
-
mutant enzyme K213Q, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.5
phosphoenolpyruvate
-
mutant enzyme Y207L, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.651
phosphoenolpyruvate
-
mutant enzyme K212R, in 100 mM MOPS buffer (pH 6.6), at 30°C
0.917
phosphoenolpyruvate
-
F216Y: kinetic parameter in the PEP carboxylation direction
3
phosphoenolpyruvate
-
wild type enzyme, in 100 mM MOPS buffer (pH 6.6), at 30°C
