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Literature summary for 4.1.1.49 extracted from
- Molecular and biochemical characterization of phosphoenolpyruvate carboxykinase in the ruminal bacterium Ruminococcus albus (2009), Curr. Microbiol., 58, 416-420.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Ruminococcus albus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
phosphoenolpyruvate | in 50 mM HEPES (pH 7.0) | Ruminococcus albus |  |
| 0.81 | - |
ADP | in 50 mM HEPES (pH 7.0) | Ruminococcus albus | |
| 1.81 | - |
oxaloacetate | in 50 mM HEPES (pH 7.0) | Ruminococcus albus | |
| 1.83 | - |
ATP | in 50 mM HEPES (pH 7.0) | Ruminococcus albus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 100% activity in the presence of 10 mM Mg2+ | Ruminococcus albus | |
| Mn2+ | 85.3% activity in the presence of 10 mM Mn2+ | Ruminococcus albus | |
| additional information | not activated by Co2+, Ni2+, Cu2+, or Zn2+ | Ruminococcus albus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59430 | - |
calculated from amino acid sequence | Ruminococcus albus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminococcus albus | B3Y6D3 | strain ATCC27210[T] | - |
| Ruminococcus albus ATCC 27210[T] | B3Y6D3 | strain ATCC27210[T] | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap chelating affinity column chromatography and Superdex 200 gel filtration | Ruminococcus albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + oxaloacetate | - |
Ruminococcus albus | ADP + phosphoenolpyruvate + CO2 | ADP is the preferred PCK phosphate acceptor | r | |
| ATP + oxaloacetate | - |
Ruminococcus albus ATCC 27210[T] | ADP + phosphoenolpyruvate + CO2 | ADP is the preferred PCK phosphate acceptor | r | |
| GTP + oxaloacetate | when ADP is replaced by GDP in the PEP-carboxylating reaction, PCK activity decreases approximately 5fold | Ruminococcus albus | GDP + phosphoenolpyruvate + CO2 | - |
r | |
| GTP + oxaloacetate | when ADP is replaced by GDP in the PEP-carboxylating reaction, PCK activity decreases approximately 5fold | Ruminococcus albus ATCC 27210[T] | GDP + phosphoenolpyruvate + CO2 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCK | - |
Ruminococcus albus |
| phosphoenolpyruvate carboxykinase | - |
Ruminococcus albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Ruminococcus albus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Ruminococcus albus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | preferred cofactor | Ruminococcus albus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ruminococcus albus | calculated from amino acid sequence | - |
4.9 |
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Release 2023.1 (January 2023)
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