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4.1.1.25: tyrosine decarboxylase

This is an abbreviated version!
For detailed information about tyrosine decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.25

Reaction

L-tyrosine
=
tyramine
 +
CO2

Synonyms

AADC, AtTYDC, Bradi2g51170, Decarboxylase, tyrosine, dTdc1, dTdc2, ELI5, L-(-)-Tyrosine apodecarboxylase, L-amino acid decarboxylase, L-Tyrosine decarboxylase, LbTDC, LOC100840315, MfnA protein, P0665A11.14, PcTYDC2, PF1159, PsTYDC1, PsTYDC2, TDC, tdcA, TYDC, TYDC/DODC, TYDC1, Tydc9, TYR decarboxylase, TyrDC, TyrDC-2, tyrosine decarboxylase, tyrosine decarboxylase-2, Tyrosine/Dopa decarboxylase, tyrosine/Dopa decarboxylase-1, tyrosine/Dopa decarboxylase-2, VwTYDC

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.25 tyrosine decarboxylase

Crystallization

Crystallization on EC 4.1.1.25 - tyrosine decarboxylase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified His-tagged enzyme as apoenzyme and in complex with pyridoxal 5'-phosphate (PLP), sitting drop vapor diffusion method, mixing of 0.002 ml of 10 mg/ml protein solution with 0.002 ml of reservoir solution containing 0.1 M sodium cacodylate trihydrate, pH 7.5, 0.2 M MgCl2, and 18% PEG 1000, for the SeMet-LbTDC enzyme variant 0.1 M sodium cacodylate trihydrate, pH 7.0, 0.1 M MgCl2, and 16% PEG 4000 is used, the LbTDC-PLP complex is also crystallized at a molar ratio of 1:4 under the same conditions as SeMet-LbTDC, 2-5 days, 18°C, X-ray diffraction structure determination and analysis at 1.73-1.90 A resolution
-
purified recombinant tagged enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 25 mM HEPES, pH 7.0, and 0.04 mM pyridoxal 5'-phosphate, with 0.001 ml of reservoir solution containing 0.2 M NaOAc and 1 M NH4H2PO4, and equilibration against 0.45 ml of reservoir solution, X-ray diffraction structure determination and analysis at 3.1 A resolution
-