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3.6.4.B7: RadA recombinase

This is an abbreviated version!
For detailed information about RadA recombinase, go to the full flat file.

Word Map on EC 3.6.4.B7

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

DNA repair and recombination protein, DNA repair protein RAD51 homolog 1, Hvo RadA, MvRadA, Pho RadA, PhoRadA, Rad51, RadA, RadA intein, RadA recombinase, RadA/Sms, RadC1, RadC2, SMS, SSO0250, SsoRadA, SsoRadA recombinase, SsRada

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.B7 RadA recombinase

Temperature Stability

Temperature Stability on EC 3.6.4.B7 - RadA recombinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
after boiling of 0.18 mM enzyme in 20 mM NaCl for 30 min, the enzyme retains one-third of its original activity. Two factors, preliminary presynaptic complex formation and high protein concentration, save, at least partially, the activity of RadADa protein, even at 100°C
25 - 87
the enzyme maintains its secondary structure and activities in vitro at high temperatures, up to 87°C. It also shows high stability of 18.3 kcal/mol at 25°C and neutral pH
81
recombinant mutant HumRadA2 (I169M/Y201A/V202Y/E219S/D220A/K221M)
90
prerincubation of 0.015 mM enzyme for 6 min, complete inactivation of the protein
94
purified recombinant His-tagged RadA, remains almost unaffected after 4 h incubation
95
full-lengt and truncated RadA-ct prove to be extremely thermostable and fail to denature at a temperature of 95°C
additional information