Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | addition of 100 mM NaCl results in a 3.2-fold decrease of the monomer kcat value | Desulfurococcus amylolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 20 mM NaCl used in this mixture was found to be optimal for ATP hydrolysis | Desulfurococcus amylolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfurococcus amylolyticus | Q9Y8J4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | at optimal assay conditions, the RadADa presynaptic complex is able to hydrolyze ATP efficiently with ssDNA cofactors of 940 nt and greater. Circular and linearized M13 ssDNA demonstrate the same ability to stimulate ATP hydrolysis as a linearized dsDNA of this phage, whereas the supercoiled dsDNA (replicative form I) is a weak cofactor due to the only partial denaturation at 90°C | Desulfurococcus amylolyticus | ADP + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
assay at | Desulfurococcus amylolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 102 | preformation of presynaptic complex at 80°C guarantees the conservation of at least a part of the activity up to 102°C. Activity is close to zero at temperatures below 65°C | Desulfurococcus amylolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
prerincubation of 0.015 mM enzyme for 6 min, complete inactivation of the protein | Desulfurococcus amylolyticus |
100 | - |
after boiling of 0.18 mM enzyme in 20 mM NaCl for 30 min, the enzyme retains one-third of its original activity. Two factors, preliminary presynaptic complex formation and high protein concentration, save, at least partially, the activity of RadADa protein, even at 100°C | Desulfurococcus amylolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
ATP | pH 7.5, 90°C | Desulfurococcus amylolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Desulfurococcus amylolyticus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme promotes recombination at temperatures approaching the DNA melting point | Desulfurococcus amylolyticus |