Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.B7 extracted from

  • Stefanska, A.; Gaffke, L.; Kaczorowska, A.K.; Plotka, M.; Dabrowski, S.; Kaczorowski, T.
    Highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances specificity of simplex and multiplex PCR assays (2016), J. Appl. Genet., 57, 239-249.
    View publication on PubMed
Search for more literature for 3.6.4.B7

Application

Application Comment Organism
analysis the highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances the specificity of simplex and multiplex PCR assays Pyrococcus woesei
diagnostics the RadA protein proves to be useful in allelic discrimination assay of HADHA gene associated with long-chain 3-hydroxylacyl-CoA dehydrogenase deficiency that in infancy may lead to hypotonia, serious heart and liver problems and even sudden death Pyrococcus woesei

Cloned(Commentary)

Cloned (Comment) Organism
gene radA DNA and amino acid sequence determination and analysis, recombinant overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pyrococcus woesei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pyrococcus woesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Pyrococcus woesei
-
 ADP + phosphate
-
 ?
ATP + H2O Pyrococcus woesei DSM 3773
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus woesei A0A0D3MA54 gene radA
-
Pyrococcus woesei DSM 3773 A0A0D3MA54 gene radA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) using ammonium sulfate fractionation and nickel affinity chromatography, to homogeneity Pyrococcus woesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Pyrococcus woesei ADP + phosphate
-
 ?
ATP + H2O
-
 Pyrococcus woesei DSM 3773 ADP + phosphate
-
 ?
additional information enzyme RadA binds both to ssDNA and dsDNA. The highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances the specificity of simplex and multiplex PCR assays Pyrococcus woesei ?
-
 ?
additional information enzyme RadA binds both to ssDNA and dsDNA. The highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances the specificity of simplex and multiplex PCR assays Pyrococcus woesei DSM 3773 ?
-
 ?

Subunits

Subunits Comment Organism
undecamer
-
 Pyrococcus woesei

Synonyms

Synonyms Comment Organism
RadA
-
 Pyrococcus woesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Pyrococcus woesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
94
-
 purified recombinant His-tagged RadA, remains almost unaffected after 4 h incubation Pyrococcus woesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 ATPase assay at Pyrococcus woesei
8
-
 DNA binding assay at Pyrococcus woesei