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3.6.4.B7: RadA recombinase

This is an abbreviated version!
For detailed information about RadA recombinase, go to the full flat file.

Word Map on EC 3.6.4.B7

Reaction

ATP
+
H2O
=
ADP
+
phosphate

Synonyms

DNA repair and recombination protein, DNA repair protein RAD51 homolog 1, Hvo RadA, MvRadA, Pho RadA, PhoRadA, Rad51, RadA, RadA intein, RadA recombinase, RadA/Sms, RadC1, RadC2, SMS, SSO0250, SsoRadA, SsoRadA recombinase, SsRada

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.B7 RadA recombinase

Cloned

Cloned on EC 3.6.4.B7 - RadA recombinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene radA DNA and amino acid sequence determination and analysis, recombinant overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene radA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and genotyping of deep sea isolates using the enzyme sequence, overview
-
gene radA, highly efficient cis-splicing of PhoRadA intein, recombinant expression of His-tagged enzyme in Escherichia coli strain ER2566
gene radA, recombinant coexpression of His-tagged RadA with untagged RadB (UniProt ID D4GZB4), which form the RadAB complex, in Escherichia coli strain Rosetta2 (DE3) with deleted gene arnA. The ratio of RadA to RadB is 4:1, which suggests that the proteins interact with a specific stoichiometry
gene radA, recombinant enzyme expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)[pLysS] from plasmid pCB1020 under the control of a rifampicin-resistant promoter, PT7
gene radA, recombinant expression in Escherichia coli strain BL21 Star (DE3), construction of the plasmid pMIG-RadAi, carrying the Pho RadA intein gene in foreign exteins, is accomplished by inserting the RadA intein sequence with short native exteins into ClaI and SphI sites between sequences coding for MBP and GFP in a pACYCDuet-1 plasmid backbone, splicing of the RadA intein located in the ATPase domain of the hyperthermophilic archaeon Pyrococcus horikoshii is strongly regulated by the native exteins, which lock the intein in an inactive state
gene radA, recombinant expression in Escherichia coli strain Rosetta
gene radA, recombinant overexpression in Escherichia coli strain Rosetta
hexagonal MmRadA crystals (space group P61) are grown by the hanging-drop vapour-diffusion method and grew to maximum dimensions of 0.1 x 0.1 x 0.3 mm. Crystal structure of enzyme in complex with AMPPNP, crystal structure of the enzyme in complex with AMPPNP and potassium ions and crystal structure of the enzyme in complex with AMPPNP and ammonium ions
overexpressed in Escherichia coli
recombinant expression of codon-optimized MBP-tagged and Strep-II-tagged wild-type and mutant RadAs proteins in Escherichia coli strain BL21(DE3)
recombinant expression of the RadAC-terminal domain RadA-ct in Escherichia coli as soluble protein
recombinant overexpression in S33 cells, RAD51 fibers appear to be excluded from nucleoli, overexpression of RAD51 can cause accumulation of RAD51 foci at sites that do not contain ss-DNA