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3.6.4.B7: RadA recombinase

This is an abbreviated version!
For detailed information about RadA recombinase, go to the full flat file.

Word Map on EC 3.6.4.B7

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

DNA repair and recombination protein, DNA repair protein RAD51 homolog 1, Hvo RadA, MvRadA, Pho RadA, PhoRadA, Rad51, RadA, RadA intein, RadA recombinase, RadA/Sms, RadC1, RadC2, SMS, SSO0250, SsoRadA, SsoRadA recombinase, SsRada

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.B7 RadA recombinase

Metals Ions

Metals Ions on EC 3.6.4.B7 - RadA recombinase

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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
Mg2+ als well as K+ ions are absorbed at the ATPase center. K+ (but not Na+), stimulates the ATP hydrolysis reaction with an apparent dissociation constant of about 40 mM. The strand exchange activity of the wild-type enzyme is also stimulated by potassium with an apparent dissociation constant of 35 mM
KCl
stimulates the ATPase activity in the absence of ssDNA as well as the strand-exchange activity in the presence of AMPPNP
Mn2+
the enzyme requires the presence of bivalent cations, such as Mg2+ and Mn2+
NaCl
20 mM NaCl used in this mixture was found to be optimal for ATP hydrolysis
NH4Cl
stimulates the ATPase activity in the absence of ssDNA as well as the strand-exchange activity in the presence of AMPPNP
RbCl
stimulates the ssDNA-dependent ATPase activity. 1.0 M RbCl does not stimulate the ATPase activity of MmRadA in the absence of DNA