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3.6.4.13: RNA helicase

This is an abbreviated version!
For detailed information about RNA helicase, go to the full flat file.

Word Map on EC 3.6.4.13

Reaction

a nucleoside triphosphate
+
H2O
=
a nucleoside diphosphate
 +
phosphate

Synonyms

1a NTPase/helicase, Aquarius, AtHELPS, ATP-dependent helicase, ATP-dependent RNA helicase, ATP-dependent RNA helicase DDX3X, ATP-dependent RNA helicase DDX5, ATP/dATP-dependent RNA helicase, ATPase, ATPase/helicase, ATPase/RNA helicase, AtRH3, AtRH7, bel, BmL3-helicase, BMV 1a protein, BRR2, Brr2 RNA helicase, Brr2p, Cbu_0670, ChlR1 helicase, cold-shock DEAD-box protein A, CrhR, CsdA, CshA, CTHT_0005780, CTHT_0009470, DBP2, DbpA, DDX17, DDX19, DDX19B, DDX21, DDX21 RNA helicase, DDX25, DDX27, DDX3, DDX3X, DDX3Y, DDX4, DDX5, DDX58, DeaD, DEAD box helicase, DEAD box RNA helicase, DEAD-box ATP-dependent RNA helicase CshA, DEAD-box helicase, DEAD-box protein DED1, DEAD-box RNA helicase, DEAD-box rRNA helicase, DEAH box protein 34, DEAH-box protein 2, DEAH-box RNA helicase, DEAH/RHA RNA helicase, DED1, DENV NS3H, DEx(H/D)RNA helicase, DExD/H box RNA helicase, DEXD/H-box RNA helicase, dexh helicase, DExH protein RNA helicase A, DExH/D-Box protein RNA helicase A, DExH/D-box RNA helicase, DHX34, DHX36, DHX8, DHX9, Dhx9/RNA helicase A, DNA/RNA-dependent ATPase, EhDEAD1, EhDEAD1 RNA helicase, eIF4A, eIF4A helicase, eIF4AIII, eukaryotic initiation factor eIF 4A, frequency-interacting RNA helicase, FRH, FRQ-interacting RNA helicase, gonadotropin-regulated testicular RNA helicase, GRTH, GRTH/DDX25, HCV NS3 helicase, HEL-1, helicase, helicase 1, helicase B, helicase/nucleoside triphosphatase, HRpA, IBP160, increased size exclusion limit2, intron-binding protein 160, ISE2, KOKV helicase, Lmo1722, mitochondrial DEAD-box RNA helicase, Mss116p, mtr4, Mtr4p, NA-helicase, non structural protein 3, non-structural 3, non-structural protein 3, non-structural protein 3 protein, nonstructural protein 3, NPH-II, NS3, NS3 ATPase/helicase, NS3 helicase, NS3 NTPase/helicase, NS3 protein, NTPase/helicase, nucleoside 5'-triphosphatase, nucleoside triphosphatase/helicase, nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase, NWMN_1985, p54 RNA helicase, p68, p68 RNA helicase, P72, PH1280, pre-mRNA-splicing ATP-dependent RNA helicase 28, protein NS3, Prp28, Prp43, Prp5, RENT1, RH22, RHA, RhlB, RIG-I, Rm62, RNA DEAD-box helicase, RNA helicase, RNA helicase A, RNA helicase Aquarius, RNA helicase BELLE, RNA helicase CrhR, RNA helicase CsdA, RNA helicase CshA, RNA helicase DDX17, RNA helicase DDX27, RNA helicase DDX3, RNA helicase Ddx39, RNA helicase DDX3X, RNA helicase DDX5, RNA helicase DDX6, RNA helicase DeaD, RNA helicase DHX34, RNA helicase DHX8, RNA helicase DHX9, RNA helicase HEL-1, RNA helicase Hera, RNA helicase ISE2, RNA Helicase p68, RNA helicase RHAU, RNA helicase UPF1, RNA splicing effector, RNA-dependent ATPase, RNA-dependent NTPase/helicase, RNA-helicase, RTPase, SA1885, Ski2-like helicase, slr0083, SNRNP200, spliceosomal DEAH-box RNA helicase, spliceosomal RNA helicase, SpolvlgA, Supv3L1, TaRH1, TGBp1 NTPase/helicase domain, Tk-DeaD, Triticum aestivum RNA helicase, Upf1, UPF1_1, UPF1_2, Vasa, VRH1, YxiN, ZmRH3, ZmRH3A, ZmRH3B

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.13 RNA helicase

Cloned

Cloned on EC 3.6.4.13 - RNA helicase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovirus expression system
C-terminal domain of Hera is overproduced in Escherichia coli
-
construction of a di-cistronic vector that overexpresses a complex comprising RhlB and its recognition site within RNase E, corresponding to residues 696-762, the expression construct is termed pRneRhlBDELTA1-397. Expression of His-tagged wild-type and mutant RhlB and RNaseE in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli
-
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL-21
-
expressed in Escherichia coli BL21 (DE3), recombinant protein, pET21b vector
expressed in Escherichia coli BL21(DE3), recombinant protein, NS3d2wt variant corresponding to wild-type domain 2, NS3d2D construct comprises the complete domain, HCV(1361-1503) without loop, pET21b andpET16b vectors
expressed in Escherichia coli Rosetta 2 (DE3) cells
Thermochaetoides thermophila
expressed in Escherichia coli, C-terminal portion with the ATPase/helicase domain, plasmid pET-30a
-
expressed in Escherichia coli, recombinant protein
-
expressed in Escherichia coli, strain Rosetta (DE3), recombinant nonstructural protein 3
-
expressed in Escherichia coli, strains XL-1 Blue, Rosetta (DE3), M15 (pREP4), vector pET-21-2c, kinetics of NS3 protein accumulation upon its expression in Escherichia coli at 25°C for 1-5 h shown
-
expression in Escherichia coli
expression of C-terminally His-tagged truncated NS3 NTPase/helicase domain in Escherichia coli
-
expression of GST-fusion wild-type and mutant enzymes in Escherichia coli strain C41(DE3)
-
expression of NS3 as maltose-binding protein furion protein using the constitutive elongation factor-1alpha promoter in HEK-293T cells. Plant viral RSS protein NS3 complements HIV-1 Tat based on the sequestration of small dsRNA
expression of NS3 ATPase/helicase in Escherichia coli, expression of the His-tag C-terminal domain
-
expression of the Arg-rich amino acid motif HCV1487-1500, of the complete domain 2, and of domain 2 lacking the flexible loop localized between Val1458 and Thr1476 as His10-tagged proteins in Escherichia coli strain BL21(DE3)
expression of the C-terminal helicase domain (amino-acid sequence corresponding to that between residues 189 and 620 of the predicted NS3 polypeptide) in Escherichia coli
-
expression of the His-tagged catalytic domain of the NS3 helicase domain from dengue virus serotype 4 in Escherichia coli strain BL21
-
expression of the N-terminally His-tagged enzyme as soluble protein in Escherichia coli strain BL21(DE3)
EF409381
expression of the soluble His6-tagged C-terminal portion of NS3 in Escherichia coli C41 cells
-
expression of wild-type and mutant His-tagged NS3 helicase domain in Escherichia coli
expression of wild-type and mutant NS3, cloning of a His6-tag to the N-terminus of NS3 greatly increases its affinity for RNA
expression of wild-type and N-terminally truncated enzyme in Escherichia coli
-
gene bel, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme BELLE in fly brain
gene BRR2, recombinant expression of His10-tagged TEV-cleavable codon-optimized DNA fragments encoding selected regions of yBrr2 in Escherichia coli strain Rosetta2 (DE3)
gene cshA, recombinant expression of FLAG-Strep-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta (DE3)
gene cshA, recombinant expression of His-tagged enzyme fragment 1-211, comprising the N-terminal RecA-like domain 1, in Escherichia coli strain Rosetta2 (DE3)
gene cshA, recombinant expression of N-terminally His-tagged wild-type and truncated mutant enzymes in Escherichia coli strain BL21(pLysS), subcloning in Escherichia coli strain DH5alpha
gene CTHT_0009470, recombinant expression of His10-tagged TEV-cleavable codon-optimized DNA fragments encoding selected regions of cBrr2 in Escherichia coli strain Rosetta2 (DE3)
Thermochaetoides thermophila
gene DDX5, is located in the region 17q23-q25
gene ISE2, quantitative RT-PCR expression analysis
gene lmo1722, recombinant expression of His-tagged enzyme in Escherichia coli using a constitutively active promoter
gene PA2840, DNA and amino acid sequence dtermiantion and analysis, recombinant expression of wild-type and mutant enzymes in Eescherichia coli strain Tuner (DE3), quantitative reverse transcriptase PCR
gene RH7, phylogenetic analysis, promoter:GUS transgenic plants AtRH7 quantitative real-time PCR expression analysis. AtRH7 is fused to the C-terminus of YFP and expressed in opnion cells revealing that AtRH7 localizes mainly to the nucleolus and to a minor degree to the nucleoplasm. The enzyme can complement the Escherichia coli DELTAcsdA mutant, which is deficient in growth at low temperatures
gene SNRNP200
gene SNRNP200, recombinant expression of His10-tagged TEV-cleavable codon-optimized DNA fragments encoding selected regions of hBrr2 in Escherichia coli strain Rosetta2 (DE3)
gene TaRH1, DNA and amino acid sequence determination and analysis, phylogenetic tree and analysis, quantitative real-time PCR enzyme expression analysis, the gene encoding the enzyme is differentially expressed under both biotic and several abiotic stresses, TaRH1-catalysed unwinding of duplex RNA following recombinant His-tagged TaRH1 expression in Escherichia coli strain BL21(DE3)
NS3-plus and NS3/4a-plus genes expressed in Escherichia coli, composition of NS3-4A expression product using the pet-SUMO vector
NS3-plus and NS3/4a-plus genes expressed in Escherichia coli, generation of NS3-4A expression product, pET15b and pet-SUMO vector
-
overexpressed as His-tag fusion protein in Escherichia coli
EF409381
overexpression in Escherichia coli
overexpression of the DEADbox mitif II mutant enzyme in N2a cells leading to reduced Let-7a activity
overproduction of full-lenght PH1280p in Escherichia coli cells
phylogenetic analysis
recombinant expression of C-terminally His-tagged and N-terminally calmodulin-binding-protein-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Rosetta
recombinant expression of C-terminally His6-GST-tagged full-length DHX8 (fl-DHX8) and truncated construct containing the complete catalytically active C-terminal helicase core region (DHX8DELTA547) in Spodoptera frugiperda Sf9 cells using the baculovirus expression system
recombinant expression of FLAG-tagged DHX34 in HEK-293T cells, co-expression with recombinant UPF1 protein, DHX34 and UPF1 interact directly, but weak interaction with UPF2 and UPF3b
recombinant expression of FLAG3-tagged wild-type and mutant enzymes in HEK293T cells
recombinant expression of His-tagged enzyme
recombinant expression of N-terminally His-tagged wild-type and engineered enzymes in Escherichia coli
recombinant expression of N-terminally His6-tagged enzyme in Spodooptera frugiperda Sf9 cells
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in HEK293e cells
recombinant expression of wild-type and mutant isozymes, that are CBP-tagged at the N-terminus and His-tagged at the C-terminus, in Escherichia coli strain BL21(DE3)
recombinant expression of wild-type and truncated mutant enzymes in Escherichia coli
recombinant His-tagged enzyme expression in Spodoptera frugiperda Sf9 cells using the baculovirus expression system
recombinantly expressed as a His-tagged fusion protein
X-chromosome-encoded RNA helicase DDX3X