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Literature summary for 3.6.4.13 extracted from

  • Schnettler, E.; de Vries, W.; Hemmes, H.; Haasnoot, J.; Kormelink, R.; Goldbach, R.; Berkhout, B.
    The NS3 protein of rice hoja blanca virus complements the RNAi suppressor function of HIV-1 Tat (2009), EMBO Rep., 10, 258-263.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.13

Cloned(Commentary)

Cloned (Comment) Organism
expression of NS3 as maltose-binding protein furion protein using the constitutive elongation factor-1alpha promoter in HEK-293T cells. Plant viral RSS protein NS3 complements HIV-1 Tat based on the sequestration of small dsRNA Rice hoja blanca tenuivirus

Protein Variants

Protein Variants Comment Organism
additional information an NS3 mutant, that is deficient in RNA binding and its associated RSS activity, is inactive in complementing the RNA silencing suppressor function of the Tat protein of Human immunodeficiency virus type 1 Rice hoja blanca tenuivirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, recombinant NS3, SDS-PAGE, x * 109000, recombinant MBP-fusion NS3 protein, SDS-PAGE Rice hoja blanca tenuivirus
109000
-
 x * 66000, recombinant NS3, SDS-PAGE, x * 109000, recombinant MBP-fusion NS3 protein, SDS-PAGE Rice hoja blanca tenuivirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rice hoja blanca tenuivirus the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells ?
-
 ?
additional information Rice hoja blanca tenuivirus RHBV the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rice hoja blanca tenuivirus Q67897 RHBV
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Rice hoja blanca tenuivirus RHBV Q67897 RHBV
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells Rice hoja blanca tenuivirus ?
-
 ?
additional information the NS3 protein of Rice hoja blanca virus is an RNA silencing suppressor, RSS, that exclusively binds to small dsRNA molecules. This plant viral RSS lacks interferon antagonistic activity, yet it is able to substitute the RSS function of the Tat protein of Human immunodeficiency virus type 1 based on the sequestration of small dsRNA. NS3 is able to inhibit endogenous miRNA action in mammalian cells Rice hoja blanca tenuivirus RHBV ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, recombinant NS3, SDS-PAGE, x * 109000, recombinant MBP-fusion NS3 protein, SDS-PAGE Rice hoja blanca tenuivirus

Synonyms

Synonyms Comment Organism
non-structural protein 3 protein
-
 Rice hoja blanca tenuivirus
NS3 protein
-
 Rice hoja blanca tenuivirus