Cloned (Comment) | Organism |
---|---|
gene TaRH1, DNA and amino acid sequence determination and analysis, phylogenetic tree and analysis, quantitative real-time PCR enzyme expression analysis, the gene encoding the enzyme is differentially expressed under both biotic and several abiotic stresses, TaRH1-catalysed unwinding of duplex RNA following recombinant His-tagged TaRH1 expression in Escherichia coli strain BL21(DE3) | Triticum aestivum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Triticum aestivum | - |
ADP + phosphate | - |
? | |
additional information | Triticum aestivum | TaRH1-catalysed unwinding of duplex RNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | N0E6R8 | stress-responsive wheat | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged TaRH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Triticum aestivum | ADP + phosphate | - |
? | |
additional information | TaRH1-catalysed unwinding of duplex RNA | Triticum aestivum | ? | - |
? | |
additional information | ATP-dependent unwinding of duplex RNA in vitro by TaRH1 | Triticum aestivum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DEAD-box RNA helicase | - |
Triticum aestivum |
TaRH1 | - |
Triticum aestivum |
Triticum aestivum RNA helicase | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
ATP-dependent RNA unwinding assay at | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
ATP-dependent RNA unwinding assay at | Triticum aestivum |
Organism | Comment | Expression |
---|---|---|
Triticum aestivum | the gene encoding the enzyme is differentially expressed under both biotic and several abiotic stresses | additional information |
General Information | Comment | Organism |
---|---|---|
evolution | the putative ATP-dependent RNA helicase and is a member of the DEAD-box family of SF2, superfamily 2, phylogenetic tree and analysis | Triticum aestivum |
physiological function | DEAD-box RNA helicases play important roles in all types of processes in RNA metabolism. TaRH1 gene may participate in the plant stress response | Triticum aestivum |