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3.6.4.13: RNA helicase

This is an abbreviated version!
For detailed information about RNA helicase, go to the full flat file.

Word Map on EC 3.6.4.13

Reaction

a nucleoside triphosphate
+
H2O
=
a nucleoside diphosphate
 +
phosphate

Synonyms

1a NTPase/helicase, Aquarius, AtHELPS, ATP-dependent helicase, ATP-dependent RNA helicase, ATP-dependent RNA helicase DDX3X, ATP-dependent RNA helicase DDX5, ATP/dATP-dependent RNA helicase, ATPase, ATPase/helicase, ATPase/RNA helicase, AtRH3, AtRH7, bel, BmL3-helicase, BMV 1a protein, BRR2, Brr2 RNA helicase, Brr2p, Cbu_0670, ChlR1 helicase, cold-shock DEAD-box protein A, CrhR, CsdA, CshA, CTHT_0005780, CTHT_0009470, DBP2, DbpA, DDX17, DDX19, DDX19B, DDX21, DDX21 RNA helicase, DDX25, DDX27, DDX3, DDX3X, DDX3Y, DDX4, DDX5, DDX58, DeaD, DEAD box helicase, DEAD box RNA helicase, DEAD-box ATP-dependent RNA helicase CshA, DEAD-box helicase, DEAD-box protein DED1, DEAD-box RNA helicase, DEAD-box rRNA helicase, DEAH box protein 34, DEAH-box protein 2, DEAH-box RNA helicase, DEAH/RHA RNA helicase, DED1, DENV NS3H, DEx(H/D)RNA helicase, DExD/H box RNA helicase, DEXD/H-box RNA helicase, dexh helicase, DExH protein RNA helicase A, DExH/D-Box protein RNA helicase A, DExH/D-box RNA helicase, DHX34, DHX36, DHX8, DHX9, Dhx9/RNA helicase A, DNA/RNA-dependent ATPase, EhDEAD1, EhDEAD1 RNA helicase, eIF4A, eIF4A helicase, eIF4AIII, eukaryotic initiation factor eIF 4A, frequency-interacting RNA helicase, FRH, FRQ-interacting RNA helicase, gonadotropin-regulated testicular RNA helicase, GRTH, GRTH/DDX25, HCV NS3 helicase, HEL-1, helicase, helicase 1, helicase B, helicase/nucleoside triphosphatase, HRpA, IBP160, increased size exclusion limit2, intron-binding protein 160, ISE2, KOKV helicase, Lmo1722, mitochondrial DEAD-box RNA helicase, Mss116p, mtr4, Mtr4p, NA-helicase, non structural protein 3, non-structural 3, non-structural protein 3, non-structural protein 3 protein, nonstructural protein 3, NPH-II, NS3, NS3 ATPase/helicase, NS3 helicase, NS3 NTPase/helicase, NS3 protein, NTPase/helicase, nucleoside 5'-triphosphatase, nucleoside triphosphatase/helicase, nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase, NWMN_1985, p54 RNA helicase, p68, p68 RNA helicase, P72, PH1280, pre-mRNA-splicing ATP-dependent RNA helicase 28, protein NS3, Prp28, Prp43, Prp5, RENT1, RH22, RHA, RhlB, RIG-I, Rm62, RNA DEAD-box helicase, RNA helicase, RNA helicase A, RNA helicase Aquarius, RNA helicase BELLE, RNA helicase CrhR, RNA helicase CsdA, RNA helicase CshA, RNA helicase DDX17, RNA helicase DDX27, RNA helicase DDX3, RNA helicase Ddx39, RNA helicase DDX3X, RNA helicase DDX5, RNA helicase DDX6, RNA helicase DeaD, RNA helicase DHX34, RNA helicase DHX8, RNA helicase DHX9, RNA helicase HEL-1, RNA helicase Hera, RNA helicase ISE2, RNA Helicase p68, RNA helicase RHAU, RNA helicase UPF1, RNA splicing effector, RNA-dependent ATPase, RNA-dependent NTPase/helicase, RNA-helicase, RTPase, SA1885, Ski2-like helicase, slr0083, SNRNP200, spliceosomal DEAH-box RNA helicase, spliceosomal RNA helicase, SpolvlgA, Supv3L1, TaRH1, TGBp1 NTPase/helicase domain, Tk-DeaD, Triticum aestivum RNA helicase, Upf1, UPF1_1, UPF1_2, Vasa, VRH1, YxiN, ZmRH3, ZmRH3A, ZmRH3B

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.13 RNA helicase

Purification

Purification on EC 3.6.4.13 - RNA helicase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration
gel filtration, recombinant nonstructural protein 3
-
gel filtration, recombinant protein
gel filtration, recombinant protein, soluble form
-
gel filtration, SDS-PAGE
GSH Sepharose chromatography and Superdex 75 gel filtration
Thermochaetoides thermophila
recombinant C-terminally His-tagged and N-terminally calmodulin-binding-protein-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Rosetta by nickel and calmodulin affinity chromatography
recombinant C-terminally His-tagged truncated NS3 NTPase/helicase domain from Escherichia coli by nickel affinity chromatography
-
recombinant CBP- and His-tagged wild-type and mutant isozymes from Escherichia coli strain BL21(DE3) by nickel affinity and calmodulin affinity chromatography, followed by dialysis
recombinant enzyme
recombinant FLAG-Strep-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by two steps of affinity chromatography, recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography
recombinant GST-fusion wild-type and mutant enzymes from Escherichia coli strain C41(DE3) by glutathione affinity chromatography
-
recombinant His-tag C-terminal domain of NS3 protein from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged catalytic domain of the NS3 helicase domain from dengue virus serotype 4 from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme by nickel affinity chromatography and anion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli by ammonium sulfate fractionation, nickel affinity chromatography, and gel filtration
recombinant His-tagged enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity and heparin affinity chromatography
recombinant His-tagged enzyme N-terminal RecA-like domain 1 from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged TaRH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant RhlB and RNaseE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged wild-type and truncated mutant enzymes from Escherichia coli strain BL21(pLysS) by nickel affinity chromatography and dialysis
recombinant His10-tagged Arg-rich amino acid motif HCV1487-1500, complete domain 2, and domain 2 lacking the flexible loop from Escherichia coli strain BL21(DE3)
recombinant His10-tagged Brr2 fragments from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration
recombinant His6-GST-tagged full-length DHX8 and truncated mutant DHX8DELTA547 from Sf9 insect cells by nickel affinity and heparin affinity chromatography, and gel filtration
recombinant His6-tagged enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, tag cleavage by TEV protease, follwed by dialysis, another step of nickel affinity chromatography, and ultrafiltration of the flow-through
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
EF409381
recombinant N-terminally His-tagged wild-type and engineered enzymes from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant N-terminally His6-tagged mutant enzymes from HEK293E cells by nickel affinity chromatography
recombinant protein, gel filtration, SDS-PAGE
-
recombinant soluble His6-tagged C-terminal portion of NS3 in Escherichia coli by nickel affinity chromatography and gel filtration
-
recombinant wild-type and N-terminally truncated enzyme from Escherichia coli
-
recombinant wild-type and truncated mutant enzymes from Escherichia coli
truncated and full-length complexes between nonstructural protein 3 (NS3) and nonstructural protein 4A (NS4), NS3-4A complex purifies as two separable proteins, gel filtration, SDS-PAGE
-
using affinity chromatography
-
using Ni-NTA chromatography