Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in HEK293e cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K235A | site-directed mutagenesis of a conserved Lys residue in RNA binding domain dsRBD2, the mutation does not prevent purified full-length RNA helicase A from binding and unwinding duplex RNA in vitro, but efficiently inhibits RNA helicase A-stimulated HIV-1 RNA metabolism including the accumulation of viral mRNA and tRNALys3 annealing to viral RNA | Homo sapiens |
K236A | site-directed mutagenesis of a conserved Lys residue in RNA binding domain dsRBD2, the mutation does not prevent purified full-length RNA helicase A from binding and unwinding duplex RNA in vitro, but efficiently inhibits RNA helicase A-stimulated HIV-1 RNA metabolism including the accumulation of viral mRNA and tRNALys3 annealing to viral RNA | Homo sapiens |
K54A | site-directed mutagenesis of a conserved Lys residue in RNA binding domain dsRBD1, the mutation does not prevent purified full-length RNA helicase A from binding and unwinding duplex RNA in vitro, but efficiently inhibits RNA helicase A-stimulated HIV-1 RNA metabolism including the accumulation of viral mRNA and tRNALys3 annealing to viral RNA | Homo sapiens |
K55A | site-directed mutagenesis of a conserved Lys residue in RNA binding domain dsRBD1, the mutation does not prevent purified full-length RNA helicase A from binding and unwinding duplex RNA in vitro, but efficiently inhibits RNA helicase A-stimulated HIV-1 RNA metabolism including the accumulation of viral mRNA and tRNALys3 annealing to viral RNA | Homo sapiens |
additional information | wild-type and mutant enzymes in vitro RNA binding and unwinding or in the cell during HIV-1 production during RNA helicase A-RNA interaction and RNA helicase A-stimulated viral RNA processes, overview. The mutations do not prevent RNA helicase A from binding to HIV-1 RNA in vitro aswell, but dramatically reduce RNA helicase A-HIV-1 RNA interaction in the cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | while being mainly found in the nucleus, enzyme RHA can shuttle back and forth between the nucleus and the cytoplasm | Homo sapiens | 5737 | - |
nucleus | while being mainly found in the nucleus, enzyme RHA can shuttle back and forth between the nucleus and the cytoplasm | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
additional information | Homo sapiens | wild-type and mutant enzymes in vitro RNA binding and unwinding or in the cell during HIV-1 production during RNA helicase A-RNA interaction and RNA helicase A-stimulated viral RNA processes, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q08211 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged mutant enzymes from HEK293E cells by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
additional information | wild-type and mutant enzymes in vitro RNA binding and unwinding or in the cell during HIV-1 production during RNA helicase A-RNA interaction and RNA helicase A-stimulated viral RNA processes, overview | Homo sapiens | ? | - |
? | |
additional information | wild-type and mutant enzymes in vitro RNA binding and unwinding or in the cell during HIV-1 production during RNA helicase A-RNA interaction and RNA helicase A-stimulated viral RNA processes, overview. Ability of wild-type and mutant RHAs to stimulate the accumulation of HIV-1 mRNAs | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHX9 | - |
Homo sapiens |
RHA | - |
Homo sapiens |
RNA helicase A | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | RNA helicase A contains two double-stranded RNA binding domains (dsRBD1 and dsRBD2) at the N-terminus. Each dsRBD contains two invariant lysine residues critical for the binding of isolated dsRBDs to RNA. The conserved lysine residues of dsRBDs play critical roles in the promotion of HIV-1 production by RNA helicase A | Homo sapiens |
physiological function | RNA helicase A is a member of DExH-box RNA helicases, and has been reported to promote replication of a number of viruses, such as hepatitis C virus, foot-and mouth disease virus, influenza A virus, and HIV-1. The enzyme regulates a variety of RNA metabolism processes including HIV-1 replication | Homo sapiens |