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3.5.1.28: N-acetylmuramoyl-L-alanine amidase

This is an abbreviated version!
For detailed information about N-acetylmuramoyl-L-alanine amidase, go to the full flat file.

Word Map on EC 3.5.1.28

Reaction

GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
+
H2O
=
N-acetylglucosaminyl-N-acetylmuramic acid
 +
L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala

Synonyms

1,6-anhydro-N-acetylmuramic acid-L-alanine amidase, 1,6-anhydro-N-acetylmuramyl-L-alanine amidase, acetylmuramoyl-alanine amidase, acetylmuramyl-alanine amidase, acetylmuramyl-L-alanine amidase, AMI1, AmiA, AmiB, AmiC, AmiC2, AmiD, amidase 3, amidase-hydrolase, AmiE, AmpD, anhydroMurNAc-L-Ala amidase, AtlE, Autolysin, autolysin E, bacteriophage phiGVE2 amidase, cell separation amidase, cell wall amidase, Cell wall hydrolase, CwhA, CwlB protein, cwlC, CwlJ1, EC 3.4.17.7, EC 3.4.19.10, endolysin, GC-AmiC, GRCS_0011, LysBPS13, lysostaphin, LysSA97, LytA, LytA-like N-acetylmuramoyl-L-alanine amidase, LytAB6, LytAHER, Lytic amidase, Lytmu1/6, LytN, More, MSMEG_6281, Mtb peptidoglycan amidase, Mucopeptide aminohydrolase, murein hydrolase, MurnAc-lAA, Nacetylmuramoyl-L-alanine amidase activity, N-acetyl muramyl-L-alanine amidase, N-acetyl-muramoyl-L-alanine amidase, N-acetylmuramic acid L-alanine amidase, N-acetylmuramoyl-L-alanine amidase, N-acetylmuramoyl-l-alanine amidase B, N-acetylmuramoyl-L-alanine amidase type I, N-acetylmuramoyl-L-alanine amidase type II, N-acetylmuramyl-L-alanine amidase, N-acetylmuramylalanine amidase, N-acylmuramyl-L-alanine amidase, NAM-amidase, NAMLA amidase, NAMLAA, Npun_F1846, ORFL3, peptidoglycan amidase, peptidoglycan aminohydrolase, Peptidoglycan hydrolase, peptidoglycan recognition protein 2, peptidoglycan recognition protein SC1a, peptidoglycan recognition protein-L, PG amidase, PGLYRP2, PGRP, PGRP-L, PGRP-S4, PGRP-SB1, phage endolysin, phi26F_gp22, phiCP26F endolysin, phiGVE2 endolysin, PlyCP26F, PlyCP39O, PlyCpAmi, PlyGRCS, PlyPl23, RC0497, RS15875, Rv3717, SA97_036, short peptidoglycan recognition protein 4, Skl, Sle1, SleC, spore cortex lytic enzyme, T3 lysozyme, T7 lysozyme

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.28 N-acetylmuramoyl-L-alanine amidase

Cloned

Cloned on EC 3.5.1.28 - N-acetylmuramoyl-L-alanine amidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ampD gene, expression in Escherichia coli
-
Escherichia coli
expressed in Escherichia coli BL21 Star(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells as a cytoplasmic N-terminal His10-tagged fusion protein
-
expressed in Escherichia coli LMG194 and B180 cells
expression in COS-7 cells and in HepG2/C3A human hepatoblastoma cells
expression in Escherichia coli
gene amiB, DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene amiC, functional recombinant expression of GST-tagged wild-type enzyme and isolated catalytic domain in Escherichia coli strain MG165
gene amiC, recombinant expression of N-terminally His6-tagged enzyme AmiC residues 29-159 fused to GB1 in Escherichia coli strain BL21(DE3), cells are grown in M9 minimal media containing 13C6-glucose and 15NH4Cl as carbon and nitrogen sources, respectively
gene amiC, sequence comparisons, recombinant expression of C-terminally HA-tagged or His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
-
gene cwlB, the gene and upstream gene cwlA form one transcriptional unit, DNA and amino acid sequence determination and analysis, sequence comparison. Transcription of the operon is directed by a promoter, PcwlA, which is located upstream from the cwlA gene and that the transcription start site is a single 5'-end nucleotide residue T located 25 nucleotides upstream from the cwlA translational start codon. The activity of PcwlA is controlled by sigmaK. Subcloning and gene modifications in Escherichia coli strains TG1 and SCS110, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene cwlC or RS15875, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), cwlC is transcribed as a monocistronic unit and its expression is dependent on sporulation sigma factor K
-
gene cwlD, DNA and amino acid sequence determination and analysis from strains CP26, CP39, CP509, CP297, CP697, CP726, CP776, CP1036, and CP1113, genotyping, recombinant expression in Escherichia coli strain Rosetta 2 (DE3), cloning in Escherichia coli strain DH5alpha
gene cwlJ1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS
A0A1J9W3F9
gene IBBPl23_21, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged in Escherichia coli strains JM109 and BL21(DE3)
gene lytAB6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B
gene lytAHER, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B
gene pglyrp2, DNA and amino acid sequence determination and analysis, no alternative splice forms, transient expression in COS-7 and HEK-293 cells, expression of wild-type and truncated mutant enzymes in Escherichia coli as inclusion bodies
gene PGRP-SB1, DNA and amino acid sequence determination and analysis, expression of His6-tagged 128 C-terminal residues and of his6-tagged 180 N-terminal residues in Escherichia coli strain XL-1I Blue
gene PGRP-SB1, expression of His6-tagged wild-type and mutant enzyme
gene RC0497, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Rv3717, expression of recombinant GST-tagged enzyme in Escherichia coli strain BL-21 Codon Plus
gene SA97_036, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged cell wall binding domain CBD fused to GFP in Escherichia coli strain BL21(DE3)
gene skl, expression in Escherichia coli strain DH10B
-
gene sle1, DNA and amino acid sequence determination and analysis, expression of His6-tagged 128 C-terminal residues and of His6-tagged 180 N-terminal residues in Escherichia coli strain XL-1I Blue
-
genen amiC, functional recombinant expression in Escherichia coli, enzyme AmiC complements and restores cell separation defects seen in Escherichia coli amidase mutants
lytA gene, expression in Escherichia coli RB791
overexpression in Escherichia coli
-
PGRP-S4, DNA and amino acid sequence determination analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21, real-time PCR analysis enzyme expression analysis
-
sequence comparisons, recombinant expression of His-tagged wild-type and mutant endolysins in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL10 GOLD
synthesis of a gene, codon optimized for Escherichia coli expression, that encodes the catalytic domain of the bacteriophage phiGVE2 amidase and the cell-wall binding (CWB) domain of the endolysin encoded by the genome of phiCP26F, recombinant expression of His-tagged chimeric enzyme PlyGVE2CpCWB in Escherichia coli strain BL21(DE3)