Literature summary for 3.5.1.28 extracted from

Yang, J.; Peng, Q.; Chen, Z.; Deng, C.; Shu, C.; Zhang, J.; Huang, D.; Song, F.
Transcriptional regulation and characteristics of a novel N-acetylmuramoyl-L-alanine amidase gene involved in Bacillus thuringiensis mother cell lysis (2013), J. Bacteriol., 195, 2887-2897.

Cloned(Commentary)

Cloned (Comment)	Organism
gene cwlB, the gene and upstream gene cwlA form one transcriptional unit, DNA and amino acid sequence determination and analysis, sequence comparison. Transcription of the operon is directed by a promoter, PcwlA, which is located upstream from the cwlA gene and that the transcription start site is a single 5'-end nucleotide residue T located 25 nucleotides upstream from the cwlA translational start codon. The activity of PcwlA is controlled by sigmaK. Subcloning and gene modifications in Escherichia coli strains TG1 and SCS110, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Bacillus thuringiensis

Cloned (Comment)

Organism

gene cwlB, the gene and upstream gene cwlA form one transcriptional unit, DNA and amino acid sequence determination and analysis, sequence comparison. Transcription of the operon is directed by a promoter, PcwlA, which is located upstream from the cwlA gene and that the transcription start site is a single 5'-end nucleotide residue T located 25 nucleotides upstream from the cwlA translational start codon. The activity of PcwlA is controlled by sigmaK. Subcloning and gene modifications in Escherichia coli strains TG1 and SCS110, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Bacillus thuringiensis

Protein Variants

Protein Variants	Comment	Organism
additional information	gene cwlB and upstream gene cwlA form one transcriptional unit, construction of the cwlA, cwlB, and cwlAB deletion mutants and genetic complementation of the cwlB deletion mutant	Bacillus thuringiensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell envelope	-	Bacillus thuringiensis	30313	-
cell wall	-	Bacillus thuringiensis	5618	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24500	-	x * 24500, recombinant His-tagged enzyme, SDS-PAGE	Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Bacillus thuringiensis	determination of the sites of Bacillus thuringiensis cell wall cleavage by the enzyme, overview	?	-	?
additional information	Bacillus thuringiensis HD73	determination of the sites of Bacillus thuringiensis cell wall cleavage by the enzyme, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus thuringiensis	-	subsp. kurstaki, orf bt2494, gene cwlB	-
Bacillus thuringiensis HD73	-	subsp. kurstaki, orf bt2494, gene cwlB	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) cell walls by sonication and nickel affinity chromatography	Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	determination of the sites of Bacillus thuringiensis cell wall cleavage by the enzyme, overview	Bacillus thuringiensis	?	-	?
additional information	the recombinant enzyme shows cell wall binding ability and amidase activity in vitro	Bacillus thuringiensis	?	-	?
additional information	determination of the sites of Bacillus thuringiensis cell wall cleavage by the enzyme, overview	Bacillus thuringiensis HD73	?	-	?
additional information	the recombinant enzyme shows cell wall binding ability and amidase activity in vitro	Bacillus thuringiensis HD73	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 24500, recombinant His-tagged enzyme, SDS-PAGE	Bacillus thuringiensis

Synonyms

Synonyms	Comment	Organism
CwlB protein	-	Bacillus thuringiensis
MurnAc-lAA	-	Bacillus thuringiensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bacillus thuringiensis

General Information

General Information	Comment	Organism
malfunction	mutation of gene cwlB delays spore release compared to the timing of spore release in the wild-type strain	Bacillus thuringiensis
physiological function	the enzyme is involved in mother cell lysis, determination of the sites of cell wall cleavage by the enzyme, overview	Bacillus thuringiensis