Literature summary for 3.5.1.28 extracted from

Zhang, Y.; Van der Fits, L.; Voerman, J.S.; Melief, M.; Laman, J.D.; Wang, M.; Wang, H.; Wang, M.; Li, X.; Walls, C.D.; Gupta, D.; Dziarski, R.
Identification of serum N-acetylmuramoyl-L-alanine amidase as liver peptidoglycan recognition protein 2 (2005), Biochim. Biophys. Acta, 1752, 34-46.

Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment)	Organism
gene pglyrp2, DNA and amino acid sequence determination and analysis, no alternative splice forms, transient expression in COS-7 and HEK-293 cells, expression of wild-type and truncated mutant enzymes in Escherichia coli as inclusion bodies	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of deletion mutants DELTA1-322, DELTA323-555, and DELTA474-555	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	serum	Homo sapiens	-	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q96PD5	PGLYRP2; gene pglyrp2	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Homo sapiens
phosphoprotein	the enzyme contains a phosphorylation site at S218	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from liver by gel filtration, and immunoaffinity chromatography, native serum enzyme by immunoaffinity chromatography solubilization and purification of recombinant wild-type and truncated mutant enzymes from Escherichia coli inclusion bodies by treatment with 6 M urea and nickel affinity chromatography under denaturing conditions	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
solubilization and purification of recombinant wild-type and truncated mutant enzymes from Escherichia coli inclusion bodies by treatment with 6 M urea and nickel affinity chromatography under denaturing conditions	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-
serum	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetylmuramoyl-L-alanine + H2O	the enzyme hydrolyzes bacterial peptidoglycan	Homo sapiens	N-acetylmuramate + L-alanine	-	?

Subunits

Subunits	Comment	Organism
More	peptide mapping and immunospecificity analysis of enzyme proteins, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
NAMLAA	-	Homo sapiens
peptidoglycan recognition protein 2	-	Homo sapiens
PGLYRP2	-	Homo sapiens

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Release 2023.1 (January 2023)