Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.28: N-acetylmuramoyl-L-alanine amidase

This is an abbreviated version!
For detailed information about N-acetylmuramoyl-L-alanine amidase, go to the full flat file.

Word Map on EC 3.5.1.28

Reaction

GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
+
H2O
=
N-acetylglucosaminyl-N-acetylmuramic acid
 +
L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala

Synonyms

1,6-anhydro-N-acetylmuramic acid-L-alanine amidase, 1,6-anhydro-N-acetylmuramyl-L-alanine amidase, acetylmuramoyl-alanine amidase, acetylmuramyl-alanine amidase, acetylmuramyl-L-alanine amidase, AMI1, AmiA, AmiB, AmiC, AmiC2, AmiD, amidase 3, amidase-hydrolase, AmiE, AmpD, anhydroMurNAc-L-Ala amidase, AtlE, Autolysin, autolysin E, bacteriophage phiGVE2 amidase, cell separation amidase, cell wall amidase, Cell wall hydrolase, CwhA, CwlB protein, cwlC, CwlJ1, EC 3.4.17.7, EC 3.4.19.10, endolysin, GC-AmiC, GRCS_0011, LysBPS13, lysostaphin, LysSA97, LytA, LytA-like N-acetylmuramoyl-L-alanine amidase, LytAB6, LytAHER, Lytic amidase, Lytmu1/6, LytN, More, MSMEG_6281, Mtb peptidoglycan amidase, Mucopeptide aminohydrolase, murein hydrolase, MurnAc-lAA, Nacetylmuramoyl-L-alanine amidase activity, N-acetyl muramyl-L-alanine amidase, N-acetyl-muramoyl-L-alanine amidase, N-acetylmuramic acid L-alanine amidase, N-acetylmuramoyl-L-alanine amidase, N-acetylmuramoyl-l-alanine amidase B, N-acetylmuramoyl-L-alanine amidase type I, N-acetylmuramoyl-L-alanine amidase type II, N-acetylmuramyl-L-alanine amidase, N-acetylmuramylalanine amidase, N-acylmuramyl-L-alanine amidase, NAM-amidase, NAMLA amidase, NAMLAA, Npun_F1846, ORFL3, peptidoglycan amidase, peptidoglycan aminohydrolase, Peptidoglycan hydrolase, peptidoglycan recognition protein 2, peptidoglycan recognition protein SC1a, peptidoglycan recognition protein-L, PG amidase, PGLYRP2, PGRP, PGRP-L, PGRP-S4, PGRP-SB1, phage endolysin, phi26F_gp22, phiCP26F endolysin, phiGVE2 endolysin, PlyCP26F, PlyCP39O, PlyCpAmi, PlyGRCS, PlyPl23, RC0497, RS15875, Rv3717, SA97_036, short peptidoglycan recognition protein 4, Skl, Sle1, SleC, spore cortex lytic enzyme, T3 lysozyme, T7 lysozyme

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.28 N-acetylmuramoyl-L-alanine amidase

Purification

Purification on EC 3.5.1.28 - N-acetylmuramoyl-L-alanine amidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
10-15fold
354fold
-
56fold
-
7000-10000fold
-
739fold
-
combination of affinity and cation-exchange chromatographies
-
DEAE-cellulose column chromatography
-
from achromopeptidase
gene PGRP-SB1, recombinant His6-tagged wild-type and mutant enzyme by nickel affinity chromatography
HiTrap column chromatography, gel filtration
-
native enzyme 307.7fold from cell culture medium by dye-ligand affinity chromatography, the flow through of the step is used in hydrophobic interaction chromatography
-
native enzyme from liver by gel filtration, and immunoaffinity chromatography, native serum enzyme by immunoaffinity chromatography solubilization and purification of recombinant wild-type and truncated mutant enzymes from Escherichia coli inclusion bodies by treatment with 6 M urea and nickel affinity chromatography under denaturing conditions
Ni-nitrilotriacetate resin column chromatography
Ni-NTA resin column chromatography
-
Ni-NTA Sepharose column chromatography, gel filtration
-
recombinant C-terminally His-tagged enzyme 38fold from Escherichia coli by nickel affinity and cation exchange chromatography to 98% homogeneity
-
recombinant C-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, dialysis, and tag cleavage via TEV protease, followed by another step of nickel affinity chromatography
-
recombinant GST-tagged enzyme from Escherichia coli strain BL-21 Codon Plus by glutathione affinity chromatography and tag cleavage, followed by a combined glutathione affinity/anion exchange chromatography step, and a gel filtration step
recombinant His-tagged chimeric enzyme PlyGVE2CpCWB from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) cell walls by sonication and nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant endolysins from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
A0A1J9W3F9
recombinant His6-tagged from Escherichia coli strains JM109 and BL21(DE3) by nickel affinity chromatography and ultrafiltration
recombinant N-terminally His-tagged cell wall binding domain CBD fused to GFP from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to near homogeneity
recombinant N-terminally His6-tagged enzyme AmiC residues 29-159 fused to GB1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by His6-TEV protease, and again nickel affinity chromatography
recombinant phage enzyme from Escherichia coli
recombinant wild-type and mutant AmpD
-
S-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S100 gel filtration
two methods
-