Cloned (Comment) | Organism |
---|---|
gene lytAB6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B | Streptococcus mitis |
gene lytAHER, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B | Streptococcus mitis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
choline | IC50: 0.4 mM; IC50: 2 mM | Streptococcus mitis | |
deoxycholate | at 1%; at 1% | Streptococcus mitis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme posseses transmembrane segments | Streptococcus mitis | 16020 | - |
membrane | the enzyme possesses transmembrane segments | Streptococcus mitis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36782 | - |
x * 36782, sequence calculation | Streptococcus mitis |
36919 | - |
x * 36919, sequence calculation | Streptococcus mitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptococcus mitis | the enzyme performs cell wall lysis | ? | - |
? | |
additional information | Streptococcus mitis HER1055 | the enzyme performs cell wall lysis | ? | - |
? | |
additional information | Streptococcus mitis B6 | the enzyme performs cell wall lysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mitis | Q706F5 | Streptococcus mitis bacteriophage PHIB6 | - |
Streptococcus mitis | Q706F9 | Streptococcus mitis bacteriophage PHIHER; isolated from Streptococcus mitis strain HER1055 | - |
Streptococcus mitis B6 | Q706F5 | Streptococcus mitis bacteriophage PHIB6 | - |
Streptococcus mitis HER1055 | Q706F9 | Streptococcus mitis bacteriophage PHIHER; isolated from Streptococcus mitis strain HER1055 | - |
Purification (Comment) | Organism |
---|---|
recombinant phage enzyme from Escherichia coli | Streptococcus mitis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000044 | - |
- |
Streptococcus mitis |
0.0000046 | - |
- |
Streptococcus mitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme performs cell wall lysis | Streptococcus mitis | ? | - |
? | |
additional information | the enzyme performs cell wall lysis | Streptococcus mitis HER1055 | ? | - |
? | |
additional information | the enzyme performs cell wall lysis | Streptococcus mitis B6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36782, sequence calculation | Streptococcus mitis |
? | x * 36919, sequence calculation | Streptococcus mitis |
Synonyms | Comment | Organism |
---|---|---|
LytA-like N-acetylmuramoyl-L-alanine amidase | - |
Streptococcus mitis |
LytAB6 | - |
Streptococcus mitis |
LytAHER | - |
Streptococcus mitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Streptococcus mitis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.4 | - |
IC50: 0.4 mM | Streptococcus mitis | choline | |
2 | - |
IC50: 2 mM | Streptococcus mitis | choline |