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3.5.1.28: N-acetylmuramoyl-L-alanine amidase

This is an abbreviated version!
For detailed information about N-acetylmuramoyl-L-alanine amidase, go to the full flat file.

Word Map on EC 3.5.1.28

Reaction

GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
+
H2O
=
N-acetylglucosaminyl-N-acetylmuramic acid
+
L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala

Synonyms

1,6-anhydro-N-acetylmuramic acid-L-alanine amidase, 1,6-anhydro-N-acetylmuramyl-L-alanine amidase, acetylmuramoyl-alanine amidase, acetylmuramyl-alanine amidase, acetylmuramyl-L-alanine amidase, AMI1, AmiA, AmiB, AmiC, AmiC2, AmiD, amidase 3, amidase-hydrolase, AmiE, AmpD, anhydroMurNAc-L-Ala amidase, AtlE, Autolysin, autolysin E, bacteriophage phiGVE2 amidase, cell separation amidase, cell wall amidase, Cell wall hydrolase, CwhA, CwlB protein, cwlC, CwlJ1, EC 3.4.17.7, EC 3.4.19.10, endolysin, GC-AmiC, GRCS_0011, LysBPS13, lysostaphin, LysSA97, LytA, LytA-like N-acetylmuramoyl-L-alanine amidase, LytAB6, LytAHER, Lytic amidase, Lytmu1/6, LytN, More, MSMEG_6281, Mtb peptidoglycan amidase, Mucopeptide aminohydrolase, murein hydrolase, MurnAc-lAA, Nacetylmuramoyl-L-alanine amidase activity, N-acetyl muramyl-L-alanine amidase, N-acetyl-muramoyl-L-alanine amidase, N-acetylmuramic acid L-alanine amidase, N-acetylmuramoyl-L-alanine amidase, N-acetylmuramoyl-l-alanine amidase B, N-acetylmuramoyl-L-alanine amidase type I, N-acetylmuramoyl-L-alanine amidase type II, N-acetylmuramyl-L-alanine amidase, N-acetylmuramylalanine amidase, N-acylmuramyl-L-alanine amidase, NAM-amidase, NAMLA amidase, NAMLAA, Npun_F1846, ORFL3, peptidoglycan amidase, peptidoglycan aminohydrolase, Peptidoglycan hydrolase, peptidoglycan recognition protein 2, peptidoglycan recognition protein SC1a, peptidoglycan recognition protein-L, PG amidase, PGLYRP2, PGRP, PGRP-L, PGRP-S4, PGRP-SB1, phage endolysin, phi26F_gp22, phiCP26F endolysin, phiGVE2 endolysin, PlyCP26F, PlyCP39O, PlyCpAmi, PlyGRCS, PlyPl23, RC0497, RS15875, Rv3717, SA97_036, short peptidoglycan recognition protein 4, Skl, Sle1, SleC, spore cortex lytic enzyme, T3 lysozyme, T7 lysozyme

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.28 N-acetylmuramoyl-L-alanine amidase

Molecular Weight

Molecular Weight on EC 3.5.1.28 - N-acetylmuramoyl-L-alanine amidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
-
gel filtration, fraction I
120000
-
gel filtration, (Sephadex)
130000
-
gel filtration, (Sephacryl)
135000
-
native gradient PAGE, gel filtration
148000
-
x * 148000, multifunctional AtlE
18500
-
gel filtration, SDS-PAGE
19396
1 * 19396, sequence calculation
20000
-
SDS-PAGE
20847
-
x * 20847, wild-type AmpD, mass spectrometry
21000
220000
-
gel filtration, fraction II
24500
-
x * 24500, recombinant His-tagged enzyme, SDS-PAGE
26000
-
x * 26000, SDS-PAGE
28000
-
x * 28000, SDS-PAGE
30000
-
x * 30000, SDS-PAGE
30000 - 40000
-
gel filtration
31130
x * 31130, calculated from amino acid sequence
32000
35000
35800
-
x * 32000, processed enzyme, SDS-PAGE x * 35800, unprocessed enzyme, sequence calculation
36000
Pneumococcal bacteriophage HB-3
-
x * 36000, SDS-PAGE
36600
2 * 36600, catalytically active homodimer, dimer interface
36782
x * 36782, sequence calculation
36919
x * 36919, sequence calculation
37500
-
x * 37500, SDS-PAGE
38969
1 * 40000, SDS-PAGE, 1 * 38969, sequence calculation
39000
41000
-
SDS-PAGE, gel filtration
47000
-
sucrose density gradient centrifugation
51000
-
SDS-PAGE
57000
-
1 * 57000 + 1 * 70000, SDS-PAGE
58000
-
SDS-PAGE
62000
-
sucrose density gradient centrifugation
62920
-
calculated from amino acid sequence
65000
N-acetylmuramoyl-L-alanine amidase domain of the bifunctional autolysin AtlL
70000
74000
-
2 * 74000, SDS-PAGE
75000
-
SDS-PAGE
800000 - 1000000
-
sucrose density gradient centrifugation, gel filtration
82000
-
gel filtration
additional information
-
the gene encodes a protein of 137 kDa which is exported and processed in the multiple autolysin bands seen