3.4.24.B16: protease lasA

This is an abbreviated version!
For detailed information about protease lasA, go to the full flat file.

Reaction

proteolytic degradation of proteins =

Synonyms

bacteriolytic enzyme, LasA, LasA endopeptidase, LasA protease, M23.002, Pseudomonas elastase, staphylolysin, staphylolytic enzyme

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.B16 protease lasA

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1	Activating Compound
1	AA Sequence
9	Application
1	CAS Registry Number
1	Cloned(Commentary)
1	Crystallization (Commentary)
3	Expression
3	General Information
19	Inhibitors
3	KM Value [mM]
10	Localization
3	Metals/Ions
14	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
26	Organism
2	pH Optimum
1	pI Value
6	Posttranslational Modification
8	Protein Variants
9	Purification (Commentary)
6	Reaction
8	Reaction Type
23	Reference
1	Specific Activity [micromol/min/mg]
1	Storage Stability
33	Substrates and Products (Substrate)
7	Subunits
44	Synonyms
1	Temperature Optimum [°C]
3	Turnover Number [1/s]

Reaction

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Reaction on EC 3.4.24.B16 - protease lasA

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Reaction Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

proteolytic degradation of proteins

6 entries

proteolytic degradation of proteins

-

-

-

-

proteolytic degradation of proteins

enzyme might be a modified serine protease with a His residue in the active site, specifically involved in degradation of elastin

Pseudomonas aeruginosa

-

653278

proteolytic degradation of proteins

His120 is critical for LasA activity

Pseudomonas aeruginosa

-

651625

proteolytic degradation of proteins

specific for Gly-Ala peptide bonds within Gly-Gly-Ala sequences in elastin, substrates that contain no Gly-Gly peptide bond, such as beta-casein, appear to be resistant to the enzyme

Pseudomonas aeruginosa

-

651880

proteolytic degradation of proteins

substrate carbonyl oxygen displaces Zn2+-bound water molecule 2, thereby enabling direct interaction with Zn2+ to polarize the peptide carbonyl bond, rendering it susceptible to nucleophilic attack by the second Zn2+-bound water molecule, water molecule1. Water molecule 1 is oriented by interactions with the Nepsilon atoms of both His120 and His81, while that with Zn2+ is weakened. Either of these residues is capable of abstracting a proton from water molecule 1, enabling its addition, as hydroxide, to the substrate carbonyl carbon to generate an oxyanion that is stabilized by bidentate co-ordination of Zn2+ and by interaction with the unprotonated His residue. Proton transfer from the histidine general base to the departing amide nitrogen facilitates cleavage of the peptide bond to generate a product complex

Pseudomonas aeruginosa

P14789

712754

proteolytic degradation of proteins

His120 is critical for LasA activity

Pseudomonas aeruginosa FRD1

-

-