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Literature summary for 3.4.24.B16 extracted from
- A substitution at His-120 in the LasA protease of Pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion (1996), J. Bacteriol., 178, 6608-6617.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | elastase enhances staphylolytic activity of LasA | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, overexpression of gene lasA in Escherichia coli strain JM109 in form of the pre-proLasA precursor protein | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H120A | oligonucleotide-directed mutagenesis, formation of the lasA5 allele, processing to a 20 kDa LasA-H120A protein and secretion to the medium occurs, but the mutant enzyme has no staphylolytic activity | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Trypsin | cleaves the enzyme | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | active processed 20 kDa enzyme, member of the beta-lytic endopeptidase family of extracellular bacterial proteases | Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination | Pseudomonas aeruginosa |
| 42000 | - |
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination | Pseudomonas aeruginosa |
| 45582 | - |
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Elastin + H2O | Pseudomonas aeruginosa | contributes to the pathogenesis of Pseudomonas aeruginosa | ? | - |
? |  |
| Elastin + H2O | Pseudomonas aeruginosa FRD1 | contributes to the pathogenesis of Pseudomonas aeruginosa | ? | - |
? | |
| additional information | Pseudomonas aeruginosa | enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells | ? | - |
? | |
| additional information | Pseudomonas aeruginosa FRD1 | enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells | ? | - |
? | |
| protein + H2O | Pseudomonas aeruginosa | - |
peptides | - |
? | |
| protein + H2O | Pseudomonas aeruginosa FRD1 | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa FRD1 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | no autoproteolysis, the 31 amino acid residues signal sequence of pre-proLasA is cleaved off by a signal peptidase, processing of the remaining 42 kDa proLasA by incubation with the culture filtrate of Pseudomonas aeruginosa or trypsin to the active 20 kDa LasA | Pseudomonas aeruginosa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from the culture supernatant | Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| proteolytic degradation of proteins | His120 is critical for LasA activity | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Elastin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Elastin + H2O | contributes to the pathogenesis of Pseudomonas aeruginosa | Pseudomonas aeruginosa | ? | - |
? | |
| Elastin + H2O | - |
Pseudomonas aeruginosa FRD1 | ? | - |
? | |
| Elastin + H2O | contributes to the pathogenesis of Pseudomonas aeruginosa | Pseudomonas aeruginosa FRD1 | ? | - |
? | |
| additional information | enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells | Pseudomonas aeruginosa FRD1 | ? | - |
? | |
| protein + H2O | - |
Pseudomonas aeruginosa | peptides | - |
? | |
| protein + H2O | - |
Pseudomonas aeruginosa FRD1 | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LasA | - |
Pseudomonas aeruginosa |
| LasA protease | - |
Pseudomonas aeruginosa |
| M23.002 | Merops-ID | Pseudomonas aeruginosa |
| staphylolysin | - |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Pseudomonas aeruginosa |
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