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proteolytic degradation of proteins
proteolytic degradation of proteins
-
-
-
-
proteolytic degradation of proteins
enzyme might be a modified serine protease with a His residue in the active site, specifically involved in degradation of elastin
-
proteolytic degradation of proteins
His120 is critical for LasA activity
-
proteolytic degradation of proteins
specific for Gly-Ala peptide bonds within Gly-Gly-Ala sequences in elastin, substrates that contain no Gly-Gly peptide bond, such as beta-casein, appear to be resistant to the enzyme
-
proteolytic degradation of proteins
substrate carbonyl oxygen displaces Zn2+-bound water molecule 2, thereby enabling direct interaction with Zn2+ to polarize the peptide carbonyl bond, rendering it susceptible to nucleophilic attack by the second Zn2+-bound water molecule, water molecule1. Water molecule 1 is oriented by interactions with the Nepsilon atoms of both His120 and His81, while that with Zn2+ is weakened. Either of these residues is capable of abstracting a proton from water molecule 1, enabling its addition, as hydroxide, to the substrate carbonyl carbon to generate an oxyanion that is stabilized by bidentate co-ordination of Zn2+ and by interaction with the unprotonated His residue. Proton transfer from the histidine general base to the departing amide nitrogen facilitates cleavage of the peptide bond to generate a product complex
proteolytic degradation of proteins
His120 is critical for LasA activity
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-
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4-(dimethylamino)azobenzene-4'-sulfonyl chloride-Leu-Gly-Gly-Gly-Ala-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O
?
-
-
-
-
?
beta-casein + H2O
beta-casein + 30 amino acid residues fragment
-
only 1 cleavage site: in the sequence NKKIEKFQphosphorylated-S between Lys and Ile, serine protease activity
-
?
LGGGA + H2O
Lys-Gly-Gly + Gly-Ala
-
best substrate
-
-
?
succinyl-Gly-Gly-Leu-4-nitroanilide + H2O
Leu-4-nitroanilide + succinyl-Gly-Gly
succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
Phe-4 nitroanilide + succinyl-Gly-Gly
tropoelastin + H2O
?
-
preferentially cleave at Gly-Gly-Ala
-
-
?
additional information
?
-
Elastin + H2O
?
-
-
-
?
Elastin + H2O
?
-
slow degradation
-
-
?
Elastin + H2O
?
-
cleavage of elastin by the enzyme possibly results in the unfolding or disruption of the complex elastin structure, thus providing more access to elastase and other proteases, elastolytic activity in absence of elastase, enzyme also enhances the elastolytic activity of elastase together with alkaline phophatase
-
?
Elastin + H2O
?
-
soluble recombinant and insoluble elastin
-
?
Elastin + H2O
?
-
contributes to the pathogenesis of Pseudomonas aeruginosa
-
?
Elastin + H2O
?
-
enzyme activates elastase, and the elastolytic activity of other proteases by cleaving elastin
-
?
Elastin + H2O
?
-
contributes to the pathogenesis of Pseudomonas aeruginosa
-
?
protein + H2O
peptides
-
-
-
?
protein + H2O
peptides
-
-
-
?
protein + H2O
peptides
-
-
-
?
protein + H2O
peptides
-
-
-
?
protein + H2O
peptides
-
-
-
?
protein + H2O
peptides
-
-
-
?
protein + H2O
peptides
-
-
-
?
protein + H2O
peptides
-
-
-
?
succinyl-Gly-Gly-Leu-4-nitroanilide + H2O
Leu-4-nitroanilide + succinyl-Gly-Gly
-
-
-
-
?
succinyl-Gly-Gly-Leu-4-nitroanilide + H2O
Leu-4-nitroanilide + succinyl-Gly-Gly
-
-
-
-
?
succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
Phe-4 nitroanilide + succinyl-Gly-Gly
-
better substrate than succinyl-Gly-Gly-Leu-4-nitroanilide
-
-
?
succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
Phe-4 nitroanilide + succinyl-Gly-Gly
-
better substrate than succinyl-Gly-Gly-Leu-4-nitroanilide
-
-
?
additional information
?
-
-
enzyme activates elastase, and the elastolytic activity of thermolysin, human neutrophil elastase, proteinase K, by interacting with the elastin substrate rather than the other enzyme
-
?
additional information
?
-
-
no activity with tosyl-Gly-Pro-Lys-4-nitroanilide, enzyme activates elastase, and the elastolytic, not the proteolytic, activity of thermolysin, human neutrophil elastase, proteinase K, by interacting with the elastin substrate rather than the other enzymes
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?
additional information
?
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enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells
-
?
additional information
?
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enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells
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?
additional information
?
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enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells by cleaving the peptidoglycan pentaglycine interpeptides
-
?
additional information
?
-
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no cleavage of beta-casein, does not bind chitin
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-
?
additional information
?
-
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no hydrolysis of 4-(dimethylamino)azobenzene-4'-sulfonyl chloride-Ala-Ala-Phe-Ala-5-(2-aminoethylamino)-1-naphthalenesulfonic acid
-
-
?
additional information
?
-
-
staphylolytic activity is pentaglycine in the peptidoglycan of Staphylococcus aureus
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-
?
additional information
?
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enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells
-
?
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Amyloidosis
A novel lysozyme mutation Phe57Ile associated with hereditary renal amyloidosis.
Bone Resorption
[Inhibition of plaque formation, caries development and alveolar bone resorption in hamsters by a bacteriolytic enzyme from Streptomyces globisporus (author's transl)]
Communicable Diseases
Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors.
Corneal Injuries
Pseudomonas aeruginosa LasA protease and corneal infections.
Cystic Fibrosis
Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain.
Dental Caries
Identification of a fibrinolytic enzyme by Bacillus vallismortis and its potential as a bacteriolytic enzyme against Streptococcus mutans
Dental Caries
Identification of a fibrinolytic enzyme by Bacillus vallismortis and its potential as a bacteriolytic enzyme against Streptococcus mutans.
Dental Plaque
[Effects of a bacteriolytic enzyme from Streptomyces globisporus on microflora of human dental plaque and tongue coating]
Dental Plaque
[Scanning electron microscope investigation of the in vitro effects of a bacteriolytic enzyme on human dental plaque]
Dental Plaque
[Scanning electron microscope study on the effects of a bacteriolytic enzyme derived from Streptomyces globisporus upon artificial dental plaque formed by cariogenic microorganisms (author's transl)]
Dental Plaque
[Scanning electron microscope study on the removal of artificial dental plaque treated with a bacteriolytic enzyme from Streptomyces globisporus--its synergistic effects with dextranase and lysozyme (author's transl)]
Diabetes Mellitus
Effect of risk factors and antirheumatic drugs on the proliferation of aortic wall cells.
Endophthalmitis
Evaluation of Pseudomonas aeruginosa staphylolysin (LasA protease) in the treatment of methicillin-resistant Staphylococcus aureus endophthalmitis in a rat model.
Eye Infections
Pseudomonas aeruginosa LasA protease and corneal infections.
Hypertension
Effect of risk factors and antirheumatic drugs on the proliferation of aortic wall cells.
Infections
Antibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections.
Infections
Contribution of proteases and LasR to the virulence of Pseudomonas aeruginosa during corneal infections.
Infections
Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors.
Infections
Inhibition of Quorum Sensing-Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa by Culture Extract from Novel Bacterial Species of Paenibacillus Using a Rat Model of Chronic Lung Infection.
Infections
Iron facilitates the RetS-Gac-Rsm cascade to inversely regulate protease IV (piv) expression via the sigma factor PvdS in Pseudomonas aeruginosa.
Infections
Positive correlation of algD transcription to lasB and lasA transcription by populations of Pseudomonas aeruginosa in the lungs of patients with cystic fibrosis.
Infections
Pseudomonas aeruginosa LasA protease and corneal infections.
Infections
Pseudomonas aeruginosa LasA protease in treatment of experimental staphylococcal keratitis.
Infections
Pseudomonas aeruginosa proteases and corneal virulence.
Infections
Serological assays against Staphylococcus aureus peptidoglycan, crude staphylococcal antigen and staphylolysin in the diagnosis of serious S. aureus infections.
Infections
THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI.
Infections
The transcriptional signatures of Sodalis glossinidius in the Glossina palpalis gambiensis flies negative for Trypanosoma brucei gambiense contrast with those of this symbiont in tsetse flies positive for the parasite: Possible involvement of a Sodalis-hosted prophage in fly Trypanosoma refractoriness?
Keratitis
Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors.
Keratitis
Pseudomonas aeruginosa LasA protease in treatment of experimental staphylococcal keratitis.
Keratitis
Staphylolysin is an effective therapeutic agent for Staphylococcus aureus experimental keratitis.
Leukemia
[Neutrophil phagocytic function and the anti-alpha staphylolysin blood levels in infectious complications in acute leukemia patients]
Measles
[Serum proteins and specific antibodies in a German and an alien population (author's transl)]
Staphylococcal Infections
Evaluation of activity of Recombinant Lysostaphin against isolates of Methicillin resistant Staphylococcus aureus from Indian hospitals.
Stomach Ulcer
Relation of fat degeneration induced by staphylolysin with inhibition of acute gastric ulcers in Shay rats.
Urinary Tract Infections
Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors.
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19000
-
x * 19000, SDS-PAGE
19963
x * 19963, electrospray mass spectrometry, mature protein
19970
-
calculation from amino-acid sequence
22000
-
x * 22000, SDS-PAGE
22320
-
N-terminal propeptide, calculation from amino-acid sequence
3298
-
preproenzyme, calculation from amino-acid sequence
45582
-
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination
20000
-
mature enzyme, SDS-PAGE
20000
-
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE
20000
-
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination
42000
-
SDS-PAGE, LasA is synthesized as a preproenzyme that undergoes proteolysis to remove a 22000 Da amino-terminal propeptide
42000
-
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE
42000
-
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination
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proteolytic modification
-
enzyme precursor contains an elastase cleavage motif at the N-terminus, the enzyme is probably activated by elastase to activate the elastase afterwards
proteolytic modification
-
no autoproteolysis, enzyme is synthesized as a pre-proLasA, the signal sequence of pre-proLasA is cleaved off by a signal peptidase, the remaining inactive 42 kDa proLasA is secreted to the extracellular space, where the proform is cleaved to the mature active enzyme via a 28 kDa intermediate, plus a 14 kDa fragment, by elastase and a lysine specific protease, not by alkaline proteinase
proteolytic modification
-
no autoproteolysis, the 31 amino acid residues signal sequence of pre-proLasA is cleaved off by a signal peptidase, processing of the remaining 42 kDa proLasA by incubation with the culture filtrate of Pseudomonas aeruginosa or trypsin to the active 20 kDa LasA
proteolytic modification
cleavage of proLasA precursor at position 237 of full-length gene product
proteolytic modification
-
no autoproteolysis, the 31 amino acid residues signal sequence of pre-proLasA is cleaved off by a signal peptidase, processing of the remaining 42 kDa proLasA by incubation with the culture filtrate of Pseudomonas aeruginosa or trypsin to the active 20 kDa LasA
-
proteolytic modification
-
no autoproteolysis, enzyme is synthesized as a pre-proLasA, the signal sequence of pre-proLasA is cleaved off by a signal peptidase, the remaining inactive 42 kDa proLasA is secreted to the extracellular space, where the proform is cleaved to the mature active enzyme via a 28 kDa intermediate, plus a 14 kDa fragment, by elastase and a lysine specific protease, not by alkaline proteinase
-
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additional information
-
cell lysis of Staphylococcus aureus, Micrococcus radiodurans and Gaffkia tetragena
degradation
-
two-stage enzymatic reaction for the continuous measurement of LasA protease activity using a defined substrate, supplemented with Streptomyces griseus aminopeptidase for further cleavage of the product, rate of release of the chromophore can be measured spectrophotometrically
degradation
-
two-stage enzymatic reaction for the continuous measurement of LasA protease activity using a defined substrate, supplemented with Streptomyces griseus aminopeptidase for further cleavage of the product, rate of release of the chromophore can be measured spectrophotometrically
-
medicine
-
LasA protease is effective in the treatment of experimental Staphylococcus aureus keratitis in rabbits, in both early- and late-treatment protocols the clinical scores for eyes treated with LasA protease are significantly lower than those for the eyes of corresponding controls
medicine
staphylolysin is effective in the treatment of experimental methicillin-resistant Staphylococcus aureus-induced endophthalmitis in rats, and causes no morphological adverse effects to ocular tissues. Staphylolysin may be beneficial in the treatment of Staphylococcus aureus endophthalmitis in humans
medicine
-
LasA protease is an effective therapeutic agent for Staphylococcus aureus experimental keratitis. The enzyme provides long-lasting protection against several strains of Staphylococcus aureus (MSSA, MRSA COL, MRSA 02/48406, and MRSA 02/48103) with IC50 values of 0.0018-0.005 mg/ml
medicine
presence of Pseudomonas aeruginosa alters Staphylococcus aureus susceptibility to bactericidal antibiotics in a variable, strain-dependent manner. LasA endopeptidase potentiates lysis of Staphylococcus aureus by vancomycin. Vancomycin treatment of a Staphylococcus aureus mouse burn infection is potentiated by the presence of a LasA-producing Pseudomonas aeruginosa population
medicine
-
the expression of LasA and LasB genes is decreased by Satureja khuzistanica essential oil extract. The MICs of the extract for different multidrug resistant isolates are 8-12 microg/ml
medicine
-
LasA protease is effective in the treatment of experimental Staphylococcus aureus keratitis in rabbits, in both early- and late-treatment protocols the clinical scores for eyes treated with LasA protease are significantly lower than those for the eyes of corresponding controls
-
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Carnicero, A.; Mansito, T.B.; Roldan, J.M.; Falcon, M.A.
Staphylolytic enzyme from Pseudomonas aeruginosa: characterization and immunocytochemical localization
Arch. Microbiol.
154
37-41
1990
Pseudomonas aeruginosa
brenda
Peters, J.E.; Galloway, D.R.
Purification and characterization of an active fragment of the LasA protein from Pseudomonas aeruginosa: enhancement of elastase activity
J. Bacteriol.
172
2236-2240
1990
Pseudomonas aeruginosa
brenda
Gustin, J.K.; Kessler, E.; Ohman, D.E.
A substitution at His-120 in the LasA protease of Pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion
J. Bacteriol.
178
6608-6617
1996
Pseudomonas aeruginosa, Pseudomonas aeruginosa FRD1
brenda
Kessler, E.; Safrin, M.; Abrams, W.R.; Rosenbloom, J.; Ohman, D.E.
Inhibitors and specificity of Pseudomonas aeruginosa LasA
J. Biol. Chem.
272
9884-9889
1997
Pseudomonas aeruginosa
brenda
Kessler, E.; Safrin, M.; Gustin, J.K.; Ohman, D.E.
Elastase and the LasA protease of Pseudomonas aeruginosa are secreted with their propeptides
J. Biol. Chem.
273
30225-30231
1998
Pseudomonas aeruginosa, Pseudomonas aeruginosa FRD740
brenda
Peters, J.E.; Park, S.J.; Darzins, A.; Freck, L.C.; Saulnier, J.M.; Wallach, J.M.; Galloway, D.R.
Further studies on Pseudomonas aeruginosa LasA: analysis of specificity
Mol. Microbiol.
6
1155-1162
1992
Pseudomonas aeruginosa
brenda
Kessler, E.; Safrin, M.; Blumberg, S.; Ohman, D.E.
A continuous spectrophotometric assay for Pseudomonas aeruginosa LasA protease (staphylolysin) using a two-stage enzymatic reaction
Anal. Biochem.
328
225-232
2004
Pseudomonas aeruginosa, Pseudomonas aeruginosa FRD2128(pJKGT107)
brenda
Barequet, I.S.; Ben Simon, G.J.; Safrin, M.; Ohman, D.E.; Kessler, E.
Pseudomonas aeruginosa LasA protease in treatment of experimental staphylococcal keratitis
Antimicrob. Agents Chemother.
48
1681-1687
2004
Pseudomonas aeruginosa, Pseudomonas aeruginosa FRD2128(pJKGT107)
brenda
Kessler, E.; Ohman, D.E.
Staphylolysin
Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
1001-1003
2004
Pseudomonas aeruginosa
-
brenda
Elston, C.; Wallach, J.; Saulnier, J.
New continuous and specific fluorometric assays for Pseudomonas aeruginosa elastase and LasA protease
Anal. Biochem.
368
87-94
2007
Pseudomonas aeruginosa
brenda
Grande, K.K.; Gustin, J.K.; Kessler, E.; Ohman, D.E.
Identification of critical residues in the propeptide of LasA protease of Pseudomonas aeruginosa involved in the formation of a stable mature protease
J. Bacteriol.
189
3960-3968
2007
Pseudomonas aeruginosa
brenda
Tingpej, P.; Smith, L.; Rose, B.; Zhu, H.; Conibear, T.; Al Nassafi, K.; Manos, J.; Elkins, M.; Bye, P.; Willcox, M.; Bell, S.; Wainwright, C.; Harbour, C.
Phenotypic characterization of clonal and nonclonal Pseudomonas aeruginosa strains isolated from lungs of adults with cystic fibrosis
J. Clin. Microbiol.
45
1697-1704
2007
Pseudomonas aeruginosa
brenda
Barequet, I.S.; Habot-Wilner, Z.; Mann, O.; Safrin, M.; Ohman, D.E.; Kessler, E.; Rosner, M.
Evaluation of Pseudomonas aeruginosa staphylolysin (LasA protease) in the treatment of methicillin-resistant Staphylococcus aureus endophthalmitis in a rat model
Graefes Arch. Clin. Exp. Ophthalmol.
247
913-917
2009
Pseudomonas aeruginosa (P14789), Pseudomonas aeruginosa
brenda
Spencer, J.; Murphy, L.M.; Conners, R.; Sessions, R.B.; Gamblin, S.J.
Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases
J. Mol. Biol.
396
908-923
2010
Pseudomonas aeruginosa (P14789)
brenda
Barequet, I.S.; Bourla, N.; Pessach, Y.N.; Safrin, M.; Yankovich, D.; Ohman, D.E.; Rosner, M.; Kessler, E.
Staphylolysin is an effective therapeutic agent for Staphylococcus aureus experimental keratitis
Graefes Arch. Clin. Exp. Ophthalmol.
250
223-229
2012
Pseudomonas aeruginosa
brenda
Taganna, J.C.; Quanico, J.P.; Perono, R.M.; Amor, E.C.; Rivera, W.L.
Tannin-rich fraction from Terminalia catappa inhibits quorum sensing (QS) in Chromobacterium violaceum and the QS-controlled biofilm maturation and LasA staphylolytic activity in Pseudomonas aeruginosa
J. Ethnopharmacol.
134
865-871
2011
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 10145
brenda
Islamieh, D.I.; Afshar, D.; Esmaeili, D.
Effect of Satureja khuzistanica essential oil (SKEO) extract on expression of lasA and lasB genes in Pseudomonas aeruginosa
Iran. J. Microbiol.
11
55-59
2019
Pseudomonas aeruginosa
brenda
Radlinski, L.; Rowe, S.E.; Kartchner, L.B.; Maile, R.; Cairns, B.A.; Vitko, N.P.; Gode, C.J.; Lachiewicz, A.M.; Wolfgang, M.C.; Conlon, B.P.
Pseudomonas aeruginosa exoproducts determine antibiotic efficacy against Staphylococcus aureus
PLoS Biol.
15
e2003981
2017
Pseudomonas aeruginosa (P14789), Pseudomonas aeruginosa
brenda