Literature summary for 3.4.24.B16 extracted from

Elston, C.; Wallach, J.; Saulnier, J.
New continuous and specific fluorometric assays for Pseudomonas aeruginosa elastase and LasA protease (2007), Anal. Biochem., 368, 87-94.

Search for more literature for 3.4.24.B16

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	4-(dimethylamino)azobenzene-4'-sulfonyl-Leu-Gly-Gly-Gly-Ala-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	in 5 mM Tris-HCl buffer, pH 8.5	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	strain PA01	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose Fast Flow chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-(dimethylamino)azobenzene-4'-sulfonyl chloride-Leu-Gly-Gly-Gly-Ala-5-(2-aminoethylamino)-1-naphthalenesulfonic acid + H2O	-	Pseudomonas aeruginosa	?	-	?
additional information	no hydrolysis of 4-(dimethylamino)azobenzene-4'-sulfonyl chloride-Ala-Ala-Phe-Ala-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	Pseudomonas aeruginosa	?	-	?

Synonyms

Synonyms	Comment	Organism
LasA protease	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
15.2	-	4-(dimethylamino)azobenzene-4'-sulfonyl-Leu-Gly-Gly-Gly-Ala-5-(2-aminoethylamino)-1-naphthalenesulfonic acid	in 5 mM Tris-HCl buffer, pH 8.5	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)