Literature summary for 3.4.24.B16 extracted from

Kessler, E.; Safrin, M.; Abrams, W.R.; Rosenbloom, J.; Ohman, D.E.
Inhibitors and specificity of Pseudomonas aeruginosa LasA (1997), J. Biol. Chem., 272, 9884-9889.

Search for more literature for 3.4.24.B16

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	inhibition of the staphylolytic activity	Pseudomonas aeruginosa
dithiothreitol	inhibition of the staphylolytic activity	Pseudomonas aeruginosa
EDTA	inhibition only above 20 mM	Pseudomonas aeruginosa
EGTA	inhibition only above 20 mM	Pseudomonas aeruginosa
additional information	no inhibition by 1,7-penanthroline and 4,7-phenanthroline, activity with beta-caseinis blocked by inhibitors that do not block the staphylolytic activity, no inhibition by several serine or cysteine proteinase inhibitors including diisopropyl fluorophosphate, phenylmethylsulfonylfluoride, leupeptin, and N-ethylmaleimide	Pseudomonas aeruginosa
Nalpha-p-tosyl-L-lysine chloromethyl ketone	inhibition of the staphylolytic activity	Pseudomonas aeruginosa
phosphoramidon	inhibition of the staphylolytic activity	Pseudomonas aeruginosa
Tetraethylenepentamine	inhibition of the staphylolytic activity	Pseudomonas aeruginosa
Zn2+	in excess, inhibition of staphylolytic activity	Pseudomonas aeruginosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Pseudomonas aeruginosa	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	zinc-metalloprotease	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Elastin + H2O	Pseudomonas aeruginosa	enzyme activates elastase, and the elastolytic activity of other proteases by cleaving elastin	?	-	?
additional information	Pseudomonas aeruginosa	enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells by cleaving the peptidoglycan pentaglycine interpeptides	?	-	?
protein + H2O	Pseudomonas aeruginosa	-	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
proteolytic degradation of proteins	specific for Gly-Ala peptide bonds within Gly-Gly-Ala sequences in elastin, substrates that contain no Gly-Gly peptide bond, such as beta-casein, appear to be resistant to the enzyme	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Elastin + H2O	soluble recombinant and insoluble elastin	Pseudomonas aeruginosa	?	-	?
Elastin + H2O	enzyme activates elastase, and the elastolytic activity of other proteases by cleaving elastin	Pseudomonas aeruginosa	?	-	?
additional information	enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells by cleaving the peptidoglycan pentaglycine interpeptides	Pseudomonas aeruginosa	?	-	?
protein + H2O	-	Pseudomonas aeruginosa	peptides	-	?

Synonyms

Synonyms	Comment	Organism
M23.002	Merops-ID	Pseudomonas aeruginosa
staphylolysin	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)