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Literature summary for 3.4.24.B16 extracted from
- A continuous spectrophotometric assay for Pseudomonas aeruginosa LasA protease (staphylolysin) using a two-stage enzymatic reaction (2004), Anal. Biochem., 328, 225-232.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | two-stage enzymatic reaction for the continuous measurement of LasA protease activity using a defined substrate, supplemented with Streptomyces griseus aminopeptidase for further cleavage of the product, rate of release of the chromophore can be measured spectrophotometrically | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
succinyl-Gly-Gly-Phe-4-nitroanilide | - |
Pseudomonas aeruginosa |  |
| 11.2 | - |
succinyl-Gly-Gly-Leu-4-nitroanilide | - |
Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa FRD2128(pJKGT107) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-Gly-Gly-Leu-4-nitroanilide + H2O | - |
Pseudomonas aeruginosa | Leu-4-nitroanilide + succinyl-Gly-Gly | - |
? | |
| succinyl-Gly-Gly-Leu-4-nitroanilide + H2O | - |
Pseudomonas aeruginosa FRD2128(pJKGT107) | Leu-4-nitroanilide + succinyl-Gly-Gly | - |
? | |
| succinyl-Gly-Gly-Phe-4-nitroanilide + H2O | better substrate than succinyl-Gly-Gly-Leu-4-nitroanilide | Pseudomonas aeruginosa | Phe-4 nitroanilide + succinyl-Gly-Gly | - |
? | |
| succinyl-Gly-Gly-Phe-4-nitroanilide + H2O | better substrate than succinyl-Gly-Gly-Leu-4-nitroanilide | Pseudomonas aeruginosa FRD2128(pJKGT107) | Phe-4 nitroanilide + succinyl-Gly-Gly | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LasA protease | - |
Pseudomonas aeruginosa |
| staphylolysin | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.55 | - |
succinyl-Gly-Gly-Leu-4-nitroanilide | - |
Pseudomonas aeruginosa | |
| 11.8 | - |
succinyl-Gly-Gly-Phe-4-nitroanilide | - |
Pseudomonas aeruginosa | |
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