3.4.24.16: neurolysin

This is an abbreviated version!
For detailed information about neurolysin, go to the full flat file.

Word Map on EC 3.4.24.16

3.4.24.16

bradykinin

metallopeptidase

metalloendopeptidase

dynorphins

pro-ile

neuropeptidase

pro10-tyr11

neuromedin

hemopressins

arg8-arg9

non-at2

neurotensin-degrading

molecular biology

pharmacology

medicine

Reaction

Preferential cleavage in neurotensin: Pro10-/-Tyr =

Synonyms

endopeptidase 24.16, endopeptidase 24.16B, endopeptidase 3.4.24.16, EP 24.16, ep24.16, EP24.16c, EP24.16m, MEP, Microsomal endopeptidase, mitochondrial peptidase, MOP, More, NEL, neurolisin, neurolysin, neurotensin endopeptidase, neurotensin-cleaving enzyme, Nln, oligopeptidase M, peptidase, neurotensin endo, peptidase, neurotensin endo-, SABP, soluble angiotensin II-binding protein, Soluble angiotensin-binding protein, thimet oligopeptidase II, thimet peptidase II

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.24 Metalloendopeptidases

3.4.24.16 neurolysin

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Results	in table
3	Activating Compound
26	AA Sequence
5	Application
2	CAS Registry Number
21	Cloned(Commentary)
6	Crystallization (Commentary)
77	Disease/ Diagnostics
4	Expression
17	General Information
3	General Stability
2	IC50 Value
196	Inhibitors
11	Ki Value [mM]
167	KM Value [mM]
46	Localization
24	Metals/Ions
12	Molecular Weight [Da]
60	Natural Substrates/ Products (Substrates)
202	Organism
8	PDB ID
8	pH Optimum
1	pH Range
3	Posttranslational Modification
17	Protein Variants
15	Purification (Commentary)
1	Reaction
2	Reaction Type
63	Reference
130	Source Tissue
13	Specific Activity [micromol/min/mg]
1	Storage Stability
343	Substrates and Products (Substrate)
13	Subunits
56	Synonyms
7	Temperature Optimum [°C]
207	Turnover Number [1/s]

Purification

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Purification on EC 3.4.24.16 - neurolysin

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4 entries

a two-step purification procedure based on the molecular size and isoelectric point of the photoradiolabeled binding protein is conducted. Purified samples are subjected to two-dimensional gel electrophoresis followed by mass spectrometry identification of proteins in the two-dimensional gel sections containing radioactivity. LC-MS/MS analysis reveal protein candidates, of which the most abundant are immunoprecipitated after photoradiolabeling. Immunoprecipitation studies indicate that the angiotensin binding site is the membrane-bound variant of metalloendopeptidase neurolysin

partial

recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha by glutathione affinity chromatography to homogeneity

Rattus norvegicus

683138

recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, ultrafiltration, and gel filtration to homogeneity, large scale production of rNln

Rattus norvegicus

P42676

754221

recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)

Rattus norvegicus

683672