Information on EC 3.4.24.16 - neurolysin

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
3.4.24.16
-
RECOMMENDED NAME
GeneOntology No.
neurolysin
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Preferential cleavage in neurotensin: Pro10-/-Tyr
show the reaction diagram
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
endopeptidase, peptides, endopeptidase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
endopeptidase 24.16
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-
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-
endopeptidase 24.16
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endopeptidase 24.16B
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-
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EP24.16c
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the cytosolic/membrane bound enzyme form
EP24.16m
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the mitochondrial enzyme form
MEP
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-
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Microsomal endopeptidase
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Microsomal endopeptidase
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neurolisin
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neurolysin
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neurotensin endopeptidase
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-
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oligopeptidase M
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oligopeptidase M
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peptidase, neurotensin endo
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peptidase, neurotensin endo-
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SABP
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-
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soluble angiotensin II-binding protein
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Soluble angiotensin-binding protein
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thimet oligopeptidase II
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neurolysin is most likely to be confused with thimet oligopeptidase II, but it is distinguished by its lack of activation by thiol compounds, inhibition by millimolar concentrations of Pro-Ile, cleavage of neurotensin at the Pro-Tyr bond, and different mobility in column chromatography
thimet peptidase II
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ep24.16
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ep24.16
additional information
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MOP
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-
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additional information
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neurolysin belongs to the M3 metallopeptidase family
additional information
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the enzyme belongs to the M3A family
additional information
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the enzyme belongs to the zinc-dependent peptidases of the metallopeptidase M3 family
CAS REGISTRY NUMBER
COMMENTARY
149371-24-4
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90463-53-9
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
steer
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-
Manually annotated by BRENDA team
C57BL/6 mice
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-
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
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Manually annotated by BRENDA team
male sprague-dawley rats
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-
Manually annotated by BRENDA team
male Wistar rats, strain HSdCpB: WU
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-
Manually annotated by BRENDA team
Wistar rats
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Manually annotated by BRENDA team
additional information
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
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brain membranes of mice lacking neurolysin are nearly devoid of the non-AT1, non-AT2 angiotensin binding site
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2 + H2O
(7-methoxy-coumarin-4-yl)acetyl-RPKP + YA-Nva-WMK(2,4-dinitrophenyl)-NH2
show the reaction diagram
-
substrate of matrix metalloproteinases MMP3
cleavage at the peptide bond between proline and tyrosine, withabout 1/10 efficiency in wild-type compared with the cleavage of the MMPs-2/9-specific peptide (7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2
-
?
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 + H2O
(7-methoxy-coumarin-4-yl)acetyl-RPKP + YA-Nva-WMK(2,4-dinitrophenyl)-NH2
show the reaction diagram
-
substrate of matrix metalloproteinases MMP-2/-9
cleavage at the peptide bond between proline and tyrosine
-
?
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl + H2O
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg + Ala-Lys-dinitrophenyl
show the reaction diagram
-
-
-
?
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl + H2O
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg + Ala-Lys-dinitrophenyl
show the reaction diagram
-
i.e. QF37, cleavage site: Arg6-Ala7
-
?
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl + H2O
peptide fragments
show the reaction diagram
-
i.e. QF34, cleavage sites: Leu4-Arg5, Arg5-Arg6
-
-
?
(o-aminobenzoyl)-AFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-AFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-AF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-DFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-DFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-DF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-EFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-EFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-EF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-ENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-ENKPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-ENKPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
36% cleavage at P-Y bond, 64% cleavage at Y-I bond
-
?
(o-aminobenzoyl)-ENKPRRPYIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-ENKPRRP + YIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + IQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
79% cleavage at P-Y bond, 21% cleavage at Y-I bond
-
?
(o-aminobenzoyl)-ENKPRRPYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-ENKPRRP + YQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + Q-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-ENKPR + RPYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
16% cleavage at P-Y bond, 40% cleavage at Y-Q bond, 44% cleavage at R-R
-
?
(o-aminobenzoyl)-FFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-FFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-FF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GASPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GASP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GDSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GDSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GESPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GESP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFAPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFAP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFDPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFDP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFEPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFEP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFFPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFFP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFHPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFHP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFIPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFIP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFLPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFLP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFNPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFNP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFPPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFPP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFQPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFQP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFRPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFRP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSAFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSA + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSDFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSH + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSEFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSD + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSFFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSF + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSIFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSI + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSLFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSL + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSNFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSN + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPARQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + ARQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + DRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPERQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + ERQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPERQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFAQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FAQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFDQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FEQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFFQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FFQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFLQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FQQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFR-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GF + SPFR-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
6% of the activity with (o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-GFSPFRA-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRA-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRE-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRE-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRF-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRF-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRI-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRI-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRL-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRL-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRN-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRN-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRP-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRP-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-, 20% of the activity with (o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-GFSPFRR-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRR-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRS-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRS-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRSSRIGEIKEEQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSPFRS + SRIGEIKEEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
10% of the activity with (o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSPFRS + SRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FSQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPHRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + HRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + IRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + LRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPNRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + NRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPPRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + PRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPQRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + QRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPRRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + RRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPSRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + SRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSQFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSE + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSRFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSSFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSS + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSXFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSQ + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRDQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRREQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + REQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRFQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RFQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + FQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + HQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + IQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRLQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RLQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + LQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + NQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRV-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RV-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRVQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RVQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + YQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GHSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GHSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GISPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GISP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GLSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GLSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GNSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GNSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GPSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GPSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GQSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GQSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GRSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GRSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GSSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GSSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-HFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-HFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-IFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-IFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-IF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-KPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-KPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-KPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
37% cleavage at P-Y bond, 63% cleavage at Y-I bond
-
?
(o-aminobenzoyl)-LFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-LFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-LF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-LYENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-LYENKPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-LYENKPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-LYENKPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
65% cleavage at P-Y bond, 35% cleavage at Y-I bond
-
?
(o-aminobenzoyl)-NFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-NFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-NF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-NKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-QFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-QFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-QF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-RFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-RF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-RPPGFSPFR-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RPPGF + SPFR-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
7% of the activity with (o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-RPPGFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RPPGFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
23% of the activity with (o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-RPPGFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RPPGFSPFRS + SRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
38% of the activity with (o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-SFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-SFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-SF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl + H2O
?
show the reaction diagram
-
-
-
-
?
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
?
Abz-GDSPFRQ-EDDnp + H2O
Abz-GDSP + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GESPFRQ-EDDnp + H2O
Abz-GESP + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSAFRQEDDnp + H2O
Abz-GFSAFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSEFRQEDDnp + H2O
Abz-GFSEFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSFFRQEDDnp + H2O
Abz-GFSFFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSHFRQEDDnp + H2O
Abz-GFSHFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSIFR-EDDnp + H2O
Abz-GFSIFR + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSIFRQ-EDDnp + H2O
Abz-GFSIFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSLFRQEDDnp + H2O
Abz-GFSLFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPFR-EDDnp + H2O
Abz-GFSPFR + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPFRQ-EDDnp + H2O
Abz-GFSPFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPFRQ-EDDnp + H2O
Abz-GFSPFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSQFRQEDDnp + H2O
Abz-GFSQFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSRFRQEDDnp + H2O
Abz-GFSRFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSSFRQEDDnp + H2O
Abz-GFSSFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSWFRQEDDnp + H2O
Abz-GFSWFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSYFRQEDDnp + H2O
Abz-GFSYFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GGSPFRQEDDnp + H2O
Abz-GGSP + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GPSPFRQ-EDDnp + H2O
Abz-GPSP + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-NKPRRPQEDDnp + H2O
Abz-NKPRRPQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RPPGFSPFRQEDDnp + H2O
Abz-RPPGFSPFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
acetyl-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
acetyl-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
i.e. acetyneurotensin(8-13)
-
?
Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys + H2O
?
show the reaction diagram
-
no cleavage
-
-
-
Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys + H2O
?
show the reaction diagram
-
i.e. somatostatin, cleavage sites: Phe6-Phe7, Thr10-Phe11
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
-
-
-
?
Angiotensin II + H2O
?
show the reaction diagram
-
-
-
?
Arg-Arg-Pro-Tyr-Ile-Leu + H2O
Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
-
?
Arg-Arg-Pro-Tyr-Ile-Leu + H2O
Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
i.e. neurotensin(8-13)
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
Arg-Pro-Lys-Pro-Gln + Gln-Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro-Gln-Gln + Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro
show the reaction diagram
-
-
-
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
Arg-Pro-Lys-Pro-Gln + Gln-Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro-Gln-Gln + Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro
show the reaction diagram
-
cleavage sites: Gln5-Gln6, Phe8-Gly9
-
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
Arg-Pro-Lys-Pro-Gln + Gln-Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro-Gln-Gln + Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro
show the reaction diagram
-
cleavage sites: Pro4-Gly5, Glyn5-Glyn6, and Phe8-Gly9
-
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
Arg-Pro-Lys-Pro-Gln + Gln-Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro-Gln-Gln + Phe-Phe-Gly-Leu-Met-NH2 + Arg-Pro-Lys-Pro
show the reaction diagram
-
i.e. substance P, cleavage sites: Pro4-Gln5, Gln5-Gln6, Gln6-Phe7
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
show the reaction diagram
-
-
-
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
show the reaction diagram
-
-
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
show the reaction diagram
-
i.e. bradykinin, cleavage site: Phe5-Ser6
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
show the reaction diagram
-
i.e. bradykinin, cleavage site: Phe5-Ser6
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
show the reaction diagram
-
i.e. angiotensin II
-
-
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
show the reaction diagram
-
i.e. angiotensin II
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
-
-
-
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
-
-
-
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
-
-
-
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
-
i.e. angiotensin I, cleavage site: Pro7-Phe8
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
-
i.e. angiotensin I, cleavage site: Pro7-Phe8
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
-
i.e. angiotensin I, cleavage site: Pro7-Phe8
-
?
benzoyl-Gly-Ala-Ala-Phe-4-aminobenzoate + H2O
benzoyl-Gly + Ala-Ala-Phe-4-aminobenzoate
show the reaction diagram
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
?
DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS + H2O
?
show the reaction diagram
-
-
-
-
?
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys + H2O
peptide fragments
show the reaction diagram
-
-
-
-
?
dynorphin A(1-17) + H2O
?
show the reaction diagram
-
-
-
-
?
dynorphin A1-13 + H2O
?
show the reaction diagram
-
-
-
?
gonadotrophin releasing hormone(1-9) + H2O
?
show the reaction diagram
-
-
-
-
?
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
-
-
-
?
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
-
-
-
?
LVVYPWTQRY + H2O
?
show the reaction diagram
-
cleavage sites: LVVYP-/-W-/-T-/-Q-/-RY
-
?
neuromedin N + H2O
?
show the reaction diagram
-
degradation
-
-
?
neuromedin N + H2O
?
show the reaction diagram
-
i.e. H-Lys-Ile-Pro-Tyr-Ile-Leu-OH
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
-
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
degradation
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
cleavage at the Pro-Tyr bond
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
neurolysin regulates the amount of bioactive peptide neurotensin, which functions in the modulation of central dopaminergic and cholinergic circuits, thermoregulation, intestinal motility, and blood pressure regulation. In cancer cells, neurotensin accelerates cell progression
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
cleavage of Pro-Tyr bonds
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
cleavage of the Pro10-Tyr11 bond
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
determination of specific cleavage sites, overview
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr
show the reaction diagram
-
i.e. neurotensin(1-11)
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
-
-
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
i.e. neurotensin
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
i.e. neurotensin
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
specific hydrolysis of the Pro10-Tyr11 bond
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
specific hydrolysis of the Pro10-Tyr11 bond
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
specific hydrolysis of the Pro10-Tyr11 bond
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
specific hydrolysis of the Pro10-Tyr11 bond
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
specific hydrolysis of the Pro10-Tyr11 bond
-
?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro + Tyr-Ile-Leu
show the reaction diagram
-
cleavage sites: Arg8-Arg9 and Pro10-Tyr11
-
-
?
PVNFKFLSH + H2O
?
show the reaction diagram
-
cleavage sites: PVNF-/-K-/-F-/-LSH
-
?
Pz-Pro-Leu-Gly-Pro-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
t-butyloxycarbonyl-Phe-Ala-Ala-Phe-4-aminobenzoate + H2O
t-butyloxycarbonyl-Phe + Ala-Ala-Phe-4-aminobenzoate
show the reaction diagram
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg + H2O
Tyr-Gly-Gly-Phe + Leu-Arg
show the reaction diagram
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg + H2O
?
show the reaction diagram
-
cleavage sites: Phe4-Leu5, Leu5-Arg6
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
show the reaction diagram
-
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
show the reaction diagram
-
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
show the reaction diagram
-
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
show the reaction diagram
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
show the reaction diagram
-
i.e. dynorphin(1-8)
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
?
show the reaction diagram
-
cleavage sites: Leu-5-Arg6, Arg6-Arg7
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg + H2O
peptide fragments
show the reaction diagram
-
i.e. dynorphin(1-9)
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg + H2O
Tyr-Gly-Gly-Phe-Leu-Arg-Arg + Ile-Arg
show the reaction diagram
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O
peptide fragments
show the reaction diagram
-
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O
peptide fragments
show the reaction diagram
-
i.e. dynorphin A(1-17), cleavage sites: Lys11-Leu12, Leu12-Lys13
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O
peptide fragments
show the reaction diagram
-
cleavage sites: Lys11-Leu12, Leu12-Lys13
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro + H2O
Tyr-Gly-Gly-Phe-Leu + Arg-Lys-Tyr-Pro + Tyr-Gly-Gly-Phe-Leu-Arg + Lys-Tyr-Pro
show the reaction diagram
-
i.e. beta-neoendorphin, cleavage sites: Leu5-Arg6, Arg6-Lys7
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys + H2O
Tyr-Gly-Gly-Phe-Leu-Arg-Lys + Tyr-Pro-Lys
show the reaction diagram
-
i.e. alpha-neoendorphin
-
?
Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-(NH2) + H2O
Tyr-Gly-Gly-Phe-Met + Arg-Arg-Val-(NH2)
show the reaction diagram
-
i.e. metorphinamide
-
?
Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu + H2O
Tyr-Gly-Gly-Phe + Met-Arg-Arg-Val-Gly + Arg-Pro-Glu + Tyr-Gly-Gly-Phe-Met-Arg + -Arg-Val-Gly
show the reaction diagram
-
i.e. BAM-12P, cleavage sites: Phe4-Met5, Arg6-Arg7, Gly9-Arg10
-
?
Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O
Tyr-Pro-Phe-Pro + Gly-Pro-Ile
show the reaction diagram
-
i.e. beta-casomorphin, cleavage site: Pro4-Gly5
-
?
VVYPWTQRY + H2O
?
show the reaction diagram
-
cleavage sites: VVYPW-/-T-/-Q-/-RY
-
?
[(7-methoxycoumarin-4-yl)acetyl]-APAKFFRLK(Dnp)-NH2 + H2O
[(7-methoxycoumarin-4-yl)acetyl]-APAK + FFRLK(Dnp)-NH2
show the reaction diagram
-
best substrate
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-DEVDAPK(Dnp)-NH2 + H2O
?
show the reaction diagram
-
very low activity
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-GKPILFFRLK(Dnp)DR-NH2 + H2O
[(7-methoxycoumarin-4-yl)acetyl]-GKP + ILFFRLK(Dnp)DR-NH2
show the reaction diagram
-
high activity
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-GKPILFFRLK(Dnp)DR-NH2 + H2O
[(7-methoxycoumarin-4-yl)acetyl]-GKPIL + FFRLK(Dnp)DR-NH2
show the reaction diagram
-
high activity
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-GSPAFLAK(Dnp)DR-NH2 + H2O
[(7-methoxycoumarin-4-yl)acetyl]-GSPA + FLAK(Dnp)DR-NH2
show the reaction diagram
-
low activity
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-RPKPYANvaWMK(Dnp)-NH2 + H2O
[(7-methoxycoumarin-4-yl)acetyl]-RPKP + YANvaWMK(Dnp)-NH2
show the reaction diagram
-
high activity
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-RPKPYANvaWMK(Dnp)-NH2 + H2O
[(7-methoxycoumarin-4-yl)acetyl]-RPKPY + ANvaWMK(Dnp)-NH2
show the reaction diagram
-
high activity
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-RPKPYANvaWMK(Dnp)-NH2 + H2O
?
show the reaction diagram
-
a substrate of MMP-2 and MMP-9
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-RPPGFSAFK(Dnp)-OH + H2O
?
show the reaction diagram
-
-
-
-
?
[(7-methoxycoumarin-4-yl)acetyl]-RPPGFSAFK(Dnp)-OH + H2O
[(7-methoxycoumarin-4-yl)acetyl]-RPPGFSA + FK(Dnp)-OH
show the reaction diagram
-
low activity
-
-
?
(o-aminobenzoyl)-GFSHFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSR + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSHFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSR + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
additional information
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
additional information
-
-
-
-
?
Lys-Ile-Pro-Tyr-Ile-Leu + H2O
Lys-Ile-Pro + Tyr-Ile-Leu
show the reaction diagram
-
i.e. neuromedin
-
?
additional information
?
-
-
marked specificity towards Pro-X bonds present in the interior parts of various neuropeptides and related peptides. No cleavage is observed at the first and second peptide bonds from the NH2-terimini or from the COOH-terminal extension of the peptides examined, suggesting that the enzyme requires both NH2- and COOH-terminal extensions of at least 3 residues from the scissile bond for its action. Not strictly specific for Pro-X bonds
-
-
-
additional information
?
-
-
no activity with (o-aminobenzoyl)-LGMISLMKRPPGFSPFRSSRI-NH2
-
?
additional information
?
-
-
no activity with (o-aminobenzoyl)-PRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
additional information
?
-
-
the enzyme can participate in the physiological inactivation of neurotensin
-
-
-
additional information
?
-
-
the enzyme secreted from astrocytes would act in the extracellular space, thereby restricting diffusion of released neurotensin. The neuronal membrane-associated activity would be responsible for the physiological inactivation of the peptide either in the synaptic cleft, beside the neurotensin receptors, or inside early endosomal compartments in which receptor-ligand complexes would have been internalized
-
-
-
additional information
?
-
-
possible implication of the enzyme in the specific degradation of neurotensin and other peptide neurotransmitters in the synaptic cleft
-
-
-
additional information
?
-
-
EC 3.4.24.16 and EC 3.4.24.15 are responsible for the inactivation of neurotensin, somatostatin, and other neuropeptides in the brain
-
-
-
additional information
?
-
-
the enzyme contributes to the catabolism of neurotensin in the dog intestine
-
-
-
additional information
?
-
-
the enzyme is likely involved in the physiological termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract
-
-
-
additional information
?
-
-
the enzyme cleaves several bioactive peptides at sites similar or different from thimet oligopeptidase, EC 3.4.24.15
-
-
-
additional information
?
-
-
the enzyme is active in inactivation of neuropeptides, biological relevance, overview
-
-
-
additional information
?
-
-
the enzyme is involved in angiogenesis and tumor growth, overview
-
-
-
additional information
?
-
-
the enzyme is involved in the inactivation of numerous neuropeptides
-
-
-
additional information
?
-
-
activity of the recombinant enzyme with fluorescence-quenching peptides, cleavage site and amino acid preference, overview
-
-
-
additional information
?
-
-
cleavage sites of wild-type and mutant enzymes, overview
-
-
-
additional information
?
-
-
residues His425, Ala426, Cys428, Pro193, Gln482, Asp410, Thr495, Leu397, and Thr471 are involved in substrate recognition, as well as the tyrosine-rich loop 604GGYDGQYYGY613, overview, poor activity with the MMP-3 substrate [(7-methoxycoumarin-4-yl)acetyl]-RPKPVENvaWRK(Dnp[2,4-dinitrophenyl])-NH2
-
-
-
additional information
?
-
-
residues R470, R491, N496, and T499 determine the substrate specificity of thimet oligopeptidase different from closely related thimet oligopeptidase, EC 3.4.24.15
-
-
-
additional information
?
-
-
substrate and cleavage site specificity, overview, no activity with GFPPFRQ
-
-
-
additional information
?
-
-
the catalytic site, with the active site sequence motif HEXXH, is located in a deep channel that provides access only to short peptides, but NEL shows a broad substrate specificity, in part due to the presence of a flexible loop lined with the enzyme binding site of the peptidase
-
-
-
additional information
?
-
-
membrane-bound variant of neurolysin functions as the non-angiotensin Type 1 (non-AT1), non-AT2 Angiotensin binding site
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
neuromedin N + H2O
?
show the reaction diagram
-
degradation
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
degradation
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
neurolysin regulates the amount of bioactive peptide neurotensin, which functions in the modulation of central dopaminergic and cholinergic circuits, thermoregulation, intestinal motility, and blood pressure regulation. In cancer cells, neurotensin accelerates cell progression
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme can participate in the physiological inactivation of neurotensin
-
-
-
additional information
?
-
-
the enzyme secreted from astrocytes would act in the extracellular space, thereby restricting diffusion of released neurotensin. The neuronal membrane-associated activity would be responsible for the physiological inactivation of the peptide either in the synaptic cleft, beside the neurotensin receptors, or inside early endosomal compartments in which receptor-ligand complexes would have been internalized
-
-
-
additional information
?
-
-
possible implication of the enzyme in the specific degradation of neurotensin and other peptide neurotransmitters in the synaptic cleft
-
-
-
additional information
?
-
-
EC 3.4.24.16 and EC 3.4.24.15 are responsible for the inactivation of neurotensin, somatostatin, and other neuropeptides in the brain
-
-
-
additional information
?
-
-
the enzyme contributes to the catabolism of neurotensin in the dog intestine
-
-
-
additional information
?
-
-
the enzyme is likely involved in the physiological termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract
-
-
-
additional information
?
-
-
the enzyme cleaves several bioactive peptides at sites similar or different from thimet oligopeptidase, EC 3.4.24.15
-
-
-
additional information
?
-
-
the enzyme is active in inactivation of neuropeptides, biological relevance, overview
-
-
-
additional information
?
-
-
the enzyme is involved in angiogenesis and tumor growth, overview
-
-
-
additional information
?
-
-
the enzyme is involved in the inactivation of numerous neuropeptides
-
-
-
additional information
?
-
-
membrane-bound variant of neurolysin functions as the non-angiotensin Type 1 (non-AT1), non-AT2 Angiotensin binding site
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Na2SO4
-
strong activation
NaCl
-
strong activation
NaI
-
modest activation
NH4Cl
-
modest activation
Zinc
-
zinc metalloprotease
Zn2+
-
0.001 mM, stimulates
Zn2+
-
dependent on, neurolysin contains a His-Glu-Xaa-Xaa-His zinc binding motif in its active domain that traps a zinc molecule using two histidine residues
Zn2+
-
a zinc-dependent metallopeptidase
KCl
-
strong activation
additional information
-
metallopeptidase
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2R)-2-[(4-biphenylylsulfonyl)amino]-3-phenylpropionic acid
-
-
(2R)-[(4-biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide
-
-
1,10-phenanthroline
-
1 mM, 95% inhibition
1,10-phenanthroline
-
-
1,10-phenanthroline
-
-
3-(4-phenoxyphenylsulfonyl)-propylthiirane
-
-
acetyl-neurotensin(8-13)
-
degradation of tritiated neurotensin
acetyl-neurotensin(8-13)
-
inhibits degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
acetyl-neurotensin(8-13)aminde
-
degradation of tritiated neurotensin
-
agaricoglyceride A
-
main active principle of the crude extract of Agaricus macrosporus shows strong activity against neurolysin
agaricoglyceride B
-
agaricoglyceride C, agaricoglyceride D, known cometabolites agaricic ester show no activity against neurolysin up to 0.01 mM
alpha-melanotrophin-stimulating hormone
-
weak
-
angiotensin I
-
-
angiotensin I
-
inhibits degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
angiotensin I
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
angiotensin II
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
angiotensin II
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
Arg-Pro
-
-
bombesin
-
weak
bradykinin
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
bradykinin
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
carboxy-2-phenylethyl-Ala-Ala-Phe-4-aminobenzoate
-
-
-
carboxyphenylethyl-Ala-Ala-Phe-4-aminobenzoate
-
0.001 mM, 30% inhibition
-
carboxyphenylethyl-Ala-Ala-Phe-4-aminobenzoate
-
-
-
carboxyphenylethyl-Ala-Ala-Tyr-4-aminobenzoate
-
-
-
dithiothreitol
-
-
dithiothreitol
-
-
dynorphin A(1-13)
-
-
dynorphin A1-13
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
dynorphin(1-8)
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
dynorphin(1-9)
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
EDTA
-
1 mM, 51% inhibition
EDTA
-
reactivation by Zn2+
EDTA
-
complete inhibition at 4 mg/ml
H-Cys1-Thr-Thr-His-Trp-Gly-Phe-Thr-Leu-Cys10-OH
-
-
HONHCO-CH2-CH(CH2-C6H5)CO-Ile-Ala
-
potent and selective inhibitor of neurolysin
iodoacetamide
-
10 mM, 22% inactivation
iodoacetamide
-
10 mM, 15% inactivation
JA-2
-
i.e. N-[1-(R,S)-carboxy-3-phenylpropyl]Ala-Aib-Tyrp-aminobenzoate, the aromatic ring of Tyr605 was an important anchor for its interaction with wild-type TOP
JA-2
-
almost complete inhibition at 0.003 mM
kinetensin
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
Leupeptin
-
11% inhibition
Leupeptin
-
1 mM, 22% inhibition
LVVYPWTQRY
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
molecular inhibitors of Gbetagama-dimer function
-
strain-dependent increases in EP24.16 activity are substantially attenuated by betaARK.ct by 86.4%
-
N-(1(R,S)-carboxy-3-phenylpropyl)-Ala-Ala-Tyr-p-aminobenzoate
-
inhibits EP24.16 resulting in a 1000fold increase in type-2 BK receptor sensitivity to bradykinin as measured by inositol phosphate accumulation
N-(phenylethylphosphonyl)-Gly-L-Pro-L-aminohexanoic acid
-
-
N-(phenylethylphosphonyl)-Gly-Pro-L-norleucine
-
-
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-4-aminobenzoate
-
-
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-4-aminobenzoate
-
-
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-4-aminobenzoate
-
-
Neuromedin N
-
-
Neuromedin N
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
neurotensin
-
inhibits degradation of tritiated neurotensin
neurotensin
-
inhibits degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
neurotensin(8-13)
-
degradation of tritiated neurotensin
neurotensin(9-13)
-
the shortest neurotensin partial sequence that is able to fully inhibit neurotensin degradation
neurotensin(9-13)
-
-
NF023
-
attenuates the strain-dependent elevation of endopeptidase activity by 82.2%
o-phenanthroline
-
complete inhibition at 4 mM
o-phenanthroline
-
strong inhibition
phosphodiepryl 03
-
-
Pro-DL-Phe-Psi(PO2CH2)-Gly-Pro-NH2
-
-
Pro-Ile
-
most potent specific inhibitor
Pro-Ile
-
selective inhibitor of neurolysin, inhibition by milimolar concentrations
Pro-Ile
-
causes a 100fold leftward shift in the concentration response curve for bradykinin-induced inositol phosphate generation, moreover leads to a potentiation of bradykinin-induced reduction in protein kinase B activity
Pro-Ile
-
48% inhibition at 1 mM
Pro-Phe-PSI(PO2CH2)Gly-Pro
-
potent and selective inhibitor of neurolysin
Pro-Phe-PSI(PO2CH2)Leu-Pro-NH2
-
potent and selective inhibitor of neurolysin
Pro-Xaa dipeptides
-
most potent inhibitors, Xaa-Pro dipeptides are ineffective
PTX
-
completely attenuates the cyclic strain-dependent increase in endopeptidase activity
-
PVNFKFLSH
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
S-nitroso-N-acetylpenicillamine
-
inhibition is prevented by DTT
Somatostatin
-
weak
Substance P
-
weak
Substance P
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
VVYPWTQRY
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
Xenopsin
-
degradation of 3-carboxy-7-methoxycoumaryl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
[acetylPhe12]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[Ala12]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[Ala13]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[D-Arg8]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[D-Arg9]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[D-Leu11]neurotensin
-
degradation of tritiated neurotensin
-
[D-Phe11]neurotensin
-
degradation of tritiated neurotensin
-
[D-Trp11]neurotensin
-
degradation of tritiated neurotensin
-
[D-Tyr11]neurotensin
-
degradation of tritiated neurotensin
-
[D-Tyr3]neurotensin
-
degradation of tritiated neurotensin
-
[Dopa11]neurotensin
-
degradation of tritiated neurotensin
-
[His11]neurotensin
-
degradation of tritiated neurotensin
-
[naphthylalanyl11]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[Phe11]neurotensin
-
degradation of tritiated neurotensin
-
[Ser11]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[Thr11]neurotensin
-
degradation of tritiated neurotensin
-
[Trp11]neurotensin
-
degradation of tritiated neurotensin
-
[Tyr-ethyl ester11]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
[Tyr13]neurotensin(8-13)
-
degradation of tritiated neurotensin
-
molecular inhibitors of Gialpha-subunit function
-
force-dependent increase in cellular EP24.16 activity is strongly attenuated by Gialpha1-G202T by 69.6% and Gialpha2-G203T by 101.7%, whereas Gialpha2-G202T has no significant effect
-
additional information
additional information
-
putative intracellular peptides as competetive inhibitors of (o-aminobenzoyl)-GFSHFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) hydrolysis by EP24.16
-
additional information
-
inhibitor of neurolysin potentiates the neurotensin-induced anti-nociception
-
additional information
-
no inhibition by bestatin, captopril, leupeptin, and E64, poor inhibition by PMSF and Z-Pro-prolinal
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
thiols
-
activate
additional information
-
not activated by thiol compounds
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
6
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2
-
mutant Y610L, 37C
14
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2
-
wild-type, 37C
7.7
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2
-
wild-type, 37C
19
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2
-
mutant Y610L, 37C
0.002
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
-
-
0.0022
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
-
-
0.0043
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl
-
-
0.0014
-
(o-aminobenzoyl)-AFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00115
-
(o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0023
-
(o-aminobenzoyl)-DFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00064
-
(o-aminobenzoyl)-EFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0058
-
(o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00083
-
(o-aminobenzoyl)-FFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0023
-
(o-aminobenzoyl)-GASPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0684
-
(o-aminobenzoyl)-GDSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0318
-
(o-aminobenzoyl)-GESPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00087
-
(o-aminobenzoyl)-GFAPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0003
-
(o-aminobenzoyl)-GFDPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0031
-
(o-aminobenzoyl)-GFEPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0011
-
(o-aminobenzoyl)-GFFPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00033
-
(o-aminobenzoyl)-GFHPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00018
-
(o-aminobenzoyl)-GFIPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00019
-
(o-aminobenzoyl)-GFLPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00045
-
(o-aminobenzoyl)-GFNPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0037
-
(o-aminobenzoyl)-GFPPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.002
-
(o-aminobenzoyl)-GFQPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00039
-
(o-aminobenzoyl)-GFRPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0011
-
(o-aminobenzoyl)-GFSAFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0018
-
(o-aminobenzoyl)-GFSDFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.003
-
(o-aminobenzoyl)-GFSEFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0015
-
(o-aminobenzoyl)-GFSFFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0013
-
(o-aminobenzoyl)-GFSHFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00085
-
(o-aminobenzoyl)-GFSIFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0018
-
(o-aminobenzoyl)-GFSLFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0013
-
(o-aminobenzoyl)-GFSNFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00012
-
(o-aminobenzoyl)-GFSPARQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0032
-
(o-aminobenzoyl)-GFSPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00022
-
(o-aminobenzoyl)-GFSPERQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00095
-
(o-aminobenzoyl)-GFSPFAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00023
-
(o-aminobenzoyl)-GFSPFDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0011
-
(o-aminobenzoyl)-GFSPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.002
-
(o-aminobenzoyl)-GFSPFFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0024
-
(o-aminobenzoyl)-GFSPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00042
-
(o-aminobenzoyl)-GFSPFIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0056
-
(o-aminobenzoyl)-GFSPFLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0033
-
(o-aminobenzoyl)-GFSPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00055
-
(o-aminobenzoyl)-GFSPFPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0061
-
(o-aminobenzoyl)-GFSPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0011
-
(o-aminobenzoyl)-GFSPFRA-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00018
-
(o-aminobenzoyl)-GFSPFRE-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00022
-
(o-aminobenzoyl)-GFSPFRF-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00044
-
(o-aminobenzoyl)-GFSPFRI-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0014
-
(o-aminobenzoyl)-GFSPFRL-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00015
-
(o-aminobenzoyl)-GFSPFRN-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0017
-
(o-aminobenzoyl)-GFSPFRP-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00058
-
(o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00021
-
(o-aminobenzoyl)-GFSPFRR-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00065
-
(o-aminobenzoyl)-GFSPFRS-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0011
-
(o-aminobenzoyl)-GFSPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00021
-
(o-aminobenzoyl)-GFSPHRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00091
-
(o-aminobenzoyl)-GFSPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0024
-
(o-aminobenzoyl)-GFSPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0002
-
(o-aminobenzoyl)-GFSPNRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0011
-
(o-aminobenzoyl)-GFSPPRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0013
-
(o-aminobenzoyl)-GFSPQRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00003
-
(o-aminobenzoyl)-GFSPRRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00078
-
(o-aminobenzoyl)-GFSPSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0024
-
(o-aminobenzoyl)-GFSQFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00058
-
(o-aminobenzoyl)-GFSRFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0111
-
(o-aminobenzoyl)-GFSSFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0016
-
(o-aminobenzoyl)-GFSXFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00047
-
(o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0005
-
(o-aminobenzoyl)-GGFLRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0033
-
(o-aminobenzoyl)-GGFLRRDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0019
-
(o-aminobenzoyl)-GGFLRREQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0007
-
(o-aminobenzoyl)-GGFLRRFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0017
-
(o-aminobenzoyl)-GGFLRRHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0018
-
(o-aminobenzoyl)-GGFLRRIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0007
-
(o-aminobenzoyl)-GGFLRRLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0015
-
(o-aminobenzoyl)-GGFLRRNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0012
-
(o-aminobenzoyl)-GGFLRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0023
-
(o-aminobenzoyl)-GGFLRRRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0022
-
(o-aminobenzoyl)-GGFLRRV-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0021
-
(o-aminobenzoyl)-GGFLRRVQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0006
-
(o-aminobenzoyl)-GGFLRRYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0025
-
(o-aminobenzoyl)-GHSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0047
-
(o-aminobenzoyl)-GISPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0059
-
(o-aminobenzoyl)-GLSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0075
-
(o-aminobenzoyl)-GNSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0063
-
(o-aminobenzoyl)-GPSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0068
-
(o-aminobenzoyl)-GQSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0013
-
(o-aminobenzoyl)-GRSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0058
-
(o-aminobenzoyl)-GSSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00033
-
(o-aminobenzoyl)-HFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0012
-
(o-aminobenzoyl)-IFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0033
-
(o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0017
-
(o-aminobenzoyl)-LFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00044
-
(o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00103
-
(o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0013
-
(o-aminobenzoyl)-NFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00138
-
(o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00056
-
(o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00082
-
(o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00025
-
(o-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00119
-
(o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00033
-
(o-aminobenzoyl)-QFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00032
-
(o-aminobenzoyl)-RFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00048
-
(o-aminobenzoyl)-SFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0006
-
2-aminobenzoyl-GFSPFRQ-(N-2,4-dinitrophenyl)ethylenediamine
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0007
-
2-aminobenzoyl-GFSPFRQ-(N-2,4-dinitrophenyl)ethylenediamine
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0031
-
2-aminobenzoyl-GFSPFRQ-(N-2,4-dinitrophenyl)ethylenediamine
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0498
-
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl
-
abvove
0.0498
-
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl
-
-
0.0009
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0011
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0058
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0078
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0024
-
Abz-GFSEFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.005
-
Abz-GFSEFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0053
-
Abz-GFSEFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0015
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0025
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0098
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0101
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0018
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0059
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0104
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0106
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0008
-
Abz-GFSIFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A; pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0048
-
Abz-GFSIFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0018
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0019
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0048
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0061
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0016
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0054
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0096
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.021
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0008
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0013
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0019
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0026
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0083
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0056
-
Abz-GFSSFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0074
-
Abz-GFSSFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A; pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0111
-
Abz-GFSSFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0008
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.0022
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0029
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.006
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0012
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.0038
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.0065
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.0096
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.002
-
neurotensin
-
recombinant wild-type enzyme, pH 7.5, 37C
0.0026
-
neurotensin
-
-
0.00295
-
neurotensin
-
recombinant mutant R470E/T499R, pH 7.5, 37C
0.007
-
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
-
0.085
-
Tyr-Gly-Gly-Phe-Leu-Arg
-
-
0.041
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg
-
-
0.007
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
-
0.013
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg
-
-
0.05
-
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
-
additional information
-
additional information
-
Km-values for the reaction with angiotensin I analogs
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.76
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2
-
wild-type, 37C
1.1
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2
-
mutant Y610L, 37C
1.9
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2
-
mutant Y610L, 37C
2.1
-
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2
-
wild-type, 37C
11.9
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
-
-
2.1
-
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl
-
-
0.1
-
(o-aminobenzoyl)-AFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.27
-
(o-aminobenzoyl)-AFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.00497
-
(o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.02
-
(o-aminobenzoyl)-DFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.12
-
(o-aminobenzoyl)-DFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.01
-
(o-aminobenzoyl)-EFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.08
-
(o-aminobenzoyl)-EFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.35
-
(o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.03
-
(o-aminobenzoyl)-FFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.23
-
(o-aminobenzoyl)-FFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.3
-
(o-aminobenzoyl)-GASPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.34
-
(o-aminobenzoyl)-GDSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.84
-
(o-aminobenzoyl)-GESPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GESPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.8
-
(o-aminobenzoyl)-GFAPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.2
-
(o-aminobenzoyl)-GFDPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.07
-
(o-aminobenzoyl)-GFEPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.28
-
(o-aminobenzoyl)-GFFPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GFFPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.52
-
(o-aminobenzoyl)-GFHPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GFHPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.27
-
(o-aminobenzoyl)-GFIPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.24
-
(o-aminobenzoyl)-GFLPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.64
-
(o-aminobenzoyl)-GFNPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GFNPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.62
-
(o-aminobenzoyl)-GFPPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GFPPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.39
-
(o-aminobenzoyl)-GFQPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.97
-
(o-aminobenzoyl)-GFRPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GFRPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
2.6
-
(o-aminobenzoyl)-GFSAFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
2.5
-
(o-aminobenzoyl)-GFSDFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.19
-
(o-aminobenzoyl)-GFSEFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
3.4
-
(o-aminobenzoyl)-GFSFFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
4.8
-
(o-aminobenzoyl)-GFSHFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.22
-
(o-aminobenzoyl)-GFSIFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
3.5
-
(o-aminobenzoyl)-GFSLFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
8.8
-
(o-aminobenzoyl)-GFSNFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.4
-
(o-aminobenzoyl)-GFSPARQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
0.03
-
(o-aminobenzoyl)-GFSPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.24
-
(o-aminobenzoyl)-GFSPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
0.14
-
(o-aminobenzoyl)-GFSPERQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.65
-
(o-aminobenzoyl)-GFSPERQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
1.3
-
(o-aminobenzoyl)-GFSPFAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
1.4
-
(o-aminobenzoyl)-GFSPFAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.8
-
(o-aminobenzoyl)-GFSPFDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.29
-
(o-aminobenzoyl)-GFSPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.6
-
(o-aminobenzoyl)-GFSPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.4
-
(o-aminobenzoyl)-GFSPFFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.4
-
(o-aminobenzoyl)-GFSPFFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.88
-
(o-aminobenzoyl)-GFSPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.4
-
(o-aminobenzoyl)-GFSPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
6.08
-
(o-aminobenzoyl)-GFSPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.61
-
(o-aminobenzoyl)-GFSPFIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
6.08
-
(o-aminobenzoyl)-GFSPFIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
5.9
-
(o-aminobenzoyl)-GFSPFLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.64
-
(o-aminobenzoyl)-GFSPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.1
-
(o-aminobenzoyl)-GFSPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
6.08
-
(o-aminobenzoyl)-GFSPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.4
-
(o-aminobenzoyl)-GFSPFPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.56
-
(o-aminobenzoyl)-GFSPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.73
-
(o-aminobenzoyl)-GFSPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
6.08
-
(o-aminobenzoyl)-GFSPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond; pH 7.4, 37C, cleavage of P-F bond
3
-
(o-aminobenzoyl)-GFSPFRA-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.1
-
(o-aminobenzoyl)-GFSPFRE-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.8
-
(o-aminobenzoyl)-GFSPFRF-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.9
-
(o-aminobenzoyl)-GFSPFRI-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
7.4
-
(o-aminobenzoyl)-GFSPFRL-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.9
-
(o-aminobenzoyl)-GFSPFRN-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.83
-
(o-aminobenzoyl)-GFSPFRP-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GFSPFRP-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.31
-
(o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C; pH 7.4, 37C, cleavage of P-F bond; pH 7.4, 37C, cleavage of P-X bond
0.45
-
(o-aminobenzoyl)-GFSPFRR-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
2.1
-
(o-aminobenzoyl)-GFSPFRS-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.1
-
(o-aminobenzoyl)-GFSPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
1.3
-
(o-aminobenzoyl)-GFSPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.19
-
(o-aminobenzoyl)-GFSPHRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
0.53
-
(o-aminobenzoyl)-GFSPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.65
-
(o-aminobenzoyl)-GFSPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
6.08
-
(o-aminobenzoyl)-GFSPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.55
-
(o-aminobenzoyl)-GFSPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
1.3
-
(o-aminobenzoyl)-GFSPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.62
-
(o-aminobenzoyl)-GFSPNRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
6.08
-
(o-aminobenzoyl)-GFSPNRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
0.15
-
(o-aminobenzoyl)-GFSPPRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
2.6
-
(o-aminobenzoyl)-GFSPQRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
0.42
-
(o-aminobenzoyl)-GFSPRRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
1.5
-
(o-aminobenzoyl)-GFSPSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-X bond
0.67
-
(o-aminobenzoyl)-GFSQFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.08
-
(o-aminobenzoyl)-GFSQFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.31
-
(o-aminobenzoyl)-GFSRFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
7.5
-
(o-aminobenzoyl)-GFSSFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
3.6
-
(o-aminobenzoyl)-GFSXFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.77
-
(o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
2.94
-
(o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
3.2
-
(o-aminobenzoyl)-GGFLRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
1
-
(o-aminobenzoyl)-GGFLRRDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
1.1
-
(o-aminobenzoyl)-GGFLRREQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
0.2
-
(o-aminobenzoyl)-GGFLRRFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
0.3
-
(o-aminobenzoyl)-GGFLRRFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-F bond
0.6
-
(o-aminobenzoyl)-GGFLRRHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-H bond
1.6
-
(o-aminobenzoyl)-GGFLRRHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
0.1
-
(o-aminobenzoyl)-GGFLRRIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-I bond
0.7
-
(o-aminobenzoyl)-GGFLRRIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
0.2
-
(o-aminobenzoyl)-GGFLRRLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
0.5
-
(o-aminobenzoyl)-GGFLRRLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-L bond
0.3
-
(o-aminobenzoyl)-GGFLRRNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-N bond
0.6
-
(o-aminobenzoyl)-GGFLRRNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
1.8
-
(o-aminobenzoyl)-GGFLRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
3.3
-
(o-aminobenzoyl)-GGFLRRRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
2
-
(o-aminobenzoyl)-GGFLRRV-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
1.4
-
(o-aminobenzoyl)-GGFLRRVQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
0.2
-
(o-aminobenzoyl)-GGFLRRYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-Y bond
0.3
-
(o-aminobenzoyl)-GGFLRRYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C, cleavage of the R-R bond
0.95
-
(o-aminobenzoyl)-GHSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
2.6
-
(o-aminobenzoyl)-GISPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
6.2
-
(o-aminobenzoyl)-GLSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.3
-
(o-aminobenzoyl)-GNSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
2.2
-
(o-aminobenzoyl)-GPSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.9
-
(o-aminobenzoyl)-GQSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
5.4
-
(o-aminobenzoyl)-GRSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
2
-
(o-aminobenzoyl)-GSSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
1.2
-
(o-aminobenzoyl)-HFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.05
-
(o-aminobenzoyl)-IFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.45
-
(o-aminobenzoyl)-IFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
2.63
-
(o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
3
6
(o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.13
-
(o-aminobenzoyl)-LFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.35
-
(o-aminobenzoyl)-LFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
3.8
-
(o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00697
-
(o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.16
-
(o-aminobenzoyl)-NFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
1
-
(o-aminobenzoyl)-NFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.00576
-
(o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00123
-
(o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.014
-
(o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.00124
-
(o-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
7
-
(o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.12
-
(o-aminobenzoyl)-QFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.39
-
(o-aminobenzoyl)-QFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.02
-
(o-aminobenzoyl)-RFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.27
-
(o-aminobenzoyl)-RFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.12
-
(o-aminobenzoyl)-SFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of F-S bond
0.77
-
(o-aminobenzoyl)-SFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
6.08
-
(o-aminobenzoyl)-SFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C, cleavage of P-F bond
0.006
-
2-aminobenzoyl-GFSPFRQ-(N-2,4-dinitrophenyl)ethylenediamine
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.1
-
2-aminobenzoyl-GFSPFRQ-(N-2,4-dinitrophenyl)ethylenediamine
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.3
-
2-aminobenzoyl-GFSPFRQ-(N-2,4-dinitrophenyl)ethylenediamine
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.2
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.5
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.8
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
2.6
-
Abz-GFSAFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.1
-
Abz-GFSEFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.7
-
Abz-GFSEFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
1.2
-
Abz-GFSEFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.09
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
1.1
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
3.4
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
4.9
-
Abz-GFSFFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.05
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.2
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
2.5
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
6.3
-
Abz-GFSHFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.01
-
Abz-GFSIFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.04
-
Abz-GFSIFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.2
-
Abz-GFSIFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.1
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.7
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
2.4
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
3.5
-
Abz-GFSLFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.6
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
0.7
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
3.6
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
5.9
-
Abz-GFSQFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.4
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.7
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.9
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
3.3
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
4.8
-
Abz-GFSRFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.06
-
Abz-GFSSFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
0.2
-
Abz-GFSSFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
2.4
-
Abz-GFSSFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
7.5
-
Abz-GFSSFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
0.3
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
1
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
1.5
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
2.4
-
Abz-GFSWFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
0.3
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606A
1
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged wild-type enzyme
1.3
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant Y606F
2.8
-
neurotensin
-
recombinant mutant R470E/T499R, pH 7.5, 37C
5
-
neurotensin
-
recombinant wild-type enzyme, pH 7.5, 37C
0.827
-
Tyr-Gly-Gly-Phe-Leu-Arg
-
-
1.94
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg
-
-
2.52
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
-
1.5
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg
-
-
4.1
-
Abz-GFSyFRQEDDnp
-
pH 7.4, 37C, recombinant GST-tagged mutant G608A
additional information
-
additional information
-
turnover-numbers for the reaction with angiotensin I analogs
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00535
-
angiotensin I
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
0.00795
-
angiotensin II
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
0.00811
-
bradykinin
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
0.000022
-
dynorphin A1-13
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
0.00343
-
PVNFKFLSH
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
0.00704
-
VVYPWTQRY
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
0.00201
-
LVVYPWTQRY
-
inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
additional information
-
additional information
additional information
-
peptide phosphorylation largely changes the kinetics of interaction and degredation by EP24.16
-
additional information
-
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0088
-
-
purified recombinant enzyme, substrate peptide RPPGFSPFRQ
0.0091
-
-
-
0.014
-
-
-
0.0198
-
-
-
0.0226
-
-
-
0.104
-
-
-
0.298
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
quantitative determination of cleavage activity of neurolysin toward MMP-specific fluorescence-quenching peptides
additional information
-
-
-
additional information
-
-
substrate specificity, and cell proliferation assay, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-
assay at
7.4
-
-
assay at
7.5
7.6
-
-
7.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
pH-dependence of wild-type and mutant enzymes, overview
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
contain only soluble enzyme
Manually annotated by BRENDA team
-
melanoma cell line
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
melanoma cell line
-
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
colon cancer cell line
Manually annotated by BRENDA team
-
longitudinal smooth muscle membranes and circular smooth muscle membranes
Manually annotated by BRENDA team
-
neuroblastoma cells
Manually annotated by BRENDA team
-
contains both soluble and membrane-associated enzyme
Manually annotated by BRENDA team
-
primary culture
Manually annotated by BRENDA team
-
ileum, longitudinal and circular
Manually annotated by BRENDA team
additional information
-
no activity in the duodenum
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
shorter form of neurolysin, lacking the cleavable mitochondrial targeting sequence, remains in the cytosol
Manually annotated by BRENDA team
-
plasma membrane
Manually annotated by BRENDA team
-
the membrane-associated enzyme form increases during neuronal differentation and appears to be responsible for the overall augmentation of endopeptidase activity observed during neuronal maturation
Manually annotated by BRENDA team
-
EP24.16c is the cytosolic/membrane-bound enzyme form
Manually annotated by BRENDA team
-
the transfectants exhibit a membrane-associated form of endopeptidase-24.16, the catalytic site of which clearly faces the extracellular domain
Manually annotated by BRENDA team
-
the enzyme is not attached by a glycosyl-phosphatidylinositol anchor in the emembrane, the enzyme can not be released from the membrane upon trypsinolysis
Manually annotated by BRENDA team
-
synaptic and fundus membranes in brain
Manually annotated by BRENDA team
-
synaptic and smooth muscle cell membranes in brain
Manually annotated by BRENDA team
-
intermembrane space
Manually annotated by BRENDA team
-
EP24.16m is the mitochondrial enzyme form
Manually annotated by BRENDA team
-
longer form of neurolysin that contains a cleavable mitochondrial targeting sequence at the N-terminus
Manually annotated by BRENDA team
-
the enzyme secreted from astrocytes would act in the extracellular space, thereby restricting diffusion of released neurotensin
-
Manually annotated by BRENDA team
-
synaptosomal fraction
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55000
63000
-
gel filtration
66000
70000
-
gel filtration
70000
75000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 75000, SDS-PAGE
?
-
x * 80202, calculation from nucleotide sequence
monomer
-
1 * 75000, SDS-PAGE
monomer
-
1 * 70000, SDS-PAGE
monomer
-
1 * 70000, SDS-PAGE
additional information
-
Gly608 of NEL contributes to the flexibility of the loops formed by residues 600-612, i.e. GHLAGGYDGQYYG, in NEL, overview
additional information
-
structural comparison between neurolysin and MMP-9, overview
additional information
-
three-dimensional structure, comparison to EC 3.4.24.15
additional information
-
structure analysis and comparisons, modelling, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
no modification
-
no glycoprotein
no modification
-
the enzyme contains no glycosyl-phosphatidylinositol anchor
side-chain modification
-
glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystal structure analysis and comparisons, overview
-
purified recombinant mutant R470E/T499R, hanging drop vapour diffusion method, 4C, 0.001 ml of 10 mg/ml protein is mixed with 0.001 ml of well solution containing 100 mM sodium cacodylate, pH 6.5, 100 mM magnesium acetate, 2 mM 2-mercaptoethanol, and 12-14% w/v polyethylene glycol 6000, cryoprotection by 25% glycerol, X-ray diffraction structure determination and analysis at 2.2 A resolution
-
recombinant enzyme from Escherichia coli, hanging-drop vapor-diffusion method
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0.001 mM Zn2+ stabilizes
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
-
a two-step purification procedure based on the molecular size and isoelectric point of the photoradiolabeled binding protein is conducted. Purified samples are subjected to two-dimensional gel electrophoresis followed by mass spectrometry identification of proteins in the two-dimensional gel sections containing radioactivity. LC-MS/MS analysis reveal protein candidates, of which the most abundant are immunoprecipitated after photoradiolabeling. Immunoprecipitation studies indicate that the angiotensin binding site is the membrane-bound variant of metalloendopeptidase neurolysin
-
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha by glutathione affinity chromatography to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
enzyme cloning from isolated ciliary body, expression analysis
-
expressed in HEK293 cells
-
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
enzyme cloning from isolated ciliary body and liver, expression analysis
-
exhibits 60.2% homology with EC 3.4.24.15
-
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
-
expression of neurolysin gene fron brain ligated to the C-terminal half of the alpha-agglutinin gene with a FLAG tag sequence in Saccharomyces cerevisiae at the cell surface, construction of a molecular displaying plasmid
-
expression of the FLAG-tagged brain enzyme in Saccharomyces cerevisiae strain BJ2168 on the cell surface, subcloning in Escherichia coli strain DH5alpha
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
overexpressed in human kidney cells, HK293 cells. The transfectants exhibit a membrane-associated form of endopeptidase-24.16, the catalytic site of which clearly faces the extracellular domain. Transfected cells are unable to secrete the enzyme
-
overexpression of enzyme form EP24.16m and EP24.16c in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
606A
-
a cleavage site mutant with altered substrate specificity
E502A
-
complete loss of enzymatic activity
G608A
-
site-directed mutagenesis, the mutation does not affect the overall fold of the protein, the mutant shows altered substrate specificity and kinetics with fluorogenic peptide substrates compared to the wild-type enzyme
H473A
-
complete loss of enzymatic activity
H474A
-
complete loss of enzymatic activity
H477A
-
complete loss of enzymatic activity
R470E/T499R
-
site-directed mutagenesis of the substrate recognition residues leads to a swap of substrate specificity from thimet oligopeptidase to neurolysin, EC 3.4.24.16, the mutant cleaves neurolysin sites, overview
Y606A
-
site-directed mutagenesis, the mutation does not affect the overall fold of the protein, the mutant shows altered substrate specificity and kinetics with fluorogenic peptide substrates compared to the wild-type enzyme
Y606F
-
site-directed mutagenesis, the mutation does not affect the overall fold of the protein, the mutant shows altered substrate specificity and kinetics with fluorogenic peptide substrates compared to the wild-type enzyme
Y606F
-
a cleavage site mutant with altered substrate specificity
Y610L
-
marked change in substrate specificity with 3fold higher binding efficiency to matrix metalloproteinase MMP-2/9 substrate (7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 with glutamic acid at the P2' amino acid position
additional information
-
dominant-negative G protein mutants used to selectively disrupt Gialpha- and Gbetagamma-mediated signaling pathways attenuated strain-dependent increases for the enzyme
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
plays an important role in endothelium-mediated vasoregulation
molecular biology
-
neurolysin can be used as a molecular tool for analysis of properties of cancer-producing matrix metalloproteinases MMP-2 and MMP-9, quantitative determination of cleavage activity of neurolysin toward MMPspecific fluorescence-quenching peptides, overview
additional information
-
agaricoglycerides are devoid of activity in a large number of biochemical and cellular assays, emphasizing their selectivity towards neurolysin
additional information
-
EP12.16 modulates intracellular, peptidergic signaling cascades through the type-2 BK receptor in physiologically relevant nociceptive system