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3.4.22.65: peptidase 1 (mite)

This is an abbreviated version!
For detailed information about peptidase 1 (mite), go to the full flat file.

Word Map on EC 3.4.22.65

Reaction

broad endopeptidase specificity =

Synonyms

allergen Der f 1, allergen Der f1, allergen Der p, allergen Der p 1, allergen Der p1, CO1.073, Der f 1, Der f1, Der p 1, Der p 7, Der p1, dust mite allergen Der p 7, dust mite peptidase allergen Der p 1, house dust mite allergen, house dust mite allergens, major house dust mite allergen, mite allergen, mite major group 1 allergens, pro-Der f1, ProDer f 1, ProDer p 1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.65 peptidase 1 (mite)

Renatured

Renatured on EC 3.4.22.65 - peptidase 1 (mite)

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
ProDer p 1, refolding after solubilization in urea
-
recombinant His-tagged fusion proteins encompassing Der p 2 with either mature or proDer p 1 from Escherichia coli inclusion bodies with purification via denaturating nickel affinity chromatography
recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea
recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea
recombinant proenzyme pro-Der f1 from Escherichia coli inclusion bodies, solubilization/degenration by 6 M urea, and refolding in refolding buffer containing 20 mM Tris.Cl pH 8.6, 1% glycerol, 1 mM EDTA, as well as 3 mM reduced glutathione, and 0.3 mM oxidized glutathione at a final concentration of 0.05 mg/ml, followed by gel filtration, method optimization, overview