Information on EC 3.4.22.65 - peptidase 1 (mite)

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The expected taxonomic range for this enzyme is: Dermatophagoides

EC NUMBER
COMMENTARY hide
3.4.22.65
-
RECOMMENDED NAME
GeneOntology No.
peptidase 1 (mite)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
broad endopeptidase specificity
show the reaction diagram
-
-
-
-
the proteolytic activity of Der f 1 is a major contributor to its allergenicity. Cysteine protease activity of Der p 1 enhances total IgE production
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2-aminobenzoyl)-Val-Ala-norLeu-Ser-Tyr(3-NO2)-Asp + H2O
?
show the reaction diagram
-
ADZ 50,059
-
-
?
2-aminobenzoic acid-Val-Ala-Nle-Ser-(3-nitro)-tyrosinyl-aspartamide + H2O
?
show the reaction diagram
-
-
-
-
?
alpha1-antitrypsin + H2O
?
show the reaction diagram
-
recombinant Der p 1 previously activated with L-cysteine or DTT
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
Azocoll + H2O
?
show the reaction diagram
butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumaryl-7-amide + H2O
butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin
show the reaction diagram
-
short synthetic substrate
-
-
?
butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
butyloxycarbonyl-Gln-Ala-Arg-MCA + H2O
butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
butyloxycarbonyl-Gln-Gly-Arg-MCA + H2O
butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
butyloxycarbonyl-Phe-Ser-Arg-MCA + H2O
butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
butyloxycarbonyl-Val-Leu-Lys-MCA + H2O
butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
CD23 + H2O
?
show the reaction diagram
CD25 + H2O
?
show the reaction diagram
chestnut cystatin + H2O
?
show the reaction diagram
-
specific proteolytic cleavage between Gly6 and Val7, giving rise to a noninhibitory processed protein. Not cleaved by Der f 1 from Dermatophygoides farinae
-
-
?
Der p 1 propiece + H2O
?
show the reaction diagram
-
potent competitive inhibitor of Der p 1. The Der p 1 propiece behaves as a substrate and is fully degraded during this interaction. The rapid inactivation of Der p 1 prodomain is a mechanism that may contribute to the potency of this allergen
-
-
?
human alpha1-antitrypsin + H2O
?
show the reaction diagram
-
cleaved by Der f 1-N53Q but not without its activation
-
-
?
human CD23 + H2O
?
show the reaction diagram
human CD25 + H2O
?
show the reaction diagram
lung surfactant protein A + H2O
?
show the reaction diagram
lung surfactant protein D + H2O
?
show the reaction diagram
N-Bz-Phe-Val-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Pro-Ala-7-amido-4-methylcoumarin
N-succinyl-Ala-Pro-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
more efficiently hydrolyzed than N-succinyl-Ala-Pro-Ala-7-amido-4-methylcoumarin
-
-
?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
N-tert-butoxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
N-tert-butoxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
pro-Der p 3 + H2O
Der p 3 + Der p 3 propeptide
show the reaction diagram
pro-Der p 6 + H2O
Der p 6 + Der p 6 propeptide
show the reaction diagram
pro-Der p 9 + H2O
Der p 9 + Der p 9 propeptide
show the reaction diagram
succinyl-Leu-Leu-Val-Tyr-MCA + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Z-Phe-Arg-4-methylcoumarin 7-amide + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CD23 + H2O
?
show the reaction diagram
-
Der p 1 in addition to being highly immunogenic may up-regulate IgE synthesis by virtue of its ability to cleave CD23
-
-
?
lung surfactant protein A + H2O
?
show the reaction diagram
-
degradation of lung surfactant protein A and lung surfactant protein D is associated with diminished binding to carbohydrates and to Dermatophagoides pteronyssinus allergen 1 itself and diminishes capacity to agglutinate bacteria. The degradation and consequent inactivation of lung surfactant protein A and lung surfactant protein D may be a novel mechanism to account for the potent allergenicity of the dust mite allergen
-
-
?
lung surfactant protein D + H2O
?
show the reaction diagram
-
degradation of lung surfactant protein A and lung surfactant protein D is associated with diminished binding to carbohydrates and to Dermatophagoides pteronyssinus allergen 1 itself and diminishes capacity to agglutinate bacteria. The degradation and consequent inactivation of lung surfactant protein A and lung surfactant protein D may be a novel mechanism to account for the potent allergenicity of the dust mite allergen
-
-
?
pro-Der p 3 + H2O
Der p 3 + Der p 3 propeptide
show the reaction diagram
-
activation
-
-
?
pro-Der p 6 + H2O
Der p 6 + Der p 6 propeptide
show the reaction diagram
-
activation
-
-
?
pro-Der p 9 + H2O
Der p 9 + Der p 9 propeptide
show the reaction diagram
-
activation
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
dithiothreitol dependent proteolytic activity
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
a flexible metal binding site is present in pro-Der p 1
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3S)-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxo-N-(4-sulfamoylbenzyl)pentanamide
-
-
(3S)-N-benzyl-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxopentanamide
-
-
(3S)-N-[4-[(dimethylamino)methyl]benzyl]-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxopentanamide
-
-
3-methyl-N-(naphthalen-1-ylcarbonyl)-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
3-methyl-N-(pyridin-4-ylcarbonyl)-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
3-methyl-N-(quinolin-4-ylcarbonyl)-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
3-methyl-N-(quinolin-4-ylcarbonyl)-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
3-methyl-N-(quinolin-4-ylcarbonyl)-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-([2-oxo-2-[4-(propan-2-yl)piperazin-1-yl]ethyl]amino)pentan-3-yl]-L-alaninamide
-
-
3-methyl-N-[(1-methylpiperidin-4-yl)carbonyl]-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
4-(2-aminoethyl) benzenesulfonyl fluoride
-
-
4-([[(3S)-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxopentanoyl]amino]methyl)-N-methylbenzamide
-
-
chestnut cystatin
-
inhibits the enzyme and greatly increases the larval mortality
-
cystatin A
Der p 1 allergen
-
ADZ 50,000, irreversible inactivation, active site titrant
-
Der p 1 prodomain
-
PP-02, recombinant, apparently competitive
-
Der p 1 propeptide
-
the propeptide of 80 amino acids plays an essential role in zymogen folding (as an intramolecular chaperone) and in the inhibition of its own enzymatic activity
-
Der p 1 propiece
-
potent competitive inhibitor of Der p 1. The Der p 1 propiece behaves as a substrate and is fully degraded during this interaction
-
egg-white cystatin
-
-
-
N-(naphthalen-1-ylcarbonyl)-L-phenylalanyl-N-[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-(cyclopropylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-([4-[(dimethylamino)methyl]benzyl]amino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-amino-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[(1H-pyrazol-4-ylmethyl)amino]pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[(4-sulfamoylbenzyl)amino]pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[(1R)-1-phenylethyl]amino]pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[(1S)-1-phenylethyl]amino]pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[4-(trifluoromethyl)benzyl]amino]pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1-(methylamino)-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-b,b-dimethyl-L-phenylalanyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1,2-dioxo-1-(pyrrolidin-1-yl)heptan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-1,2-dioxoheptan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-4,4-dimethyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-norvalinamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1-[[2-(3,4-dihydroisoquinolin-2(1H)-yl)-2-oxoethyl]amino]-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-1-[[2-(4,4-dimethylpiperazin-4-ium-1-yl)-2-oxoethyl]amino]-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-2-oxo-1-(pyrrolidin-1-yl)heptan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1,2-dioxo-1-(tetrahydro-2H-pyran-4-ylamino)pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1,2-dioxo-1-([2-oxo-2-[4-(propan-2-yl)piperazin-1-yl]ethyl]amino)pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[2-oxo-2-(piperidin-1-yl)ethyl]amino]pentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[(1-methylpiperidin-4-yl)amino]-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[[(2S)-1-(4-methylpiperazin-1-yl)-1-oxopropan-2-yl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[[2-(morpholin-4-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-benzoyl-L-valyl-N-[(3S)-1-(benzoyloxy)-2-oxoheptan-3-yl]-L-alaninamide
-
irreversible inhibitor
N-[(1,1-dimethylpiperidinium-4-yl)carbonyl]-3-methyl-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
N-[(1S)-2-[[(2S)-1-[[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]amino]-1-oxopropan-2-yl]amino]-2-oxo-1-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]benzamide
-
-
N-[(3,5-dimethyl-1H-pyrazol-4-yl)carbonyl]-3-methyl-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
-
-
phenylmethanesulfonyl fluoride
-
-
PTL11028
-
a highly potent and specific inhibitor, being effective against both purified protease and Der p 1 within HDM extract
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
the enzyme is completely dependent on preactivation with cysteine
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0154 - 14.4
(2-aminobenzoyl)-Val-Ala-norLeu-Ser-Tyr(3-NO2)-Asp
0.003
2-aminobenzoic acid-Val-Ala-Nle-Ser-(3-nitro)-tyrosinyl-aspartamide
-
pH 8.3
0.017 - 0.033
casein
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
2-aminobenzoic acid-Val-Ala-Nle-Ser-(3-nitro)-tyrosinyl-aspartamide
Dermatophagoides pteronyssinus
-
pH 8.3
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000031
chestnut cystatin
-
pH 6.5, 30C
-
0.000000005
cystatin A
-
pH 8.3
-
0.0000279
cystatin C
-
pH 8.3
-
0.00126
cystatin D
-
pH 8.3
-
0.0000148
cystatin E/M
-
pH 8.3
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000003
(3S)-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxo-N-(4-sulfamoylbenzyl)pentanamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000007
(3S)-N-benzyl-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxopentanamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000004
(3S)-N-[4-[(dimethylamino)methyl]benzyl]-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxopentanamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.00002
3-methyl-N-(naphthalen-1-ylcarbonyl)-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000013
3-methyl-N-(pyridin-4-ylcarbonyl)-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000018
3-methyl-N-(quinolin-4-ylcarbonyl)-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000013
3-methyl-N-(quinolin-4-ylcarbonyl)-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000003
3-methyl-N-(quinolin-4-ylcarbonyl)-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-([2-oxo-2-[4-(propan-2-yl)piperazin-1-yl]ethyl]amino)pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.00019
3-methyl-N-[(1-methylpiperidin-4-yl)carbonyl]-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000004
4-([[(3S)-3-([N-[(2S)-3,3-dimethyl-2-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanoyl]-L-alanyl]amino)-4-methyl-2-oxopentanoyl]amino]methyl)-N-methylbenzamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000051 - 0.0000569
Der p 1 prodomain
0.00002
N-(naphthalen-1-ylcarbonyl)-L-phenylalanyl-N-[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000009
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000014
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000017
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-(cyclopropylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000008
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-([4-[(dimethylamino)methyl]benzyl]amino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000006
N-benzoyl-3-methyl-L-valyl-N-[(3S)-1-amino-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000011
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[(1H-pyrazol-4-ylmethyl)amino]pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000006
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[(4-sulfamoylbenzyl)amino]pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000117
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[(1R)-1-phenylethyl]amino]pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000017
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[(1S)-1-phenylethyl]amino]pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000047
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[4-(trifluoromethyl)benzyl]amino]pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000024
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1-(methylamino)-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000014
N-benzoyl-3-methyl-L-valyl-N-[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000042
N-benzoyl-b,b-dimethyl-L-phenylalanyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000997
N-benzoyl-L-phenylalanyl-N-[(3S)-1,2-dioxo-1-(pyrrolidin-1-yl)heptan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000012
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000008 - 0.000017
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-1,2-dioxoheptan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.00917
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-4,4-dimethyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000018
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000164
N-benzoyl-L-phenylalanyl-N-[(3S)-1-(cyclohexylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-norvalinamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000006
N-benzoyl-L-phenylalanyl-N-[(3S)-1-[[2-(3,4-dihydroisoquinolin-2(1H)-yl)-2-oxoethyl]amino]-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000017
N-benzoyl-L-phenylalanyl-N-[(3S)-1-[[2-(4,4-dimethylpiperazin-4-ium-1-yl)-2-oxoethyl]amino]-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000065
N-benzoyl-L-phenylalanyl-N-[(3S)-2-oxo-1-(pyrrolidin-1-yl)heptan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000055
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1,2-dioxo-1-(tetrahydro-2H-pyran-4-ylamino)pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000004
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1,2-dioxo-1-([2-oxo-2-[4-(propan-2-yl)piperazin-1-yl]ethyl]amino)pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000014
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1,2-dioxo-1-[[2-oxo-2-(piperidin-1-yl)ethyl]amino]pentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000115
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[(1-methylpiperidin-4-yl)amino]-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.00001
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[[(2S)-1-(4-methylpiperazin-1-yl)-1-oxopropan-2-yl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000014
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000009
N-benzoyl-L-phenylalanyl-N-[(3S)-4-methyl-1-[[2-(morpholin-4-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000006
N-[(1,1-dimethylpiperidinium-4-yl)carbonyl]-3-methyl-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000005
N-[(1S)-2-[[(2S)-1-[[(3S)-4-methyl-1-[[2-(4-methylpiperazin-1-yl)-2-oxoethyl]amino]-1,2-dioxopentan-3-yl]amino]-1-oxopropan-2-yl]amino]-2-oxo-1-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]benzamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
0.000067
N-[(3,5-dimethyl-1H-pyrazol-4-yl)carbonyl]-3-methyl-L-valyl-N-[(3S)-1-(benzylamino)-4-methyl-1,2-dioxopentan-3-yl]-L-alaninamide
Dermatophagoides pteronyssinus
-
pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
wild-type pro-Der f1 is secreted to culture medium of baculovirus-transfeceted SF9 cells, but pro-N53Q is not
Manually annotated by BRENDA team
-
immunohistochemic localisation of the enzyme
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000 - 18000
-
recombinant form without N-glycosylation, gel filtration
17000
-
Der f 1-N53Q, gel filtration
18000
-
natural Der f 1, gel filtration
36000 - 60000
-
smear from 36 to 60 kDa, SDS-PAGE, recombinant Der p 1, expressed in Pichia pastoris
55000
-
average molecular weight, gel filtration, recombinant Der p 1, expressed in Pichia pastoris
additional information
-
recombinant Der p 1 and Der p 1 purified from Dermatophagoides pteronyssinus show differences in glycosylation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
Der p 7 has an elongated structure with two 4-stranded anti-parallel beta-sheets which wrap around a long C-terminal helix, NMR analysis of His-tagged Der p 7, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
4C, 45% monomethyl polyethylene glycole 5000 or 2000 in 50 mM sodium acetate (pH 5.5), 1.9 A resolution, typical papain-family fold, exhibits a magnesium binding site
-
recombinant Der p 7-MBP fusion protein, mixing of 0.001 ml of 27 mg/mL protein in 25 mM HEPES, pH 7.4, 75 mM NaCl, and 5 mM maltose, with 00.001 ml of reservoir solution containing 42.5 mM Tris pH 8.5, 12.75% PEG 4000, 85 mM lithium sulfate, and 7.5% glycerol, 4C, 1 week, X-ray diffraction structure determination and analysis at 2.35 A resolution, molecular replacement
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
most of the reDer f1 E(-1)K produced by the transformants is degraded when culture is shaken vigorously. The degradation of reDer f1 E(-1)K is suppressed when it is shaken gently
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
ammonium sulfate precipitation, gel filtration, ion exchange chromatography
-
anion exchange and gel filtration
-
column chromatography
-
culture supernatants recovered, buffer exchange by gel filtration, equilibration with 100 mM sodium acetate buffer (pH 4)
-
dialysis, cationic exchange on SP-Sepharose, recombinant Der p 1 dialysis, benzamidine column, dialysis
-
immunoaffinity chromatography using immobilized 4C1 anti-Der p 1 monoclonal antibody
-
mature form purified by anion exchange and gel filtration chromatography
-
mature forms were purified by anion-exchange and gel filtration chromatography, proforms were purified by means of the same methods as the mature forms but without activation by dialysis against acidic buffer, natural Der f 1 was purified from whole culture extracts of Dermatophagoides farinae
-
proform secreted in Pichia pastoris culture supernatant and converted into their prosequence-removed mature form, anion exchange column and gel filtration
-
recombinant Der p 7-MBP fusion protein Der p 7 from Escherichia coli Rosetta2(DE3) pLacI cells by amylose affinity chromatography and gel filtration. Recombinant His6-tagged Der p 7 from Escherichia coli Rosetta2(DE3) pLacI cells by alternating nickel affinity and anion exchange chromatography
-
recombinant His-tagged fusion proteins by nickel affinity chromatography using a His-Trap column under denaturing conditions with 20 m M sodium phosphate, pH 7.0, 0.5 M NaCl, 0.05% Tween 20, 8 M urea, 1 mM DTT, 0.03 M imidazole as binding buffer, and 20 mM sodium phosphate, pH 7.8, 0.05% Tween 20, 8 M urea, 1 mM DTT, 1 M imidazole as elution buffer
-
recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves by affinity chromatography to homogeneity under denaturing conditions
-
recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves by affinity chromatography to homogeneity under denaturing conditions
recombinant proenzyme pro-Der f1 from Escherichia coli after solubilization from inclusion bodies and refolding by dialysis and affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
construction of an isopropyl-beta-D-thiogalactopyranoside-inducible expression plasmid to produce the pro-form of Der f1 in Escherichia coli
-
Der p 1.0124 (according to the IUIS-WHO Allergen Nomenclature Subcommittee)
-
DNA and amino acid sequence determination and analysis of the cDNA sequence encoding proenzyme pro-Der f1, recombinant expression od Strep-tagged proenzyme in Escherichia coli in inclusion bodies from vector pET-44a
-
DNA fragment encoding precursor-type recombinant Der f1 E(-1)K, which has the C-terminal amino acid of the pro-sequence, Glu, changed to Lys, expression in Aspergillus oryzae
-
expressed in Pichia pastoris
-
expressed in Saccharomyces cerevisiae
-
expression and maturation of prepro-Der p 1 and its mutants in Pichia pastoris. Maturation systems using Pro-Der p 1 without the prodomain glycosylation are useful for the efficient preparation of a recombinant mature allergen; expression in Pichia pastoris
-
expression in Escherichia coli
-
expression in Pichia pastoris X-33
-
expression of Der p 7-MBP fusion protein and His6-tagged Der p 7 in Escherichia coli Rosetta2(DE3) pLacI cells
-
expression of His-tagged fusion proteins encompassing Der p 2 with either mature or proDer p 1 in Escherichia coli strain BL21 (DE3) and Origami2 (DE3), or in Pichia pastoris strain GS115 or X33. Four fusion proteins are expressed in Escherichia coli as inclusion bodies, whereas only chimeras comprising proDer p 1 are obtained in yeast
-
I-Der p 1 gene, cDNA isolation, DNA and amino acid sequence determination and analysis, detection of 11 polymorphisms to the sequence of peptidase 1, DERP1, UniProt ID P08176, sequence comparison, PVX-mediated expression of immunologically active mature form I-rDer p 1 in Nicotiana benthamiana plants using a Agrobacterium tumefaciens strain GV3101-mediated transfection strategy, the recombinant enzyme comprises the N-terminal 222 amino acids which corresponds to the mature form of I-Der p 1 after proteolytic cleavage, the C-terminus is expressed as FLAG-tagged protein
preparation described elsewhere, proform secreted in Pichia pastoris culture supernatant and converted into their prosequence-removed mature form, recombinant protein exhibits cysteine protease activity but no serine protease activity
-
production of recombinant enzyme in insect cells using a baculovirus expression system
-
recombinant Der f1 that is produced using a baculovirus expression sytem contains pro-sequences of different length. Most of theses can be removed by acid treatment. The N-terminal amino acids of the acid-treated recombinant enzyme are uniform. A method for processing of the pro-sequence has been developed by producing reDer f1 E(-1)K with baculovirus expression system in which the carboxy terminal amino acid of the pro-sequence (glutamate) is replaced by lysine. Addition of lysylendopeptidase into the culture medium leads to processing of the pro-sequence of reDer f1 E(-1)K
-
recombinant expression in Pichia pastoris
-
recombinant expression of C-terminally His6-tagged chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, in leaves of Nicotiana benthamiana via transfection with Agrobacterium tumefaciens strain GV3101
-
recombinant expression of C-terminally His6-tagged wild-type enzyme and chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, in leaves of Nicotiana benthamiana via transfection with Agrobacterium tumefaciens strain GV3101
recombinant ProDer p 1 from CHO cells
-
recombinant proforms secreted into culture supernatant of Pichia pastoris cells and converted into prosequence-removed mature form Der p 1-WT
-
recombinant proforms were secreted into the culture of Pichia pastoris transfectant cells and converted into the prosequence-removed mature forms by means of dialysis against an acidic buffer
-
the preproforms of Der f 1 and a mutant N53Q, whose consensus motif for N-glycosylation is disrupted, are expressed in Pichia pastoris
-
the propiece of Der p 1 is PCR cloned and expressed in Escherichia coli
-
wild-type and mutant enzyme N52Q, expression in Pichia pastoris
-
wild-type pro-Der f1 is secreted to culture medium of baculovirus-transfeceted SF9 cells, pro-N53Q is not secreted in insect Sf9 cells although secreted in Pichia pastoris
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C104S/N53Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C118S/N53Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C32S/C72S/N63Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C32S/N53Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C35S
-
catalytic site mutant, expressed in Saccharomyces cerevisiae
C35S/N53Q
-
proform of mutant enzyme is efficiently secreted into culture medium. The secreted pro-C35S/N53Q is converted to the mature form by treatment in acidic conditions. Cysteine protease activity of the processed C35S/N53Q is lower than that of native Der f1 but significantly higher than that of the proenzyme
C4S/N53Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C66S/C104S/N53Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C66S/N53Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C72S/N53Q
-
proform of mutant enzyme is not efficiently secreted into culture medium
C132V
-
site-directed mutagenesis, Der p 1 catalytic site mutant
additional information
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ProDer p 1, refolding after solubilization in urea
-
recombinant His-tagged fusion proteins encompassing Der p 2 with either mature or proDer p 1 from Escherichia coli inclusion bodies with purification via denaturating nickel affinity chromatography
-
recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea
-
recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea
recombinant proenzyme pro-Der f1 from Escherichia coli inclusion bodies, solubilization/degenration by 6 M urea, and refolding in refolding buffer containing 20 mM Tris.Cl pH 8.6, 1% glycerol, 1 mM EDTA, as well as 3 mM reduced glutathione, and 0.3 mM oxidized glutathione at a final concentration of 0.05 mg/ml, followed by gel filtration, method optimization, overview
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
drug development
-
the enzyme is a target for design and development of specific inhibitors for treatment of allergic asthma
medicine
pharmacology
-
production of recombinant enzymatically and immunologically enzyme for deciphering of immunotherapy mechanisms